Carboplatin-loaded zeolitic imidazolate framework-8: Induction of antiproliferative activity and apoptosis in breast cancer cell
breast cancer; cell death; cytotoxicity; drug delivery; pH sensitive
The challenge with breast cancer is its ongoing high prevalence and difficulties in early detection and access to effective care. A solution lies in creating tailored metalorganic frameworks to encapsulate anticancer drugs, enabling precise and targeted treatment with less adverse effects and improved effectiveness. Zeolitic imidazolate framework-8 (ZIF-8) and carboplatin (CP)-loaded ZIF-8 were synthesized and characterized using various analytical techniques. High Resolution-transmission electron microscopy of ZIF-8 and CP@ZIF-8 indicates that the particles had a spherical shape and were nanosized. The drug release rate of CP is 98% under an acidic medium (pH 5.5) because of the dissolution of ZIF-8 into its coordinating ions, whereas 35% in a physiological medium (pH 7.4) with the addition of CP, the high porosity, and pore diameter of ZIF-8 decrease from 1243 to 1041m2/g. Breast cancer MCF-7 cells were shown greater IC50 in CP@ZIF-8 (15.013.03g/mL) than free CP (34.984.25g/mL) in an in vitro cytotoxicity assessment. The cytotoxicity of the CP@ZIF-8 against MCF-7 cells was studied using the methylthiazolyldiphenyl-tetrazolium bromide method. The morphological changes were examined using fluorescent staining (acridine orangeethidium bromide and Hoechst 33258) methods. The comet assay assessed the DNA fragmentation (single-cell gel electrophoresis). The results from the study revealed that CP@ZIF-8 can be used in the treatment of breast cancer. 2024 International Union of Biochemistry and Molecular Biology, Inc.
Ganapathy, Saravanan; Bharathi, Muruganantham; Hirad, Abdurahman Hajinur; Alarfaj, Abdullah A.; Thangavelu, Indumathi; Arulselvan, Palanisamy; Jaganathan, Ravindran; Ravindran, Rajeswari; Suriyaprakash, Jagadeesh; Boopathi, Thalakulam Shanmugam
Biotechnology and Applied Biochemistry;Volume;72;Issue;3;pp.683-694
https://doi.org/10.1002/bab.2689 https://www.scopus.com/pages/publications/85208035599?origin=resultslist
Ganapathy S., Department of Biochemistry, K.S. Rangasamy College of Arts and Science, Tamil Nadu, Tiruchengode, India; Bharathi M., Centre for Bioinformatics, Department of Biochemistry, Karpagam Academy of Higher Education, Tamil Nadu, Coimbatore, India; Hirad A.H., Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia; Alarfaj A.A., Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia; Thangavelu I., Department of Chemistry, CHRIST (Deemed to be University), Bangalore, India; Arulselvan P., Department of Chemistry, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences (SIMATS), Saveetha University, Tamil Nadu, Chennai, India; Jaganathan R., Preclinical Department, Faculty of Medicine, Universiti Kuala Lumpur, Royal College of Medicine Perak (UniKL-RCMP), Perak, Ipoh, Malaysia; Ravindran R., Preclinical Department, Faculty of Medicine, Universiti Kuala Lumpur, Royal College of Medicine Perak (UniKL-RCMP), Perak, Ipoh, Malaysia; Suriyaprakash J., Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, School of Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou, China; Boopathi T.S., Department of Chemistry, Amrita School of Physical Sciences, Amrita Vishwa Vidyapeetham, Coimbatore, India, Functional Materials Laboratory, Amrita School of Engineering, Amrita Vishwa Vidyapeetham, Coimbatore, India