Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Faculty Publications Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Creator An entity primarily responsible for making the resource Thangavel, Myithili; Sneha, Michael Joe Xavier; Mani, Israel; Surendrababu, Akash; Rajapriya, Pandy; Arulselvan, Palanisamy; Alarfaj, Abdullah A.; Thangavelu, Indumathi; Pandi, Mohan Title A name given to the resource Diversity of Endophytic Fungi in Plant Species: Traditional vs. High-Throughput Sequencing Approaches Date A point or period of time associated with an event in the lifecycle of the resource 01-01-2025 Source A related resource from which the described resource is derived Chemistry and Biodiversity;Volume;22;Issue;6;Article No.;e202402792; Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.1002/cbdv.202402792" target="_blank" rel="noreferrer noopener">https://doi.org/10.1002/cbdv.202402792</a> <br /><br /><a href="https://www.scopus.com/pages/publications/105006575408?origin=resultslist" target="_blank" rel="noreferrer noopener">https://www.scopus.com/pages/publications/105006575408?origin=resultslist</a> Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Thangavel M., Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Tamil Nadu, Madurai, India; Sneha M.J.X., Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Tamil Nadu, Madurai, India; Mani I., Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Tamil Nadu, Madurai, India; Surendrababu A., Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Tamil Nadu, Madurai, India; Rajapriya P., Department of Zoology, M.S.S. Wakf Board College, Tamil Nadu, Madurai, India; Arulselvan P., Department of Biochemistry, Karpagam Academy of Higher Education (Deemed to be University), Tamil Nadu, Coimbatore, India; Alarfaj A.A., Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia; Thangavelu I., Department of Chemistry, CHRIST (Deemed to be University), Bangalore, India; Pandi M., Department of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Tamil Nadu, Madurai, India Description An account of the resource The plant microbiome significantly impacts plant life, with fungi playing a crucial role in shaping interactions and classifications. Advances in cultivation technologies have refined fungal classification, and research highlights the vital connection between endophytic fungi and their plant hosts. The present study employs morphological and phylogenetic techniques, predicting the Internal Transcribed Spacer 2 (ITS2) secondary structure and using next-generation sequencing (NGS) data to detect fungal endophytes in plant leaves via both traditional and conventional approaches. The research area, with its hot semi-arid environment and red and black soils, supports drought-resistant plants like Senna auriculata, Ziziphus mauritiana, and Catunaregam spinosa, known for their medicinal properties. These plants, rich in antioxidants, play a vital role in traditional medicine and highlight the region's rich ethno-botanical heritage. The culture-dependent study on the foliage yielded a total of 17 isolates from S. auriculata and 16 each from both C. spinosa and Z. mauritiana. The most common genera, Alternaria and Nigrospora, account for 18.3% of all isolated endophytic fungi. Three plants were colonized with Nigrospora and Lasiodiplodia, and their morphotypes were determined using ITS2 secondary structure prediction. Recent ecological studies highlight unculturable taxa, or dark taxa, where many species cannot sporulate or be cultured, emphasizing the need for High - Throughput Sequencing (HTS) approaches. The study gathered 68,791 reads from S. auriculata with 101 operational taxonomic units (OTUs), 58,620 from C. spinosa with 219 OTUs, and 66,087 from Z. mauritiana with 193 OTUs, with the majority of OTUs related to Colletotrichum (69%) and a minimum of Myrmaecium (2%). A total of 49 fungal isolates were obtained from traditional methods, whereas 513 fungal OTUs were retrieved through HTS methods, confirming the presence of a highly abundant fungus population in plant samples. The study reveals that using the ITS short amplicon sequencing technique provides distinct insights into endophytic fungal communities in three plant samples. In conclusion, analyzing plant fungal components using a combination of culture-dependent and culture-independent techniques may be a novel strategy. 2025 Wiley-VHCA AG, Zurich, Switzerland. Subject The topic of the resource amplicon; Internal Transcribed Spacer (ITS); metagenomic; Operational Taxonomic Unit (OTU); phylogenetic Publisher An entity responsible for making the resource available John Wiley and Sons Inc Relation A related resource ISSN: 16121872; CODEN: CBHIA Language A language of the resource English Type The nature or genre of the resource Article Rights Information about rights held in and over the resource Restricted Access; Hardcopy may be available in the library Format The file format, physical medium, or dimensions of the resource online