Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Faculty Publications Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Creator An entity primarily responsible for making the resource Spandana, K.J.; Rodrigues, Wilson Joel; Ghate, Sudeep D.; Rao, R. Shyama Prasad; Chandrashekar, K.R.; Bhagya, N. Title A name given to the resource In Vitro Cytotoxic Potential and Integrated Network Pharmacology, Molecular Docking and Molecular Dynamic Approaches to Decipher the Mechanism of Gymnostachyum febrifugum Benth., in the Treatment of Breast Cancer Date A point or period of time associated with an event in the lifecycle of the resource 01-01-2025 Source A related resource from which the described resource is derived Applied Biochemistry and Biotechnology;Volume;197;Issue;5;pp.2887-2909 Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.1007/s12010-024-05173-1" target="_blank" rel="noreferrer noopener">https://doi.org/10.1007/s12010-024-05173-1</a> <br /><br /><a href="https://www.scopus.com/pages/publications/85217202406?origin=resultslist" target="_blank" rel="noreferrer noopener">https://www.scopus.com/pages/publications/85217202406?origin=resultslist</a> Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Spandana K.J., Yenepoya Research Centre, Yenepoya (Deemed to be University), Karnataka, Mangalore, 575018, India; Rodrigues W.J., JSS Academy of Higher Education and Research, (Deemed to be University), Shivarathreeshwara Nagara, Karnataka, Mysuru, 570015, India; Ghate S.D., Central Research Laboratory, NITTE (Deemed to be University), K.S. Hegde Medical Academy, Mangalore, 575018, India, Center for Bioinformatics, NITTE (Deemed to be University), Mangalore, 575018, India; Rao R.S.P., Central Research Laboratory, NITTE (Deemed to be University), K.S. Hegde Medical Academy, Mangalore, 575018, India, Center for Bioinformatics, NITTE (Deemed to be University), Mangalore, 575018, India; Chandrashekar K.R., Yenepoya Pharmacy and Ayush Research Centre (YEN PARC), Yenepoya (Deemed to be University), Karnataka, Deralakatte, Mangalore, 575018, India; Bhagya N., Yenepoya Research Centre, Yenepoya (Deemed to be University), Karnataka, Mangalore, 575018, India Description An account of the resource Gymnostachyum febrifugum, a less-known ethnomedicinal plant from the Western Ghats of India, is used to treat various diseases and serves as an antioxidant and antibacterial herb. The present study aims to profile the cytotoxic phytochemicals in G. febrifugum roots using GCMS/MS, in vitro confirmation of cytotoxic potential against breast cancer and an in silico study to understand the mechanism of action. Phytochemical profiling using GCMS/MS showed the presence of eight cytotoxic molecules with lupeol in high abundance. A potent cytotoxic effect of G. febrifugum roots against breast cancer was also observed with antiproliferation, antimigration, inhibition in colony formation and death of breast cancer cells. Further, the cytotoxic potential of the plant was confirmed with the apoptosis of cells as observed in the flow cytometry. In silico network pharmacology, GO and KEGG analysis suggested the modulation of proteins of MAPK, PI3K-AKT and apoptosis pathways by lupeol to induce cytotoxicity in breast cancer. Further, dynamic simulation revealed MAPK and AKT as the major targets for lupeol. Our studies comprehensively elucidated the role of lupeol, a major phytochemical in G. febrifugum to induce cytotoxicity against breast cancer by targeting major cancer signaling pathways, providing a promising strategy and scientific basis to explore lupeol in targeted cancer therapy. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2025. Subject The topic of the resource Apoptosis; Cytotoxicity; GCMS/MS; Gymnostachyum febrifugum; MD simulation; Molecular docking; Network pharmacology Publisher An entity responsible for making the resource available Springer Relation A related resource ISSN: 2732289; Language A language of the resource English Type The nature or genre of the resource Article Rights Information about rights held in and over the resource Restricted Access; Hardcopy may be available in the library Format The file format, physical medium, or dimensions of the resource online