Exploring boronate-appended hyperbranched amino-functionalized dendrimer-empowered sensors for the potential recognition of FSH in age-categorized human plasma samples
Biosensor; Clinical sample; Dendrimer; Electrochemistry; Gold chip
Boronic acids can act as ideal saccharide receptors as they possess a high affinity for diols and readily form cyclic-boronate esters when reacting in an aqueous medium. Here, we present hydrophilic amino-functionalized boronic acid dendrimer (Af-BAD) for the first time, with significantly enhanced sensitivity towards Follicle Stimulating Hormone (FSH) detection. In this study, newly synthesized Af-BAD was dip-coated on a gold substrate to create an impedance-type sensing working electrode. The effects of Af-BAD coating on the gold chip, the sensing properties for FSH recognition, sensitivity, and stability were measured by the charge transfer resistance across the electrochemical setup. The impedimetric measurements were conducted in the presence of [Fe(CN)6]3-/[Fe(CN)6]4- redox reporter at pH 7.4. The increments in the charge-transfer resistance were monitored upon increasing the FSH concentrations from 25 fg/mL to 100 pg/mL. The device achieved good sensitivity with a calculated detection limit of 4.01 fg/mL and acceptable linearity. The observed behavior was linear concerning the tested concentrations. An attempt at a real application to serum samples was also successfully conducted. Meanwhile, the level of tolerance of boronic acid dendrimer with other competing glycoproteins and monosaccharides was also tested. In this study, we also compared human plasma FSH levels in female oral cancer patients and normal controls using the Af-BAD modified device and the clinically used ELISA method. With a sound understanding of boronate materials and their affinity, amino functionalized multi-boronic acid dendrimer was developed as a highly selective conjugate toward glycoprotein FSH detection. Copyright 2025. Published by Elsevier Ltd.
Palanisamy, Sathyadevi; Vijayaraghavan, Priya; Wang, Yen-Yun; Yao, Yi-Wen; Huang, Bo-Cheng; Chen, Wen-Liang; Kao, Chai-Lin; Yuan, Shyng-Shiou F.; Wang, Yun-Ming
Applied Materials Today;Volume;48;Issue;;Article No.;103006;
Restricted Access; Hardcopy may be available in the library
https://doi.org/10.1016/j.apmt.2025.103006 https://www.scopus.com/pages/publications/105023144334?origin=resultslist
Palanisamy S., Department of Chemistry, National Taiwan Normal University, Taipei, 11677, Taiwan, Department of Biological Science and Technology, Institute of Molecular Medicine and Bioengineering, B), National Yang Ming Chiao Tung University, Hsinchu, 300, Taiwan; Vijayaraghavan P., Department of Biological Science and Technology, Institute of Molecular Medicine and Bioengineering, B), National Yang Ming Chiao Tung University, Hsinchu, 300, Taiwan, Department of Chemistry, CHRIST University, Karnataka, Bengaluru, 560029, India; Wang Y.-Y., College of Dental Medicine, School of Dentistry, Kaohsiung Medical University, Kaohsiung, 807, Taiwan, Department of Medical Research, Kaohsiung Medical University Hospital, Kaohsiung, 807, Taiwan; Yao Y.-W., Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung, 807, Taiwan; Huang B.-C., Department of Surgery, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, 807, Taiwan, Drug Development and Value Creation Research Center, Kaohsiung Medical University, Kaohsiung, 807, Taiwan; Chen W.-L., Department of Biological Science and Technology, Institute of Molecular Medicine and Bioengineering, B), National Yang Ming Chiao Tung University, Hsinchu, 300, Taiwan; Kao C.-L., Department of Medicinal and Applied Chemistry, Kaohsiung Medical University, Kaohsiung, 807, Taiwan; Yuan S.-S.F., Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung, 807, Taiwan, Faculty and College of Medicine, Translational Research Center and Department of Obstetrics and Gynecology, Kaohsiung Medical University, Kaohsiung Medical University Hospital, Kaohsiung, 807, Taiwan; Wang Y.-M., Department of Biological Science and Technology, Institute of Molecular Medicine and Bioengineering, B), National Yang Ming Chiao Tung University, Hsinchu, 300, Taiwan, Center for Neurotechnology and Assistive Technology, Kaohsiung Medical University, Kaohsiung, 807, Taiwan