Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Faculty Publications Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Creator An entity primarily responsible for making the resource Gupta, Himanshu; Singh, Ashutosh; Gupta, Ashish Title A name given to the resource Cancer-associated mutation at glycine 400 in TIP60 disrupt its phase separation property and catalytic activity resulting in compromised DNA damage repair function of the cell Date A point or period of time associated with an event in the lifecycle of the resource 01-01-2025 Source A related resource from which the described resource is derived Biochemical and Biophysical Research Communications;Volume;753;Issue;;Article No.;151457; Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.1016/j.bbrc.2025.151457" target="_blank" rel="noreferrer noopener">https://doi.org/10.1016/j.bbrc.2025.151457</a> <br /><br /><a href="https://www.scopus.com/pages/publications/85217924290?origin=resultslist" target="_blank" rel="noreferrer noopener">https://www.scopus.com/pages/publications/85217924290?origin=resultslist</a> Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Gupta H., Epigenetics and Human Disease Laboratory, Centre of Excellence in Epigenetics, Department of Life Sciences, Shiv Nadar Institution of Eminence, Deemed to be University, Delhi-NCR, Uttar Pradesh, 201314, India; Singh A., Department of Life Sciences, Shiv Nadar Institution of Eminence, Deemed to be University, Delhi-NCR, Uttar Pradesh, 201314, India; Gupta A., Epigenetics and Human Disease Laboratory, Centre of Excellence in Epigenetics, Department of Life Sciences, Shiv Nadar Institution of Eminence, Deemed to be University, Delhi-NCR, Uttar Pradesh, 201314, India Description An account of the resource TIP60 is a tumor suppressor with histone acetyltransferase (HAT) activity, playing a crucial role in regulating chromatin architecture by acetylating histones to enhance accessibility for other regulatory factors. Its function is vital for several key cellular processes, including DNA damage repair, apoptosis, and autophagy. While the downregulation of TIP60 has been associated with various cancers, the effects of naturally occurring mutations in TIP60 on its function in malignancies remain poorly understood. In this study, we explored how cancer-related mutations in TIP60 impact its structure and function. Several deleterious and destabilizing missense mutations were identified and analyzed for structural changes. Molecular dynamics simulations revealed alterations in protein conformational stability and radius of gyration due to these mutations, supported by significant changes in TIP60's solvent accessibility and intramolecular hydrogen bonding. Biochemical assays with recombinant proteins showed a loss of catalytic activity in the G400W mutant. Live cell imaging indicated abnormal localization of the G400W mutant within the nucleus. Additionally, we observed aberrant phase separation of TIP60 caused by the G400W mutation. Notably, the G400W mutation impairs TIP60's catalytic function, preventing effective DNA repair and leaving the genome vulnerable to further mutations. Our findings highlight cancer-associated mutations in TIP60 that may contribute to the molecular mechanisms underlying cancer initiation and progression. 2025 Elsevier Inc. Subject The topic of the resource DNA damage repair; Missense mutation; TIP60; Tumor suppressor Publisher An entity responsible for making the resource available Elsevier B.V. Relation A related resource ISSN: 0006291X; CODEN: BBRCA Language A language of the resource English Type The nature or genre of the resource Article Rights Information about rights held in and over the resource Restricted Access; Hardcopy may be available in the library Format The file format, physical medium, or dimensions of the resource online