ItemArticle

Bioprospecting of fish scale waste as a cost effective substrate for crude protease production using Stenotrophomonas koreensis SH32 and its multifunctional applications: A sustainable strategy for circular bioeconomy

Title
Bioprospecting of fish scale waste as a cost effective substrate for crude protease production using Stenotrophomonas koreensis SH32 and its multifunctional applications: A sustainable strategy for circular bioeconomy
Creator
Suresh, Sreehari; Umesh, Mridul; Nag, Anish; James, Nilina; Santhosh, Adhithya Sankar; Stanly, Liya Merin
Description
In this study Stenotrophomonas koreensis SH32 bacterial strain previously isolated from waste dumped soil was screened for protease production through qualitative and quantitative methods. The protease enzyme production in based media was optimised using response surface methodology (RSM). Under optimised conditions 295.11 IU/mL of protease activity was recorded from the crude enzyme that marked a 5.76 fold increase in protease activity as compared to unoptimised condition (51.21 IU/mL). The crude enzyme was further explored for its dehairing activity using goat skin, destaining activity using chicken blood stained cloth and metal recovery property from old X-ray films. Further the plant growth promoting characteristics and antagonistic activity of the proteolytic strain against common phytopathogenic fungi was evaluated. The results suggested that the bacteria was positive for indole acetic acid, siderophore and ammonia production. The bacterial culture also showed antagonism against Cladosporium tennuisimum, Talaromyces albobiverticillius and Fusarium solani. The plant growth promotion activity of the bacteria was further confirmed by plant growth test using Solanum lycopersicum amended with fish scale hydrolysate produced through bacterial fermentation. A significant increase in length (8.3 cm) was observed in plants treated with fish scale hydrolysate as compared to the control (6.5 cm). 2025
Source
Biomass and Bioenergy;Volume;197;Issue;;Article No.;107811;
Date
01-01-2025
Publisher
Elsevier Ltd
Subject
Dehairing; Destaining; Fish scale hydrolysate; Optimization; Plant growth promotion; Protease
Coverage
Suresh S., Centre for Global Health Research, Saveetha Medical College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Tamil Nadu, Thandalam, 602105, India; Umesh M., Department of Life Sciences, Christ University, Hosur Road, Karnataka, Bangalore, 560029, India; Nag A., Department of Life Sciences, Christ University, Hosur Road, Karnataka, Bangalore, 560029, India; James N., Department of Life Sciences, Christ University, Hosur Road, Karnataka, Bangalore, 560029, India; Santhosh A.S., Department of Life Sciences, Christ University, Hosur Road, Karnataka, Bangalore, 560029, India; Stanly L.M., Department of Life Sciences, Christ University, Hosur Road, Karnataka, Bangalore, 560029, India
Rights
Restricted Access; Hardcopy may be available in the library
Relation
ISSN: 9619534; CODEN: BMSBE
Format
online
Language
English
Type
Article

Citation

Suresh, Sreehari; Umesh, Mridul; Nag, Anish; James, Nilina; Santhosh, Adhithya Sankar; Stanly, Liya Merin, “Bioprospecting of fish scale waste as a cost effective substrate for crude protease production using Stenotrophomonas koreensis SH32 and its multifunctional applications: A sustainable strategy for circular bioeconomy,” CHRIST (Deemed To Be University) Institutional Repository, accessed June 19, 2026, https://archives.christuniversity.in/items/show/22201.

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