Green synthesis, characterization, and biological applications of silver nanoparticles from Pachira glabra leaf extract
- Title
- Green synthesis, characterization, and biological applications of silver nanoparticles from Pachira glabra leaf extract
- Creator
- Elias, Elsha M.; Meganathan, Gomathy; Pappuswamy, Manikantan
- Description
- Green synthesis of silver nanoparticles (AgNPs) offers an environmentally sustainable approach to nanoparticles (NPs) production, utilizing plant extracts as reductant and stabilizer. This method helps minimizes the involvement of toxic chemicals, making it an economical and eco-friendly substitute to traditional synthetic techniques. The objective of this study is to synthesize and analyse AgNPs formed from Pachira glabra Pasq. leaf extract and to evaluate their biological applications. This study presents the first report on the green synthesis of AgNPs using P. glabra leaf extract, demonstrating its antibacterial, antioxidant, and cytotoxic potential. In this study, 1 mM silver nitrate (AgNO3) was used for synthesizing AgNPs. The shift in colour of the solution from pale green to brown indicates NP synthesis which was further confirmed by spectrophotometric analysis, exhibiting a peak at 424 nm. Fourier-transform infrared spectroscopy (FTIR) analysis was done to recognize the functional groups present in both P. glabra leaf extract and AgNPs synthesized. FTIR analysis revealed key functional groups such as hydroxyl, carbonyl, and amine groups. Peaks were observed in the range of 504 ?3351 cm?1. Dynamic Light Scattering (DLS) and zeta potential of biosynthesized AgNPs showed the size distribution and stability in suspension. Field Emission Scanning Electron Microscopy (FE-SEM) revealed that the biosynthesized AgNPs exhibited both cubic and spherical morphologies. Energy-dispersive X-ray spectroscopy (EDS) confirmed the presence and distribution of silver (Ag) and other elemental contents. The crystallinity of biosynthesized AgNPs was confirmed through X-ray diffraction (XRD) pattern. These results helped to characterize the biosynthesized AgNPs. Antibacterial activity of AgNPs was tested against Gram-negative Escherichia coli (MTCC 443) and Gram-positive Staphylococcus aureus (MTCC 3160) bacterial strains, with the AgNPs showing maximum effectiveness against E. coli, exhibiting an inhibition zone of 6.5 1.5 mm. The DPPH assay was used to evaluate antioxidant activity, and the biosynthesized AgNPs demonstrated a scavenging activity of 82.99 %, showing strong antioxidant potential compared to the standard. The cytotoxicity of both AgNPs and P. glabra leaf extract was tested against HCT-116 colorectal carcinoma cell line (ATCC-CCL-247). 2025 The Authors
- Source
- Microbe (Netherlands);Volume;7;Issue;;Article No.;100413;
- Date
- 01-01-2025
- Publisher
- Elsevier B.V.
- Subject
- Antimicrobial; Antioxidant; Characterization; Cytotoxicity; Green synthesis; Pachira glabra; Silver nanoparticle
- Coverage
- Elias E.M., Department of Life science, CHRIST (Deemed to be University), Karnataka, Bangalore, India; Meganathan G., Department of Life science, CHRIST (Deemed to be University), Karnataka, Bangalore, India; Pappuswamy M., Department of Life science, CHRIST (Deemed to be University), Karnataka, Bangalore, India
- Rights
- All Open Access; Gold Open Access
- Relation
- ISSN: 29501946;
- Format
- online
- Language
- English
- Type
- Article
Collection
Citation
Elias, Elsha M.; Meganathan, Gomathy; Pappuswamy, Manikantan, “Green synthesis, characterization, and biological applications of silver nanoparticles from Pachira glabra leaf extract,” CHRIST (Deemed To Be University) Institutional Repository, accessed June 18, 2026, https://archives.christuniversity.in/items/show/22392.