Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Faculty Publications Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Creator An entity primarily responsible for making the resource Kim, Ye-Won; Lee, Han-Sol; Murthy, Hosakatte Niranjana; Lee, Hyoshin; Park, So-Young Title A name given to the resource In vitro storage under slow growth, plant regeneration, and ex vitro acclimatization of Ligusticum officinale (Makino) Kitag Date A point or period of time associated with an event in the lifecycle of the resource 01-01-2026 Source A related resource from which the described resource is derived South African Journal of Botany;Volume;195;pp.152-159 Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.1016/j.sajb.2026.04.052" target="_blank" rel="noreferrer noopener">https://doi.org/10.1016/j.sajb.2026.04.052</a> <br /><br /><a href="https://www.scopus.com/pages/publications/105039822547?origin=resultslist" target="_blank" rel="noreferrer noopener">https://www.scopus.com/pages/publications/105039822547?origin=resultslist</a> Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Kim Y.-W., Department of Horticultural Science, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, South Korea; Lee H.-S., Department of Horticultural Science, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, South Korea; Murthy H.N., Department of Horticultural Science, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, South Korea, Department of Life Sciences, Christ University, Bengaluru, 560029, India; Lee H., Department of Horticultural Science, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, South Korea; Park S.-Y., Department of Horticultural Science, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, South Korea Description An account of the resource Ligusticum officinale is an important medicinal plant belonging to Apiaceae. It does not set seeds and is propagated by rhizome division. However, its sensitivity to high summer temperatures makes field cultivation and genetic resource conservation challenging. To conserve L. officinale germplasm, we employed an in vitro slow-growth storage (SGS) method. Shoot cultures of L. officinale were established on Murashige and Skoog medium supplemented with 1.0 mg/L benzyl adenine, 30 g/L sucrose, and 2.4 g/L gelrite. Cultures were kept for one, three, five, and seven months. The effects of storage temperatures of 25 C (control) versus 15 C, medium supplementation with or without mannitol (3%), and abscisic acid (ABA), 0.5 mg/L, were examined. At the conclusion of the conservation period, survival was measured right away. Four weeks later, the shoot proliferation medium was used to measure the regrowth rate and recovery features. Subsequently, the regenerated shoots were transferred to MS medium supplemented with 1.0 mg/L indole-3-butyric acid for rooting of shoots for 4 weeks. The findings showed that even after seven months, shoot cultures kept at 15 C with medium supplemented with 3% mannitol and 0.5 mg/L ABA maintained a good survival rate of 83.3%. When compared to the control, most growth indices, including shoot length, fresh weight, number of shoots, and number of leaves, were significantly suppressed by mannitol and ABA combined treatment. A regrowth rate of 71% was achieved after transfer to proliferation medium. All the shoots that were cultured on rooting medium involved in rooting and plantlets were successfully acclimatized in controlled conditions. 2026 SAAB. Published by Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies. Subject The topic of the resource In vitroconservation; Ligusticum officinale; Medicinal plant; Shoot proliferation; Slow growth storage Publisher An entity responsible for making the resource available Elsevier B.V. Relation A related resource ISSN: 2546299; CODEN: SAJBD Language A language of the resource English Type The nature or genre of the resource Article Rights Information about rights held in and over the resource Restricted Access; Hardcopy may be available in the library Format The file format, physical medium, or dimensions of the resource online