Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Faculty Publications Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Creator An entity primarily responsible for making the resource Sunil, Sreya; P, Amruth Title A name given to the resource Chemical fingerprinting of Peganum harmala seeds via UVVis, XRD, TGA, FTIR, and GC-MS techniques; In vitro assessment of cytotoxic properties Date A point or period of time associated with an event in the lifecycle of the resource 01-01-2025 Source A related resource from which the described resource is derived Toxicon;Volume;268;Issue;;Article No.;108606; Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.1016/j.toxicon.2025.108606" target="_blank" rel="noreferrer noopener">https://doi.org/10.1016/j.toxicon.2025.108606</a> <br /><br /><a href="https://www.scopus.com/pages/publications/105017703264?origin=resultslist" target="_blank" rel="noreferrer noopener">https://www.scopus.com/pages/publications/105017703264?origin=resultslist</a> Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Sunil S., Department of Life Sciences, Christ University, Karnataka, Bangalore, 560029, India; P A., Department of Life Sciences, Christ University, Karnataka, Bangalore, 560029, India, Centre for Landscapes, Wildlife and Marine Ecology (CLIME), India Description An account of the resource Peganum harmala L., a perennial herb traditionally valued for medicinal and ritual uses, was comprehensively profiled to elucidate its chemical composition and cytotoxic potential. Methanolic seed extracts contained diverse primary and secondary metabolites, including alkaloids, flavonoids, saponins, glycosides, steroids, proteins, and carbohydrates. Proximate analysis revealed high moisture (45.88 %) and crude fibre (18.39 %) with moderate fat (14.74 %) and protein (7.67 %) levels. Spectroscopic studies supported the presence of ?-carboline alkaloids: FTIR spectra showed characteristic functional group vibrations, UVVis displayed a strong absorption at 440 nm, and X-ray diffraction revealed semi-crystalline patterns enriched in harmine and harmaline. GCMS provided definitive chemical identification, detecting harmine (53.13 %) and harmaline (39.12 %) as major constituents. Thermal analyses (TGADTA and DTG) indicated multiphase decomposition typical of complex organic matrices. Cytotoxicity assessment using the MTT assay on L929 fibroblast cells demonstrated a dose-dependent decline in cell viability, with an LC50 of 243.9 ?g/mL, signifying moderatehigh cytotoxic potential. These findings validate the ethnomedicinal significance of P. harmala and underscore its promise for phytomedicine, nutraceutical applications, and pharmaceutical research, while highlighting the necessity of standardized and regulated use to ensure efficacy and safety. 2025 Subject The topic of the resource GC-MS profiling; MTT assay; Phytochemical analysis; Syrian rue seeds; ?-carboline Publisher An entity responsible for making the resource available Elsevier Ltd Relation A related resource ISSN: 410101; CODEN: TOXIA Language A language of the resource English Type The nature or genre of the resource Article Rights Information about rights held in and over the resource All Open Access; Hybrid Gold Open Access Format The file format, physical medium, or dimensions of the resource online