Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Faculty Publications Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Creator An entity primarily responsible for making the resource Gopinath, Shreya; Hosamani, Madhusudan; Basagoudanavar, Suresh; Sreenivasa, B.P.; Joseph, Biljo V.; Patil, Sharanagouda S. Title A name given to the resource Monoclonal antibodies targeting the Erns protein of classical swine fever virus: application for virus detection Date A point or period of time associated with an event in the lifecycle of the resource 01-01-2025 Source A related resource from which the described resource is derived Veterinary Immunology and Immunopathology;Volume;286;Issue;;Article No.;110965; Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.1016/j.vetimm.2025.110965" target="_blank" rel="noreferrer noopener">https://doi.org/10.1016/j.vetimm.2025.110965</a> <br /><br /><a href="https://www.scopus.com/pages/publications/105008568292?origin=resultslist" target="_blank" rel="noreferrer noopener">https://www.scopus.com/pages/publications/105008568292?origin=resultslist</a> Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Gopinath S., CHRIST UNIVERSITY, Karnataka, Bengaluru, India; Hosamani M., ICAR-Indian Veterinary Research Institute, Karnataka, Bengaluru, India; Basagoudanavar S., ICAR-Indian Veterinary Research Institute, Karnataka, Bengaluru, India; Sreenivasa B.P., ICAR-Indian Veterinary Research Institute, Karnataka, Bengaluru, India; Joseph B.V., CHRIST UNIVERSITY, Karnataka, Bengaluru, India; Patil S.S., ICAR-National Institute of Veterinary Epidemiology and Disease Informatics (NIVEDI), Karnataka, Bengaluru, India Description An account of the resource Classical swine fever (CSF), caused by the classical swine fever virus (CSFV; genus Pestivirus), is a highly transmissible disease in pigs that leads to severe economic losses for pig farmers worldwide. Early and accurate diagnosis of CSFV infections is crucial for effective containment of the disease, especially in endemic areas. In this study, the full-length viral protein Erns was successfully expressed in E. coli. The recombinant protein was purified using Ni-NTA affinity chromatography, and its antigenicity confirmed by western blot and indirect ELISA (iELISA) using CSFV-specific antiserum. The purified Erns protein was then used as an immunogen to generate murine hybridomas producing monoclonal antibodies (mAbs). A panel of 15 mAbs targeting this protein was characterized using iELISA and western blotting, for their ability to react with native antigens in cultures infected with CSFV. These mAbs have the potential to be used in the development of diagnostic tools, particularly for the detection and titration of virus-infected cultures through endpoint dilution assays. 2025 Elsevier B.V. Subject The topic of the resource Classical swine fever virus; E^rns ; ELISA; Immunoperoxidase Monoloyer Assay (IPMA); Monoclonal antibodies Publisher An entity responsible for making the resource available Elsevier B.V. Relation A related resource ISSN: 1652427; CODEN: VIIMD Language A language of the resource English Type The nature or genre of the resource Article Rights Information about rights held in and over the resource Restricted Access; Hardcopy may be available in the library Format The file format, physical medium, or dimensions of the resource online