ItemArticle

Laccase-immobilized biochar as a unique host matrix for electrochemical detection of gallic acid: a sustainable engineering approach

Title
Laccase-immobilized biochar as a unique host matrix for electrochemical detection of gallic acid: a sustainable engineering approach
Creator
Thomas, Sona Mariam; Rajeev, Rijo; Sariga; Benny, Libina; Jose, Sandra; Varghese, Anitha
Description
An electrochemical platform for the detection of organic persistent polyphenolic pollutant, gallic acid (GA), was fabricated using an enzyme-immobilization approach over acid-functionalized biochar (f-BC) modified carbon fiber electrode (CFP) electrode. The f-BC was synthesized from dried pineapple leaves and was characterised using X-ray diffraction analysis (XRD), Raman spectroscopy, scanning electron microscopy (SEM), Fourier Transform-Infrared Spectroscopy (FT-IR), and X-ray photoelectron spectroscopy (XPS) for analysing the physicochemical characteristics. The SEM image of Lac/f-BC/CFP confirmed the presence of a porous and granulated surface upon laccase immobilization, while the FTIR spectrum confirmed the presence of C 00000000 00000000 00000000 00000000 11111111 00000000 11111111 00000000 00000000 00000000 O stretch and N-H bend, indicating amide bond formation. The acid treatment of biochar introduced -OH and -COOH groups that further aided in the successful immobilization of laccase via covalent bonding. The fabricated electrode could demonstrate a linear response within the concentration range of 0.012-40 ?M and a low detection limit (LOD) of 9 nM with high selectivity. The fabricated electrode also showcased high practical utility as it could attain high recovery percentage during real sample analysis in tap, pond, sewage and industrial effluent samples. 2025 The Royal Society of Chemistry.
Source
RSC Advances;Volume;15;Issue;33;pp.26886-26896
Date
01-01-2025
Publisher
Royal Society of Chemistry
Coverage
Thomas S.M., Department of Biotechnology, Manipal Institute of Technology, Manipal Academy of Higher Education, Karnataka, Manipal, 576104, India; Rajeev R., Department of Chemistry, Christ University, Karnataka, Bangalore, 560029, India, Centre for Renewable Energy and Environmental Sustainability, Christ University, Karnataka, 560029, India; Sariga, Department of Chemistry, Christ University, Karnataka, Bangalore, 560029, India, Centre for Renewable Energy and Environmental Sustainability, Christ University, Karnataka, 560029, India; Benny L., Department of Chemistry, Christ University, Karnataka, Bangalore, 560029, India, Centre for Renewable Energy and Environmental Sustainability, Christ University, Karnataka, 560029, India; Jose S., Department of Chemistry, Christ University, Karnataka, Bangalore, 560029, India, Centre for Renewable Energy and Environmental Sustainability, Christ University, Karnataka, 560029, India; Varghese A., Department of Chemistry, Christ University, Karnataka, Bangalore, 560029, India, Centre for Renewable Energy and Environmental Sustainability, Christ University, Karnataka, 560029, India
Rights
All Open Access; Gold Open Access
Relation
ISSN: 20462069; CODEN: RSCAC
Format
online
Language
English
Type
Article

Citation

Thomas, Sona Mariam; Rajeev, Rijo; Sariga; Benny, Libina; Jose, Sandra; Varghese, Anitha, “Laccase-immobilized biochar as a unique host matrix for electrochemical detection of gallic acid: a sustainable engineering approach,” CHRIST (Deemed To Be University) Institutional Repository, accessed June 18, 2026, https://archives.christuniversity.in/items/show/22575.

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