ItemArticle

A new benzothiazoloacetonitrile-derived fluorescent probe for selective hydrazine detection and its applications in bioimaging and cotton swab analysis

Title
A new benzothiazoloacetonitrile-derived fluorescent probe for selective hydrazine detection and its applications in bioimaging and cotton swab analysis
Creator
Durgi, Nikita Kush; D. K., Ramesh; Manjappa, Kiran B.; Pujar, Prasad Pralhad
Description
Hydrazine (N2H4) is extensively utilized in various chemical industries. However, it is a highly toxic and explosive chemical posing a serious risk to human health and the environment, which warrants its quick and selective detection. To address this issue, we introduce a benzothiazoloacetonitrile-based fluorescent probe containing a recognition site for hydrazine detection. Adding the benzothiazoloacetonitrile group to the phenanthroline-based imidazole fluorophore increased BTN's electrophilicity, aiding the nucleophilic attack by hydrazine. This led to a rapid fluorescence change from orange to green within one minute, with a limit of detection (LOD) of 0.21 M, resulting from the cleavage of the olefinic bond between the donor and acceptor units. The probe's selective response to hydrazine was supported by a specific reaction mechanism, confirmed by LC-MS and DFT studies. Additionally, the probe can detect hydrazine using cotton swabs for quick, on-site testing. It also allows for clear visualisation in living cells through different fluorescence channels. Overall, these results demonstrate that the probe exhibits significant potential for the detection of hydrazine in environmental and biological samples. This journal is The Royal Society of Chemistry, 2026.
Source
Analyst ;
Date
01-01-2026
Publisher
Royal Society of Chemistry
Coverage
Durgi N.K., Department of Chemistry, Christ University, Karnataka, Bangalore, 560029, India, Centre for Renewable Energy and Environmental Sustainability, Christ University, Bangalore, 560029, India; D. K. R., Department of Chemistry, Christ University, Karnataka, Bangalore, 560029, India, Centre for Renewable Energy and Environmental Sustainability, Christ University, Bangalore, 560029, India; Manjappa K.B., College of Science, Tunghai University, Bangalore, 560029, India; Pujar P.P., Department of Chemistry, Christ University, Karnataka, Bangalore, 560029, India, Centre for Renewable Energy and Environmental Sustainability, Christ University, Bangalore, 560029, India
Rights
Restricted Access; Hardcopy may be available in the library
Relation
ISSN: 32654; CODEN: ANALA
Format
online
Language
English
Type
Article

Citation

Durgi, Nikita Kush; D. K., Ramesh; Manjappa, Kiran B.; Pujar, Prasad Pralhad, “A new benzothiazoloacetonitrile-derived fluorescent probe for selective hydrazine detection and its applications in bioimaging and cotton swab analysis,” CHRIST (Deemed To Be University) Institutional Repository, accessed June 18, 2026, https://archives.christuniversity.in/items/show/22601.

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