Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Faculty Publications Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Creator An entity primarily responsible for making the resource Harilal, Calvin Carl; Uwaya, Gloria Ebube; Kanchi, Suvardhan; Bisetty, Krishna Title A name given to the resource Engineered MOF-199 Modified Electrodes for Enhanced Electrochemical Immunosensing of Lactoferrin via Signal Amplification Date A point or period of time associated with an event in the lifecycle of the resource 01-01-2025 Source A related resource from which the described resource is derived Journal of the Electrochemical Society;Volume;172;Issue;12;Article No.;126503; Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.1149/1945-7111/ae2bac" target="_blank" rel="noreferrer noopener">https://doi.org/10.1149/1945-7111/ae2bac</a> <br /><br /><a href="https://www.scopus.com/pages/publications/105033935861?origin=resultslist" target="_blank" rel="noreferrer noopener">https://www.scopus.com/pages/publications/105033935861?origin=resultslist</a> Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Harilal C.C., Department of Chemistry, Faculty of Applied Sciences, Durban University of Technology, Durban, 4000, South Africa; Uwaya G.E., Department of Chemistry, Faculty of Applied Sciences, Durban University of Technology, Durban, 4000, South Africa; Kanchi S., Department of Chemistry, Bengaluru, 560029, India, Centre for Renewable Energy and Environmental Sustainability, CHRIST University, Bengaluru, 560 029, India; Bisetty K., Department of Chemistry, Faculty of Applied Sciences, Durban University of Technology, Durban, 4000, South Africa Description An account of the resource The detection of lactoferrin (LF), an essential immunological and nutritional biomarker, demands highly sensitive analytical platforms for low-level detection. In this study, a label-free electrochemical immunosensing platform was constructed by immobilizing anti-lactoferrin antibodies (Anti-LF-Ab) on a multiwalled carbon nanotube and a metalorganic framework (MWCNT/MOF-199) nanocomposite-modified glassy carbon electrode (GCE). The synergy between MWCNTs and MOF-199 provided abundant active sites for antibody immobilization and enhanced electron transfer, yielding an eightfold current increase compared to bare GCE. Electrochemical analyses confirmed efficient charge transfer and stable antibody binding. Under optimized conditions, the immunosensor exhibited exceptional analytical performance with a low detection limit of 5.24 ng ml?1 and a quantification limit of 17.46 ng ml?1 across a wide detection range of 050 ng ml?1. The platform demonstrated strong analytical reliability, including excellent repeatability (RSD &lt; 5%), reproducibility and operational stability over multiple measurement cycles for LF detection in food diagnostics. In addition, Monte Carlo simulations confirmed the stability of the layer-by-layer assembly, supporting the robustness of the engineered sensing interface. 2025 The Author(s). Published on behalf of The Electrochemical Society by IOP Publishing Limited. Subject The topic of the resource electrochemical immunosensor; lactoferrin; metal-organic frameworks Publisher An entity responsible for making the resource available Institute of Physics Relation A related resource ISSN: 134651; CODEN: JESOA Language A language of the resource English Type The nature or genre of the resource Article Rights Information about rights held in and over the resource All Open Access; Hybrid Gold Open Access Format The file format, physical medium, or dimensions of the resource online