Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Faculty Publications Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Creator An entity primarily responsible for making the resource Kim, Ye-Won; Lee, Han-Sol; Murthy, Hosakatte Niranjana; Lee, Hyoshin; Park, So-Young Title A name given to the resource An in vitro slow-growth callus conservation strategy for several medicinal plants using response surface methodology and machine learning Date A point or period of time associated with an event in the lifecycle of the resource 01-01-2026 Source A related resource from which the described resource is derived BMC Biotechnology;Volume;26;Issue;1;Article No.;49; Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.1186/s12896-026-01129-x" target="_blank" rel="noreferrer noopener">https://doi.org/10.1186/s12896-026-01129-x</a> <br /><br /><a href="https://www.scopus.com/pages/publications/105036335427?origin=resultslist" target="_blank" rel="noreferrer noopener">https://www.scopus.com/pages/publications/105036335427?origin=resultslist</a> Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Kim Y.-W., Department of Horticultural Science, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, South Korea; Lee H.-S., Department of Horticultural Science, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, South Korea; Murthy H.N., Department of Horticultural Science, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, South Korea, Department of Life Sciences, Christ University, Bengaluru, 560029, India; Lee H., Department of Horticultural Science, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, South Korea; Park S.-Y., Department of Horticultural Science, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, South Korea Description An account of the resource Background: In vitro culture of callus is an effective method for conserving the genetic resources of economically important crops. However, continuous subculturing is a costly and labor-intensive process. Therefore, establishing an efficient in vitro long-term conservation system applicable to various plant species is required. In this study, calli derived from five medicinal plant species, Camellia japonica (Cj), Centella asiatica (Ca), Ligusticum afficinale (Lo), Panax ginseng (Pg), and Sageratia thea (St) were used to optimize storage conditions and establish a suitable in vitro conservation strategy. Calli cultures were maintained on the appropriate culture medium at 5C for 120 days. Cell viability and regrowth rate were assessed during the storage period, and correlations between growth and antioxidant traits were examined. Subsequently, pretreatment optimization using sucrose (39%), MeJA (0-200 M), and CTR (020mg/L) was performed using RSM, and the effects of pretreatment and storage temperature on callus conservation were evaluated. In addition, machine learning models such as GRNN, MLP, RF, SVR, and XGBoost were applied to the experimental data. Results: The findings demonstrated that, in comparison to Ca and St, Lo, Pg, and Cj exhibited noticeably higher antioxidant capacity while maintaining high cell viability and regrowth rates. Interestingly, Ca and St drastically decreased viability and regrowth after 60 days, whereas Lo, Pg, and Cj maintained viability and regeneration for up to 90 days. Both TPC and AC (measured by FRAP assay) showed a high positive correlation with cell viability and growth rate, according to correlation analysis. RSM predicted that the optimal pretreatment medium for enhancing antioxidant capacity was the species-specific proliferation medium supplemented with 3% sucrose, 135 M MeJA, and 20mg/L CTR, while the highest TSSC was achieved using the species-specific proliferation medium supplemented with 9% sucrose and 200 M MeJA. When the storage temperature was set to 15C following the antioxidant-enhancing pretreatment derived from the RSM optimization, all five species showed improved cell viability and regrowth rates, among the storage methods. Among the ML models tested, XGBoost demonstrated the most stable and accurate predictive performance for both viability and regrowth during in vitro conservation. SHAP-based analysis of the XGBoost model, focusing on regrowth rate, revealed that storage duration was the most influential factor affecting regrowth prediction, followed by storage temperature, while pretreatment conditions showed secondary but meaningful contributions. Conclusions: This study demonstrates that long-term callus conservation is closely associated with AC and TPC. Medium supplemented with sucrose 3%, 135 M MeJA, and 20mg/L CTR, followed by storage at 15C, significantly improved viability and regrowth, and calli could be maintained up to 120 days without subculturing. This approach provides an efficient and broadly applicable in vitro strategy for the conservation of diverse plant genetic resources. The Author(s) 2026. Subject The topic of the resource Antioxidant capacity; Citric acid; In vitro conservation; Methyl jasmonate; Plant cell culture; Response surface methodology Publisher An entity responsible for making the resource available BioMed Central Ltd Relation A related resource ISSN: 14726750; CODEN: BBMIE Language A language of the resource English Type The nature or genre of the resource Article Rights Information about rights held in and over the resource All Open Access; Gold Open Access; Green Open Access Format The file format, physical medium, or dimensions of the resource online