Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Faculty Publications Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Creator An entity primarily responsible for making the resource Dhasaradhan, Amrutha Pannikassery; Paari, Kuppusamy Alagesan Title A name given to the resource Toxicological evaluation of Calotropis gigantea (L.) W. T. Aiton (Apocynaceae) stem extract in zebrafish: A chronic exposure study Date A point or period of time associated with an event in the lifecycle of the resource 01-01-2025 Source A related resource from which the described resource is derived Plant Science Today;Volume;12;Issue;3;pp.1-12 Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.14719/pst.9323" target="_blank" rel="noreferrer noopener">https://doi.org/10.14719/pst.9323</a> <br /><br /><a href="https://www.scopus.com/pages/publications/105017616715?origin=resultslist" target="_blank" rel="noreferrer noopener">https://www.scopus.com/pages/publications/105017616715?origin=resultslist</a> Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Dhasaradhan A.P., Department of Life Sciences, Christ University, Karnataka, Bangalore, 560 029, India; Paari K.A., Department of Life Sciences, Christ University, Karnataka, Bangalore, 560 029, India Description An account of the resource Calotropis gigantea is widely used in traditional medicine across rural and tribal regions for treating various ailments. The safety profile of this plant especially in concerning long term or high dose exposure, remains inadequately studied. This study aims to scientifically assess the toxicity of C. gigantea stem extract using zebrafish (Danio rerio) as a model organism. Chronic exposure over 10, 20 and 30 days revealed significant oxidative stress, mitochondrial impairment and histopathological alterations in vital organs. Key antioxidant enzymes glutathione reductase (GR), glutathione S-transferase (GST), succinate dehydrogenase (SDH), catalase (CAT) and superoxide dismutase (SOD) were analysed in the liver, gills, brain and muscles. Enzyme activity has initially increased but declined by the 30th day, indicating progressive oxidative damage. The liver and gills exhibited the most substantial biochemical and structural changes. The histological analysis confirmed cellular degeneration, inflammation and necrosis. These findings highlight the potential risks associated with unregulated therapeutic use of C. gigantea and emphasize the need for scientific validation and public awareness to ensure safe application. The Author(s). This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited (https://creativecommons.org/licenses/by/4.0/) Subject The topic of the resource histopathological alterations; oxidative stress; stress induced damage; zebrafish toxicity Publisher An entity responsible for making the resource available Horizon e-Publishing Group Relation A related resource ISSN: 23481900; Language A language of the resource English Type The nature or genre of the resource Article Rights Information about rights held in and over the resource All Open Access; Gold Open Access; Green Open Access Format The file format, physical medium, or dimensions of the resource online