Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Faculty Publications Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Creator An entity primarily responsible for making the resource Rithu, K.; Gayathri, Jayasankar; Nag, Anish Title A name given to the resource Development of a Thin Layer Chromatography method to detect the presence of spiked Steroid drugs in Herbal Crude extracts Date A point or period of time associated with an event in the lifecycle of the resource 01-01-2026 Source A related resource from which the described resource is derived Research Journal of Chemistry and Environment;Volume;30;Issue;2;pp.90-97 Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.25303/302rjce090097" target="_blank" rel="noreferrer noopener">https://doi.org/10.25303/302rjce090097</a> <br /><br /><a href="https://www.scopus.com/pages/publications/105033092080?origin=resultslist" target="_blank" rel="noreferrer noopener">https://www.scopus.com/pages/publications/105033092080?origin=resultslist</a> Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Rithu K., Department of Life Sciences, Christ University, Karnataka, Bangalore, 560029, India; Gayathri J., Department of Life Sciences, Christ University, Karnataka, Bangalore, 560029, India; Nag A., Department of Life Sciences, Christ University, Karnataka, Bangalore, 560029, India Description An account of the resource This study aimed to develop a better, faster and more efficient TLC method for detecting steroids in the herbal samples. The steroid drugs dexamethasone and prednisolone were considered possible adulterants and were spiked into herbal extracts of neem and amla. Optimization of the TLC mobile phases for better resolution for prominent visualisation of spots was performed with several combinations of solventsnamely, methanol, chloroform, ethyl acetate, toluene, ethanol, isopropyl alcohol, acetic acid and petroleum ether and, spray reagents such as iodine, potassium permanganate, sulphuric acid and, methanol. The optimal result was obtained using chloroform and methanol (9:1, v/v) as the mobile phase and methanol-sulphuric acid (9:1, v/v) as the spraying agent. The method was also able to separate a mixture of dexamethasone and prednisolone in the ratio of 1:1. The retention factors (Rf) for the steroids dexamethasone and prednisolone were within the range of 0.50-0.55 and 0.73-0.83 respectively. The lowest detection limit for the steroid drugs when mixed with herbal samples (neem and amla) was 0.1 g/ml. The developed TLC method is robust and can be conveniently utilised for detecting steroid adulterants in herbal samples. 2026, World Researchers Associations. All rights reserved. Subject The topic of the resource Adulteration; dexamethasone; herbal medicines; prednisolone; steroids; thin layer chromatography Publisher An entity responsible for making the resource available World Researchers Associations Relation A related resource ISSN: 9720626; Language A language of the resource English Type The nature or genre of the resource Article Rights Information about rights held in and over the resource Restricted Access; Hardcopy may be available in the library Format The file format, physical medium, or dimensions of the resource online