Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Faculty Publications Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Creator An entity primarily responsible for making the resource Jiang, Yingying; Alagesan, Paari Kuppusamy Title A name given to the resource Biosynthesis of silver nanoparticles using Vitex negundo: Pesticidal efficacy against Spodoptera litura and Helicoverpa armigera with a safety assessment Date A point or period of time associated with an event in the lifecycle of the resource 01-01-2025 Source A related resource from which the described resource is derived Journal of Applied and Natural Science;Volume;17;Issue;3;pp.1180-1192 Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.31018/jans.v17i3.6708" target="_blank" rel="noreferrer noopener">https://doi.org/10.31018/jans.v17i3.6708</a> <br /><br /><a href="https://www.scopus.com/pages/publications/105017457060?origin=resultslist" target="_blank" rel="noreferrer noopener">https://www.scopus.com/pages/publications/105017457060?origin=resultslist</a> Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Jiang Y., Department of Life Sciences, CHRIST (Deemed to be University), Bangalore, India; Alagesan P.K., Department of Life Sciences, CHRIST (Deemed to be University), Bangalore, India Description An account of the resource Plant-based metallic nanoparticles hold promise for agricultural applications. This study investigated the biosynthesis of silver nanoparticles (AgNPs) using Vitex negundo leaf extract. It assessed their pesticidal properties and safety. AgNPs were synthesized via aqueous extract and characterized by UVVis spectroscopy, dynamic light scattering (DLS), Scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR) Characterization revealed a surface plasmon resonance (SPR) peak at 435 nm, an average particle size of 236.4 nm, and a stable zeta potential. SEM confirmed spherical morphology, XRD indicated a face-centred cubic (fcc) structure, and FTIR confirmed biomolecule-mediated capping and stabilization. Larvicidal bioassays showed that 5 mg/L AgNPs caused 78-89% mortality in Spodoptera litura and 83-100% in Helicoverpa armigera. In both pests, oxidative stress was triggered. Catalase (CAT) activity increased by over 80% in S.litura and moderately in H. armigera. Glutathione-S-transferase (GST) activity declined post-treatment, from 9.4 to 5.0 mM/ min/mg protein in H. armigera and from 1.0 to 0.7 mM/min/mg protein in S. litura, indicating impaired detoxification. In Oreo-chromis urolepis, AgNPs (5, 10, and 25 mg/L) induced oxidative stress without mortality. CAT activity decreased within 24 hours (72.4% at 5 mg/L) and remained suppressed by day 5 (36.7% at 25 mg/L). Superoxide dismutase (SOD) activity increased at 5 mg/L (19.8%) and 25 mg/L (54.8%), while GST initially declined but rebounded at higher concentrations. Reduced glutathione (GSH) levels increased by 24.3% at 25 mg/L, indicating adaptation. This study highlights that V. negundo-derived AgNPs are effective, eco-friendly biopesticides with minimal non-target toxicity. Author (s). Subject The topic of the resource Characterization; Larvicidal activity; Safety assessment; Silver nanoparticles; Vitex negundo leaves Publisher An entity responsible for making the resource available Applied and Natural Science Foundation Relation A related resource ISSN: 9749411; Language A language of the resource English Type The nature or genre of the resource Article Rights Information about rights held in and over the resource All Open Access; Gold Open Access; Green Open Access Format The file format, physical medium, or dimensions of the resource online