Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Faculty Publications Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Creator An entity primarily responsible for making the resource Ganavi, B.B.; Paari, Kuppusamy Alagesan Title A name given to the resource Evaluation of Potential Toxicity of Calotropis gigantea Leaf Extract on Danio rerio: Bioactive Compounds, Oxidative Stress and Histological Toxicity Analysis Date A point or period of time associated with an event in the lifecycle of the resource 01-01-2025 Source A related resource from which the described resource is derived International Journal of Advancement in Life Sciences Research;Volume;8;Issue;4;pp.111-127 Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.31632/ijalsr.2025.v08i04.009" target="_blank" rel="noreferrer noopener">https://doi.org/10.31632/ijalsr.2025.v08i04.009</a> <br /><br /><a href="https://www.scopus.com/pages/publications/105023296898?origin=resultslist" target="_blank" rel="noreferrer noopener">https://www.scopus.com/pages/publications/105023296898?origin=resultslist</a> Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Ganavi B.B., Department of Life Sciences, CHRIST University, Hosur Road, Bengaluru, 560029, India; Paari K.A., Department of Life Sciences, CHRIST University, Hosur Road, Bengaluru, 560029, India Description An account of the resource A greater understanding of the ecotoxicological effects of plant-based products is required due to their increasing use. Despite its long-recognised medicinal properties, Calotropis gigantea possesses toxic characteristics that necessitate further investigation into its chemical composition and biological effects. This study evaluates the toxicity of crude C. gigantea leaf extracts on the freshwater fish Danio rerio over a 30-day exposure period. Alongside GCMS analysis, which identified 48 major constituents, the total phenolic content (TPC), total flavonoid content (TFC), and antioxidant activity were assessed using the DPPH assay. The extracts exhibited a higher flavonoid content than phenolic content, indicating strong antioxidant potential. In contrast, biochemical analyses revealed that exposed fish showed significantly elevated levels of oxidative stress biomarkers, including succinate dehydrogenase (SDH), glutathione S-transferase (GST), glutathione reductase (GR), catalase (CAT), and superoxide dismutase (SOD), signifying cellular stress and disruption of redox homeostasis. Histological examinations of the liver, brain, muscle, and gills of treated fish demonstrated notable alterations. The gills exhibited lamellar fusion and epithelial lifting, impairing respiration. The brain showed neuronal vacuolation and degeneration, suggesting neurotoxicity, while muscle tissues displayed myofibrillar degeneration. Liver samples revealed hepatocyte necrosis and vacuolisation, indicative of metabolic dysfunction. Collectively, these enzymatic and systemic histopathological responses emphasise the toxicological impacts of C. gigantea extracts on aquatic organisms. The findings underscore the importance of ecotoxicological evaluations and the dual nature of medicinal plants such as C. gigantea, which can be both therapeutic and harmful. In addition to stressing the necessity for cautious application and further research to elucidate the mechanisms underlying their toxicity, this study provides valuable insights into the biological effects of plant-derived compounds. 2025, Dr Tarak Nath Podder Memorial Foundation. All rights reserved. Subject The topic of the resource Calotropis gigantea; Histology; Oxidative Stress; Phytochemical Analysis; Zebrafish Publisher An entity responsible for making the resource available Dr Tarak Nath Podder Memorial Foundation Relation A related resource ISSN: 25814877; Language A language of the resource English Type The nature or genre of the resource Article Rights Information about rights held in and over the resource All Open Access; Gold Open Access Format The file format, physical medium, or dimensions of the resource online