Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Faculty Publications Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Creator An entity primarily responsible for making the resource Shinde, Kajal Sunil; Jangme, Chandraprabhu Motichand; Patil, Abhinandan Raosaheb Title A name given to the resource RP-HPLC METHOD FOR QUANTITATIVE ESTIMATION OF NAFTIFINE HYDROCHLORIDE IN FORMULATED PRODUCTS: DEVELOPMENT AND VALIDATION Date A point or period of time associated with an event in the lifecycle of the resource 01-01-2025 Source A related resource from which the described resource is derived Journal of Applied Pharmaceutical Research;Volume;13;Issue;4;pp.177-186 Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.69857/joapr.v13i4.1115" target="_blank" rel="noreferrer noopener">https://doi.org/10.69857/joapr.v13i4.1115</a> <br /><br /><a href="https://www.scopus.com/pages/publications/105016659048?origin=resultslist" target="_blank" rel="noreferrer noopener">https://www.scopus.com/pages/publications/105016659048?origin=resultslist</a> Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Shinde K.S., Dept. of Pharmaceutical Science, Centre for Interdisciplinary Research, D. Y. Patil Education Society (Deemed to be University), Maharashtra, Kolhapur, 416006, India; Jangme C.M., Department of Pharmacology, D. Y. Patil College of Pharmacy, D. Y. Patil Education Society, Deemed to Be University, Maharashtra, Kolhapur, 416006, India; Patil A.R., Department of Pharmaceutics, D. Y. Patil College of Pharmacy, D. Y. Patil Education Society, Deemed to Be University, Kolhapur, Maharashtra, 416006, India Description An account of the resource Background: Naftifine hydrochloride is an allylamine antifungal agent commonly used to treat dermatophyte infections. It inhibits squalene epoxidase, a key enzyme in ergosterol biosynthesis, thereby disrupting the integrity of the fungal cell membrane. It exhibits broad-spectrum activity against dermatophytes, yeasts, and molds, and is typically formulated as a 1% topical cream or gel. Methodology: A rapid and robust reverse-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for the estimation of naftifine hydrochloride in a topical cream formulation (2% Naftifast, Zydus), in accordance with ICH and FDA guidelines. Chromatographic separation was achieved on an Inertsil ODS column using an isocratic mobile phase consisting of 35% acetonitrile, 40% methanol, 25% water, and 0.8% triethylamine (pH adjusted to 5.5 with acetic acid) at a flow rate of 1.4 mL/min. Detection was performed at 265 nm. Results and Discussion: Naftifine hydrochloride showed a retention time of approximately 4.0 minutes with a total run time of 6.0 minutes. The method displayed excellent linearity over a concentration range of 20120 g/mL (R &gt; 0.999). Recovery studies indicated a mean recovery of 100.4%. Precision was confirmed by relative standard deviation (RSD) values of less than 2%, demonstrating the methods reproducibility. Conclusion: The proposed RP-HPLC method is simple, precise, and time-efficient. It is suitable for routine quality control of naftifine hydrochloride in pharmaceutical dosage forms due to its short analysis time and strong validation performance. 2025 The authors. Subject The topic of the resource Naftifine hydrochloride; pharmaceutical; reversed-phase HPLC; topical antifungal; validation Publisher An entity responsible for making the resource available Creative Pharma Assent Relation A related resource ISSN: 23480335; Language A language of the resource English Type The nature or genre of the resource Article Rights Information about rights held in and over the resource All Open Access; Gold Open Access; Green Open Access Format The file format, physical medium, or dimensions of the resource online