Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Faculty Publications Article Faculty Publications -Articles Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Creator An entity primarily responsible for making the resource Koujalagi, Koushik; Malaviya, Alok Kumar Title A name given to the resource Screening, isolation, characterization, and optimization of BSH activity from potential probiotic isolates from various sources Date A point or period of time associated with an event in the lifecycle of the resource 01-01-2025 Source A related resource from which the described resource is derived Journal of Applied Biology and Biotechnology;Volume;13;pp.86-96 Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.7324/JABB.2025.224557" target="_blank" rel="noreferrer noopener">https://doi.org/10.7324/JABB.2025.224557</a> <br /><br /><a href="https://www.scopus.com/pages/publications/105011975466?origin=resultslist" target="_blank" rel="noreferrer noopener">https://www.scopus.com/pages/publications/105011975466?origin=resultslist</a> Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Koujalagi K., Applied and Industrial Biotechnology Laboratory, Department of Life Sciences, Christ (Deemed to be University), Bengaluru, India; Malaviya A.K., Applied and Industrial Biotechnology Laboratory, Department of Life Sciences, Christ (Deemed to be University), Bengaluru, India Description An account of the resource Bile salt hydrolase (BSH)-producing probiotics can assimilate cholesterol from the body through de novo synthesis. The BSH enzyme was found in 23 of 513 isolates accessed from various sources. Five of the 23 BSH-positive strains have been selected for further study, based on their BSH activity, compared to two positive controls, Lactobacillus acidophilus and Enterococcus lactis. The Grams nature of the strains was determined and further examined for hemolytic activity, gelatinase, and catalase assay as per Indian Council for Medical ResearchDepartment of Biotechnology recommendations. Two Enterococcus faecalis (CGz3 and CGz4) strains with ?-hemolytic, negative catalase, and gelatinase activity are selected for probiotic characterization, evaluating the organisms surface hydrophobicity, autoaggregation tests, tolerance to lysozyme, gastric acidity, bile salt and gastric juices (pepsin and pancreatin). The strain which withstands the harsh gastrointestinal conditions was considered for further experiments. To establish a standardized method to quantify the BSH activity of the potential probiotic isolate, substrate utilization was performed by screening sodium glycocholate (GCA) and taurocholic acid (TCA) at different concentrations. The optimal BSH activity was observed at the 16th hour and 0.1% (v/v) GCA. Based on the standardized protocol, factorial optimization of process parameters, such as pH, inoculum percentage, temperature, and revolutions per minute (RPM) was carried out for increased BSH activity. The optimal BSH activity was observed at pH 5.5 and 1% inoculum (v/v). The highest BSH activity was obtained at 40C and 200 RPM. Among the other BSH-positive strains, E. faecalis CGz3 shows the best probiotic potential. The strain would be further studied for its ability to alleviate symptoms associated with non-alcoholic fatty liver disease (NAFLD), using a cell line-based study and associated gene regulation. In conclusion, E. faecalis CGz3 would have the potential to be used as a dietary supplement to treat metabolic disorders, such as hypercholesterolemia and NAFLD/metabolic-associated fatty liver disease. 2025 Koushik Koujalagi and Alok Kumar Malaviya. Subject The topic of the resource and thin-layer chromatography (TLC); Bile salt hydrolase; cholesterol assimilation; probiotics; process parameter optimization Publisher An entity responsible for making the resource available Open Science Publishers LLP Inc. Relation A related resource ISSN: 2347212X; Language A language of the resource English Type The nature or genre of the resource Article Rights Information about rights held in and over the resource All Open Access; Gold Open Access Format The file format, physical medium, or dimensions of the resource online