Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Title A name given to the resource Faculty Publications Book Chapter Faculty Publications- Book Chapter Dublin Core The Dublin Core metadata element set is common to all Omeka records, including items, files, and collections. For more information see, http://dublincore.org/documents/dces/. Creator An entity primarily responsible for making the resource Chivukula, Jahnavi; Ponnachan, Blessy; Kumar, Dipanshu; Nagella, Praveen Title A name given to the resource Structure and mechanism of zinc-finger nucleases-mediated genome editing in plants Date A point or period of time associated with an event in the lifecycle of the resource 01-01-2025 Source A related resource from which the described resource is derived Genome Editing for Crop Improvement: Theory and Methodology;pp.35-46 Identifier An unambiguous reference to the resource within a given context <a href="https://doi.org/10.1079/9781800622517.0003" target="_blank" rel="noreferrer noopener">https://doi.org/10.1079/9781800622517.0003</a> <br /><br /><a href="https://www.scopus.com/pages/publications/105003982530?origin=resultslist" target="_blank" rel="noreferrer noopener">https://www.scopus.com/pages/publications/105003982530?origin=resultslist</a> Coverage The spatial or temporal topic of the resource, the spatial applicability of the resource, or the jurisdiction under which the resource is relevant Chivukula J., Department of Life Sciences, School of Sciences, CHRIST (Deemed to be University), Bangalore, India; Ponnachan B., Department of Life Sciences, School of Sciences, CHRIST (Deemed to be University), Bangalore, India; Kumar D., Department of Life Sciences, School of Sciences, CHRIST (Deemed to be University), Bangalore, India; Nagella P., Department of Life Sciences, School of Sciences, CHRIST (Deemed to be University), Bangalore, India Description An account of the resource The chapter provides a comprehensive exploration of zinc-finger nucleases (ZFNs), intricate molecular tools designed to integrate genes of interest into target sites, thereby instigating both phenotypic and genotypic transformations. The focal point of this genetic manipulation lies in the activity of the FokI endonuclease enzyme, which, through the formation of homodimers, orchestrates precise cleavage at integration sites. Leveraging the wealth of genomic information across diverse organisms, the chapter elucidates the mechanistic underpinnings of ZFNs. A particular emphasis is placed on the modular assembly of ZFNs, unravelling the formation of ??? motifs and explicating the nuanced mechanisms governing their actions. The application of this methodology in plant engineering holds paramount significance, particularly in the realm of augmenting stress tolerance and nutritional value. The chapter systematically examines the gene of interest for various plants, including tobacco, soybean, Arabidopsis thaliana, rice, and wheat, elucidating the corresponding ZFN mechanisms. The amalgamation of sophisticated genetic tools with a detailed understanding of plant genomes presents a promising avenue for tailoring crops to meet diverse agricultural challenges. This work not only contributes to the fundamental understanding of ZFNs but also underscores their practical implications in advancing crop improvement strategies for sustainable agriculture. CAB International 2025. All rights reserved. Subject The topic of the resource Crop plants; Endonuclease enzyme; Genome editing; Zinc-finger nucleases Publisher An entity responsible for making the resource available CABI International Relation A related resource ISBN: 978-180062250-0; 978-180062249-4; Language A language of the resource English Type The nature or genre of the resource Book chapter Rights Information about rights held in and over the resource Restricted Access; Hardcopy may be available in the library Format The file format, physical medium, or dimensions of the resource online