Structure and mechanism of zinc-finger nucleases-mediated genome editing in plants
subject: Crop plants; Endonuclease enzyme; Genome editing; Zinc-finger nucleases
description: The chapter provides a comprehensive exploration of zinc-finger nucleases (ZFNs), intricate molecular tools designed to integrate genes of interest into target sites, thereby instigating both phenotypic and genotypic transformations. The focal point of this genetic manipulation lies in the activity of the FokI endonuclease enzyme, which, through the formation of homodimers, orchestrates precise cleavage at integration sites. Leveraging the wealth of genomic information across diverse organisms, the chapter elucidates the mechanistic underpinnings of ZFNs. A particular emphasis is placed on the modular assembly of ZFNs, unravelling the formation of ??? motifs and explicating the nuanced mechanisms governing their actions. The application of this methodology in plant engineering holds paramount significance, particularly in the realm of augmenting stress tolerance and nutritional value. The chapter systematically examines the gene of interest for various plants, including tobacco, soybean, Arabidopsis thaliana, rice, and wheat, elucidating the corresponding ZFN mechanisms. The amalgamation of sophisticated genetic tools with a detailed understanding of plant genomes presents a promising avenue for tailoring crops to meet diverse agricultural challenges. This work not only contributes to the fundamental understanding of ZFNs but also underscores their practical implications in advancing crop improvement strategies for sustainable agriculture. CAB International 2025. All rights reserved.
creator: Chivukula, Jahnavi; Ponnachan, Blessy; Kumar, Dipanshu; Nagella, Praveen
source: Genome Editing for Crop Improvement: Theory and Methodology;pp.35-46
publisher: CABI International
date: 01-01-2025
rights: Restricted Access; Hardcopy may be available in the library
relation: ISBN: 978-180062250-0; 978-180062249-4;
language: English
type: Book chapter
coverage: Chivukula J., Department of Life Sciences, School of Sciences, CHRIST (Deemed to be University), Bangalore, India; Ponnachan B., Department of Life Sciences, School of Sciences, CHRIST (Deemed to be University), Bangalore, India; Kumar D., Department of Life Sciences, School of Sciences, CHRIST (Deemed to be University), Bangalore, India; Nagella P., Department of Life Sciences, School of Sciences, CHRIST (Deemed to be University), Bangalore, India