A Comprehensive Study on Parametric Optimization of Plasma-Sprayed Cr2C3 Coatings on Al6061 Alloy
AA6061 aluminum alloy substrate; Analysis of variance; Bond strength; Cr2C3 coatings; Design of experiments; Fracture analysis; Microhardness; Plasma spray; Porosity
Plasma spray, a widely employed thermal spray method, is known for enhancing coatings with heightened microhardness, density, and bonding strength. In this study, Taguchis approach was applied to optimize processing parameters for plasma spray-coated surfaces, aiming to reduce porosity, increase hardness, and fortify the connection between Cr2C3 coatings. The design of experiments method facilitated the optimization of process parameters, utilizing signal-to-noise ratios and ANOVA analysis to assess the significance of each processing parameter and identify optimal parameter combinations. Powdered feed rate and stand-off distance emerged as the two most critical processing variables influencing permeability and hardness, contingent on signal-to-noise ratios. S/N ratio analysis was employed to determine the optimal processing parameters for permeability, hardness, and bonding strength. For porosity, the optimal stand-off distance, powdered feed rate, and current density were identified as 60rpm, 50g/min, and 460ampsmm/s, respectively. Exemplary process conditions for hardness included a powdered feed rate of 60g/min, a stand-off distance of 80rpm, and a current density of 480 amps. Lastly, for strength properties, the ideal process variables were a stand-off distance of 80rpm, a current density of 480amps, and a powdered feed rate of 60g/min. Despite small differences between projected R2 and modified R2 values in statistical data on permeability, hardness, and bonding strength, the proximity to the one emphasizing the fit of the linear regression used for analysis was evident. Fracture results from the binding strength test postulate mixed adhesion-cohesion type failures in the Cr2C3 coatings. The Institution of Engineers (India) 2024.
Kumar, G. S. Pradeep; Keshavamurthy, R.; Santosh, M. Siva; Reddy, Siddavatam Narayana; Tambrallimath, Vijay
Journal of The Institution of Engineers (India): Series D;Volume;106;Issue;3;pp.1393-1408
Restricted Access; Hardcopy may be available in the library
https://doi.org/10.1007/s40033-024-00692-x https://www.scopus.com/pages/publications/85188444673?origin=resultslist
Kumar G.S.P., Department of Automobile Engineering, CHRIST University, Bengaluru, 560074, India; Keshavamurthy R., Department of Mechanical Engineering, Dayananda Sagar College of Engineering, Bengaluru, 560078, India; Santosh M.S., Department of Automobile Engineering, CHRIST University, Bengaluru, 560074, India; Reddy S.N., Department of Automobile Engineering, CHRIST University, Bengaluru, 560074, India; Tambrallimath V., Department of Automobile Engineering, Dayananda Sagar College of Engineering, Bengaluru, 560078, India
Machining Characteristics Evaluation of Al7075TiB2 In Situ Composite Using Abrasive Water Jet Machining with Varied Test Parameters
Abrasive water jet; Al7075TiB2; In situ process; Surface roughness; VMRR
The study delves into the abrasive water jet (AWJ) cutting of an Al7075TiB2 metal matrix composite that was synthesized in situ. The primary goal is to investigate how variations in three key process parameters, namely, stand-off distance (SOD) ranging from 0.5 to 2.5 mm, abrasive flow rate (100 to 300 g min), and traverse speed (100 to 500 mm min), affect three critical performance metrics: volumetric material removal rate (VMRR), dimensional accuracy, and surface roughness (SR). The studys findings were represented graphically, highlighting the relationships between these responses and the aforementioned process parameters. Scanning electron microscopy (SEM) was also used to examine the machined surfaces. It was discovered that increasing traverse speed resulted in significant increases in surface roughness, VMRR, and dimensional errors. An increase in the SOD, on the other hand, resulted in an increase in surface roughness, VMRR, and a decrease in dimensional accuracy. Furthermore, increasing the abrasive flow rate resulted in lower surface roughness and dimensional accuracy while achieving a higher VMRR. The Institution of Engineers (India) 2023.
Kavya, J.T.; Keshavamurthy, R.; Ravishankar, M.K.; Pradeep Kumar, G.S.
Journal of The Institution of Engineers (India): Series D;Volume;106;Issue;1;pp.145-155
https://doi.org/10.1007/s40033-023-00597-1 https://www.scopus.com/pages/publications/85178937550?origin=resultslist
Kavya J.T., Department of Automobile Engineering, Dayananda Sagar College of Engineering, Bangalore, 560111, India; Keshavamurthy R., Department of Mechanical Engineering, Dayananda Sagar College of Engineering, Bangalore, 560111, India; Ravishankar M.K., Department of Automobile Engineering, Malnad College of Engineering, Hassan, 573202, India; Pradeep Kumar G.S., Department of Mechanical and Automobile Engineering, Christ University, Bengaluru, 560074, India
A Family of Mexican Hat Wavelet Stieltjes Transform for Unbounded Non-decreasing Functions
Mexican hat wavelet transform; Stieltjes and Lebesgue integral; Wavelet transform
In the present article, we examine the characteristics of the Mexican hat wavelet Stieltjes transform (MHWST) for a specific set of functions belonging to one of the sub-class of bounded variation functions. The subset comprises functions that are unbounded and non-decreasing. Further, a unified approach is applied to establish a uniqueness theorem and subsequently derive a representation theorem for the MHWST. The Author(s), under exclusive licence to The National Academy of Sciences, India 2024.
National Academy Science Letters;Volume;48;Issue;3;pp.379-383
https://doi.org/10.1007/s40009-024-01455-8 https://www.scopus.com/pages/publications/85205550656?origin=resultslist
Rawat A., Department of Sciences and Humanities, CHRIST (Deemed to be University), Bangalore, India; Singh A., Department of Mathematics and Statistics, Banasthali Vidhyapith, Banasthali, India
A Comparative Study of Nutrient Composition, Proteolytic Activity, Phytochemical Profiles, Vitamin C Content, and Antioxidant Properties in the Peels of Selected Perennial Fruits
Anti-nutritional constituents; Fruit peels; Nutritional analysis; Phytochemical analysis; Ripening stage
The escalating global demand for fruits has led to a surge in fruit production, resulting in significant fruit waste, particularly peels. The present study aims to investigate the nutrient content, proteolytic activity, phytochemical levels, vitamin C and antioxidant properties of five perennial fruits, namely Carica papaya (papaya), Selenicereus costaricensis (Red dragon fruit), Ananas comosus (Pineapple), Musa acuminata (Cavendish banana), Punica granatum (Pomegranate) peels of varying ripening stages. Accordingly, two ripening stages for pomegranate, papaya and dragon fruit (PoR1 and PoR2; PaR1 and PaR2; DR1 and DR2, respectively) and three stages for banana and pineapple (BR1, BR2 and BR3; PiR1, PiR2 and PiR3, respectively) were identified based on ethylene gas emission. The elemental analysis showed that fruit peels of Pineapple (PiR3), Banana (BR2), Papaya (PaR2), and Dragon fruit (DR2) showed significantly higher content of macro and micro-elements compared to the other ripening stages. Pomegranate peels exhibited the highest proteolytic activity (5.09 0.98unitsg?1), total phenolics (246.09 0.25mgg?1), total flavonoids (158.27 1.72mgg?1), tannins (103.94 0.09mgg?1), DPPH scavenging activity (129.43 1.34%), and antioxidant activity (127.14 1.35mgg?1 by phosphomolybdate assay). A. comosus peels had the greatest vitamin C levels (95.53 3.52mgg?1). Anti-nutrient analysis revealed safe levels of oxalates, phytates, and alkaloids, except for high oxalate levels in pomegranate peels. Notably, all parameters exhibited an increasing trend with ripening stages, with a decline during senescence in Banana (BR3) and Pomegranate peel (PoR2). This knowledge of fruit peel composition can enhance their utilization by humans, pharmaceutical and food industries, while also contributing to more effective waste management. Our study addresses the pressing need for sustainable fruit peel utilization in the context of escalating fruit production and waste. The Author(s), under exclusive licence to National Academy of Agricultural Sciences 2024.
Gopalraaj, Jhanani; Velayudhannair, Krishnakumar
Agricultural Research;Volume;14;Issue;4;pp.742-753
https://doi.org/10.1007/s40003-024-00798-4 https://www.scopus.com/pages/publications/85207252142?origin=resultslist
Gopalraaj J., Department of Life Sciences, CHRIST (Deemed to Be University), Bangalore Central Campus, Hosur Main Road, Dharmaram Post, Karnataka, Bengaluru, 560 029, India; Velayudhannair K., Department of Life Sciences, CHRIST (Deemed to Be University), Bangalore Central Campus, Hosur Main Road, Dharmaram Post, Karnataka, Bengaluru, 560 029, India
Influence of high-shear exfoliation and the stabilizer on the formation of exfoliated graphene nanosheets and its supercapacitive performances
Graphite flakes; High-shear exfoliation; Liquid-phase exfoliation; Sodium dodecyl sulfate (SDS)
Despite the extensive research on the preparation of graphene nanosheets, there is no suitable and optimized procedure for the large-scale production of 'defect-free' graphene monolayers. In addition, there is only a few works on the eco-friendly shear exfoliation of graphite using water as the solvent. However, no work has been reported on optimizing the critical parameters such as rotation speed and time duration (rpm) of shear homogenizer and concentration of stabilizer that determine the quality of graphene nanosheets. In this paper, an eco-friendly and scalable approach to prepare graphene nanosheets from natural graphite flakes using a high-speed shear homogenizer and sodium dodecyl sulfate (SDS) as an ionic stabilizer has been reported. As a result, the obtained efficiency of exfoliated graphene corresponding to the optimized condition is found to be 89%. Later, the exfoliated graphene is characterized by both physical characterization (i.e., Raman spectroscopy and FE-SEM) and electrochemical characterization. The electrochemical analysis reveals that the prepared graphene nanosheets exhibit a specific capacitance of 60.24Fg?1. This method is simple, inexpensive, environmentally friendly, and easy to scale up. Iranian Chemical Society 2025.
Murugadoss, Vignesh; Reddy, Sushma N.; Subramania, Ashok Kumar; Dhandapani, Preethi; Rajendra, Saradh Prasad; AlSalhi, Mohamad S.; Angaiah, Subramania
Journal of the Iranian Chemical Society;Volume;22;Issue;4;pp.733-742
Springer Science and Business Media Deutschland GmbH
https://doi.org/10.1007/s13738-025-03180-5 https://www.scopus.com/pages/publications/105003022626?origin=resultslist
Murugadoss V., Electro-Materials Research Laboratory, Centre for Nanoscience and Technology, Pondicherry University, Puducherry, 605014, India; Reddy S.N., Electro-Materials Research Laboratory, Centre for Nanoscience and Technology, Pondicherry University, Puducherry, 605014, India, Department of Chemistry, Christ University, Bengaluru, 560029, India; Subramania A.K., Electro-Materials Research Laboratory, Centre for Nanoscience and Technology, Pondicherry University, Puducherry, 605014, India, Department of Biomedical Engineering, Saveetha Engineering College (Autonomous), Chennai, 602105, India, School of Biological and Health Systems Engineering, Arizona State University, Tempe, 85281, AZ, United States; Dhandapani P., Electro-Materials Research Laboratory, Centre for Nanoscience and Technology, Pondicherry University, Puducherry, 605014, India; Rajendra S.P., Department of Physics and Astronomy, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia; AlSalhi M.S., Department of Physics and Astronomy, College of Science, King Saud University, Riyadh, 11451, Saudi Arabia; Angaiah S., Electro-Materials Research Laboratory, Centre for Nanoscience and Technology, Pondicherry University, Puducherry, 605014, India
LSTM-MGTO: a novel early breast cancer detection using long short term memory based modified gorilla troops optimization algorithm
Intersection over Union (IoU) metric; LSTM-MGTO (a technique combining long short-term memory and modified Gorilla troop optimization); Mammary neoplasm; U-Net (a neural network architecture); WDBC and BCCD (two datasets related to breast cancer diagnosis)
One of the most prevalent and severe tumors in women, breast cancer, remains a major global health issue despite a notable increase in incidence over the last ten years. It is the second leading cause of cancer-related death among women. Identifying breast cancer in its early stages has the potential to save lives; however, current screening techniques for the illness require several laboratory procedures involving medical experts. Automated solutions with rapid and reliable diagnostic capabilities are needed to minimize human error and expedite breast cancer diagnosis. The projected accuracy of cancer diagnosis remains far from matching the precision offered by existing approaches, even with the research on automated systems for the disease being studied. This work suggests a long short-term memory-based modified Gorilla troop optimization (LSTM-MGTO) method for breast cancer classification in order to address these issues. The Mastectomy Koibra Dataset (BCCD) and Wisconsin Diagnostic Mastectomy (WDBC) datasets were used to test the suggested methods. First, the proposed system employs contrast-limited adaptive histogram equalization (CLAHE) to enhance the quality of digital mammograms. Furthermore, employ a semantic deep learning (SDL) model to extract features. After the feature selection process, a recursive feature elimination technique was implemented to determine the crucial WDBC and BCCD characteristics that are relevant to breast cancer detection. Moreover, recommend a modified U-Net architecture for partitioning in both unmapped and guided contexts. The experimental findings indicate that the newly developed partitioning model surpasses existing advanced techniques, yielding superior results in both Dice and IoU score evaluations. On the WDBC and BCCD datasets, the suggested U-Net segmentation produces maximum Dice scores of 97.65% and 96.24%, respectively. Additionally, the model obtained the greatest IoU scores of 95.43% and 90.65% on the WDBC and BCCD datasets, respectively, according to the experimental findings. The Author(s), under exclusive licence to Springer-Verlag GmbH Austria, part of Springer Nature 2025.
Umamaheswari, D.; Kannan, M.; Mary, I. Priya Stella; Rozario, D. Juliet; Savitha, P. Margaret; Manimekala, B.
Network Modeling Analysis in Health Informatics and Bioinformatics;Volume;14;Issue;1;Article No.;17;
https://doi.org/10.1007/s13721-025-00509-1 https://www.scopus.com/pages/publications/105000443937?origin=resultslist
Umamaheswari D., Department of Computer Science, Christ University, Karnataka, Bangalore, India; Kannan M., Department of Computer Science, Christ University, Karnataka, Bangalore, India; Mary I.P.S., Department of Computer Science, Christ University, Karnataka, Bangalore, India; Rozario D.J., Department of Computer Science, Christ University, Karnataka, Bangalore, India; Savitha P.M., Department of Computer Science, Christ University, Karnataka, Bangalore, India; Manimekala B., Department of Computer Science, Christ University, Karnataka, Bangalore, India
Effects of light-emitting diode (LED) light sources on in vitro protocorm-like body (PLB) proliferation, plantlet regeneration, and ex vitro acclimatization in Cymbidium Snow Pearl
One of the key environmental elements that influences plant growth in vitro is light quality. Currently, a variety of horticultural plants are regenerated in vitro using light-emitting diode (LED) light sources to produce healthy, high-quality plants that can adapt well to ex vitro transplantation conditions. Investigating the impact of various spectrum light sources at various phases of in vitro regeneration is essential, though. The objective of this research was to examine how red (R), blue (B), white (W), red plus blue (RB, 1:1), red, green (G), and blue (RGB, 1:1:1) LEDs affect the growth of protocorm-like bodies (PLBs), shoot regeneration, and the rooting stages of shoots. The findings showed that B-LEDs were accountable for PLB proliferation, whereas R-LEDs were responsible for increased shoot regeneration and improved growth matrices with shoots and plantlets as compared to other LED treatments. Plant height, leaf count, and dry matter percentage were all higher in the plantlets that were regenerated under R-LED. On the other hand, more root regeneration and longer roots were caused by the B-LED treatment. Plants cultivated under RB LEDs had greater levels of carotenoid pigments, total chlorophyll, chlorophyll a, and chlorophyll b. When compared to other treatments, photosynthetic fluorescence characteristics like maximum quantum yield of PSII (Fv/Fm), photochemical quenching coefficient (qP), relative electron transport in PSII (ETRII), and non-photochemical quenching (NPQ) were lower in plants cultivated under R-LED treatment. The best LED for in vitro Cymbidium Snow Pearl plant regeneration was as follows: The B-LED was good for PLB proliferation, the R-LED was appropriate during shoot regeneration, and the growth of plantlets, the physiological characteristics such as chlorophyll a, chlorophyll b, total chlorophyll, and carotenoid content and number of epidermal cells per unit area were optimum with the plants grown under RB LED light. The Author(s), under exclusive licence to Korean Society for Horticultural Science 2026.
Kang, Beomsoo; Kim, Ye-Won; Lee, Han-Sol; Han, Jong-Eun; Murthy, Hosakatte Niranjana; Kim, Yae Jin; Park, Pil Man; Park, So-Young
Horticulture Environment and Biotechnology;
https://doi.org/10.1007/s13580-026-00819-x https://www.scopus.com/pages/publications/105040534912?origin=resultslist
Kang B., Department of Horticultural Science, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, South Korea; Kim Y.-W., Department of Horticultural Science, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, South Korea; Lee H.-S., Department of Horticultural Science, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, South Korea; Han J.-E., Department of Horticultural Science, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, South Korea, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 181 Ipsin-gil, Jeonbuk, 56212, South Korea; Murthy H.N., Department of Horticultural Science, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, South Korea, Department of Life Sciences, Christ University, Bengaluru, 560029, India; Kim Y.J., National Institute of Horticultural and Herbal Science, Rural Development Administration, 385 Seodun-ro, Gyeonggi-do, Suwon-si, South Korea; Park P.M., National Institute of Horticultural and Herbal Science, Rural Development Administration, 385 Seodun-ro, Gyeonggi-do, Suwon-si, South Korea; Park S.-Y., Department of Horticultural Science, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, South Korea
Effects of citric acid, ascorbic acid, and polyvinylpyrrolidone in overcoming medium browning during micropropagation of Phalaenopsis univivace
Adsorbent; Antioxidants; In vitro regeneration; Orchid; Tissue culture
The most significant issues in the early phases of plant tissue culture are the browning of the explant and media. The objective of this research was to determine how well citric acid (CTR, 20 and 40 mg L? 1), ascorbic acid (ASA, 200 and 400 mg L? 1), and polyvinylpyrrolidone (PVP, 20 and 40 mg L? 1) could help Phalaenopsis Univivace overcome medium browning during the stages of shoot multiplication (MS medium supplemented with 150 mL L? 1 coconut water, 10 mg L? 1 adenine sulfate, 1 mg L? 1 thidiazuron, and 15g L? 1 sucrose) and rooting of shoots (MS medium with 25 mL L? 1 coconut water, 1 mg L? 1 indole butyric acid, 15g L? 1 sucrose). Numerous metrics were estimated, including the number of shoots that were regenerated during the shoot regeneration stage, the number of roots that were regenerated during the shoot rooting stage, and a number of growth parameters. The quantity of carotenoid and chlorophyll pigments, PSII quantum yield (Fv/Fm), the effective PSII quantum yield (YII), and the non-photochemical quenching (NPQ) were also measured. Additionally, oxidative stress enzyme malonaldehyde (MDA) and preventive antioxidant enzymes including catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) were measured in regenerated shoots and plantlets. The results showed that the medium browning issues during the shoot and root regeneration stages were resolved by supplementing with CTR, ASA, and PVP. In terms of shoot regeneration, rooting of shoots, and enhancing shoot and plantlet growth metrics, 40 mg L? 1 CTR supplementation was determined to be superior overall. The Author(s), under exclusive licence to Korean Society for Horticultural Science 2025.
Kang, Beomsoo; Kim, Ye-Won; Murthy, Hosakatte Niranjana; Ho, Thanh-Tam; Yoon, Yeo-Joong; An, Hae-Ryun; Lee, Su-Young; Park, So-Young
Horticulture Environment and Biotechnology;Volume;66;Issue;5;pp.1175-1187
https://doi.org/10.1007/s13580-025-00746-3 https://www.scopus.com/pages/publications/105013802919?origin=resultslist
Kang B., Division of Animal, Horticultural and Food Sciences, Department of Horticultural Science, Chungbuk National University, Cheongju, 28644, South Korea; Kim Y.-W., Division of Animal, Horticultural and Food Sciences, Department of Horticultural Science, Chungbuk National University, Cheongju, 28644, South Korea; Murthy H.N., Division of Animal, Horticultural and Food Sciences, Department of Horticultural Science, Chungbuk National University, Cheongju, 28644, South Korea, Department of Life Sciences, CHRIST (Deemed to be University), Bengaluru, 560029, India; Ho T.-T., Institute for Global Health Innovations, Duy Tan University, Hoa Khanh Nam Campus 120 Hoang Minh Thao, Da Nang, 550000, Viet Nam; Yoon Y.-J., YooniPlant Tech Co., Umseong, Chungbuk, 27660, South Korea; An H.-R., Floriculture Research Division, National Institute of Horticultural & Herbal Science, Wanju, 55365, South Korea; Lee S.-Y., Floriculture Research Division, National Institute of Horticultural & Herbal Science, Wanju, 55365, South Korea; Park S.-Y., Division of Animal, Horticultural and Food Sciences, Department of Horticultural Science, Chungbuk National University, Cheongju, 28644, South Korea
Anti-inflammatory and anti-diabetic activities of lanthanum oxide nanoparticles using Plectranthus amboinicus leaf extract
Anti-diabetic; Anti-inflammatory; Green synthesis; Lanthanum oxide NPs; Morphology; Structural
Lanthanum oxide nanoparticle (La2O3 NPs) were synthesized by co-precipitation technique using Plectranthus amboinicus (P. amboinicus ) leaf extract. A yellowish green color was observed after the addition of leaf extract to the NaOH solution. The synthesis of nanoparticles plays a vital role in the field of science and technology. The cubic structure of the La2O3 was confirmed by Powder XRD. The functional groups present in the NPs were confirmed by FT-IR spectroscopy. The absorbance spectrum was observed at 274nm in the wavelength range of 230850nm. The calculated band gap value was 4.32eV. The structural morphology of La2O3 NPs was observed as cubic and irregular shape obtained from SEM image and EDAX spectrum which confirms the presence of La and O elements. The average particle sizes of the NPs were observed to be 40.22nm analyzed by high resolution transmission electron microscopy (HR-TEM) analysis. The main objective of this research work was focused on prepared La2O3 NPs act as a potential inhibitor to handle various inflammations and diabetes problems. Bovine serum albumin (BSA) denaturation approach produced a strong anti-inflammatory response with 92.89% inhibition at 500g/mL while ?-amylase showed significant antidiabetic activity of about 94.55% inhibition at 500g/mL. These results suggest that the green synthesized nanoparticles can be used for ?-amylase and BSA denaturation inhibitory activities, which may be crucial for biomedical applications. The Author(s), under exclusive licence to Society for Plant Biochemistry and Biotechnology 2026.
Vijayan, Kousalya Devi; Alagarsamy, Nivedhitha Bharathi; Sangaraju, Sambasivam; Arumugam, Venkatesan; Muthusamy, Krishna Kumar; Sakkarapani, Sudhahar
Journal of Plant Biochemistry and Biotechnology;
https://doi.org/10.1007/s13562-026-01032-8 https://www.scopus.com/pages/publications/105032119584?origin=resultslist
Vijayan K.D., Department of Physics, Alagappa University, Karaikudi, 630003, India; Alagarsamy N.B., Department of Physics, Alagappa University, Karaikudi, 630003, India; Sangaraju S., National Water and Energy Center, United Arab Emirates University, Al Ain, 15551, United Arab Emirates; Arumugam V., Department of Physics, Panimalar Engineering College, Tamilnadu, Chennai, 600123, India; Muthusamy K.K., Department of Physics and Electronics, CHRIST (Deemed to be University), Bengaluru, 560029, India; Sakkarapani S., Department of Physics, Alagappa University, Karaikudi, 630003, India
Effect of Chelating Agent Concentration on the Pseudocapacitive Performance of V2O5 Flakes Prepared by the Hydrothermal Process for Supercapacitor Applications
Cyclic voltammetry; Pseudo capacitance; SEM; V2O5 flakes; XRD
Vanadium pentoxide (V?O?) flakes were synthesized via a hydrothermal method by varying the amount of lemon juice (5mL for sample-1 and 10mL for sample-2) as a natural chelating agent. Structural and morphological analyses were performed using X-ray diffraction (XRD) and scanning electron microscopy (SEM), confirming crystalline V?O? with flake-like morphologies influenced by chelating agent concentration. Electrochemical performance was evaluated using cyclic voltammetry (CV), galvanostatic chargedischarge (GCD), and electrochemical impedance spectroscopy (EIS) in a 3M KOH electrolyte. Sample-2 exhibited a Significantly higher Specific capacitance of 1536 F?g?1 (CV at 1mVs?1) and 212.37 F?g?1 (GCD at 1 A?g?1) compared to sample-1, demonstrating that increasing lemon juice concentration enhances the capacitive behavior of V?O? flakes by improving ion diffusion and electroactive surface area. The Author(s) under exclusive licence to Sociedade Brasileira de Fica 2025.
Sattar, Sheik Abdul; Kumar, Kilari Naveen; Vinay, S.K.; Chandrakant, Kotian Akshata; Ashok Reddy, G.V.; Jafri, R Imran; Radhalayam, Dhanalakshmi; Prakash, Nunna Guru; Rosaiah, P.; Ali, Atif Mossad; Ko, Tae Jo
Brazilian Journal of Physics;Volume;55;Issue;6;Article No.;263;
https://doi.org/10.1007/s13538-025-01896-6 https://www.scopus.com/pages/publications/105016394453?origin=resultslist
Sattar S.A., Nitte Meenakshi Institute of Technology (NMIT), Nitte (Deemed to Be University), Karnataka, Bengaluru, 560064, India; Kumar K.N., Nitte Meenakshi Institute of Technology (NMIT), Nitte (Deemed to Be University), Karnataka, Bengaluru, 560064, India; Vinay S.K., Department of Physics, REVA University, Bangalore, 560064, India; Chandrakant K.A., Nitte Meenakshi Institute of Technology (NMIT), Nitte (Deemed to Be University), Karnataka, Bengaluru, 560064, India; Ashok Reddy G.V., Nitte Meenakshi Institute of Technology (NMIT), Nitte (Deemed to Be University), Karnataka, Bengaluru, 560064, India; Jafri R.I., Department of Physics and Electronics, Christ University, Hosur Road, Bengaluru, 560029, India; Radhalayam D., Department of Physics, University of Santiago of Chile (USACH), Santiago, 9170124, Chile; Prakash N.G., School of Mechanical Engineering, Yeungnam University, 280 Daehak-Ro, Gyoungsan-si, Gyeongsangbuk-do, Gyoungsan-si, 38541, South Korea; Rosaiah P., Department of Physics, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences (SIMATS), Thandalam, Chennai, 602 105, India; Ali A.M., Department of Physics, Faculty of Science, King Khalid University, Abha, Saudi Arabia; Ko T.J., School of Mechanical Engineering, Yeungnam University, 280 Daehak-Ro, Gyoungsan-si, Gyeongsangbuk-do, Gyoungsan-si, 38541, South Korea
Kinetic characterisation of proteases from Punica granatum, Musa acuminata, Carica papaya, and Ananas comosus as sustainable enzyme sources
Circular economy; Enzyme kinetics; agro-industrial waste utilisation; Sustainable bioprocessing
Proteases are vital industrial enzymes, contributing approximately 60% of the global enzyme market, by facilitating protein hydrolysis. Fruit peels, a major agricultural waste, offer a sustainable alternative for commercial enzyme production. This study investigates the proteases extracted from the peels of Punica granatum, Musa acuminata, Carica papaya, and Ananas comosus, with a primary focus on determining their optimal pH, temperature, and substrate specificity. Additionally, K? and V??? kinetics were assessed to characterize their catalytic efficiency. Optimal proteolytic activity was observed at pH 8 and 30C for P. granatum, pH 7 and 30C for M. acuminata, pH 8 and 30C for C. papaya, and pH 7 and 50C for A. comosus. substrate specificity of protease was assessed using casein, fish meal, soybean meal, black soldier fly larvae, bovine serum albumin, and egg albumin, revealing broad applicability, especially in P. granatum peels. The stability of P. granatum proteases across substrates suggests multiple isoforms or a flexible active site. Kinetic analysis using Lineweaver-Burk plots revealed Vmax and KM values of 8.45 mol/min/mL and 3.81 M (P. granatum), 4.56 mol/min/mL and 10.08 M (M. acuminata), 2.98 mol/min/mL and 2.84 M (C. papaya), and 2.97 mol/min/mL and 11.38 M (A. comosus) respectively. Among the tested fruit peels, P. granatum exhibited the highest reaction rate, while C. papaya demonstrated the highest substrate affinity, making them as promising candidates for feed supplementation and industrial enzyme applications. The broad substrate specificity and high catalytic efficiency of P. granatum further reinforce its potential for use in feed formulations, enhancing protein hydrolysis and improving nutrient availability. These findings highlight the significant potential of fruit peel-derived proteases in promoting sustainable enzyme production and advancing bioeconomic applications. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2026.
Biomass Conversion and Biorefinery;Volume;16;Issue;1;Article No.;14;
https://doi.org/10.1007/s13399-025-07037-y https://www.scopus.com/pages/publications/105026360855?origin=resultslist
Gopalraaj J., Department of Life Sciences, Christ University, Hosur Main Road, Dharmaram College Post, Karnataka, Bengaluru, 560 029, India; Velayudhannair K., Department of Life Sciences, Christ University, Hosur Main Road, Dharmaram College Post, Karnataka, Bengaluru, 560 029, India
Valorization of waste chilli stalks (Capsicum annuum) as a sustainable substrate for cellulose extraction: insights into its thermomechanical, film forming and biodegradation properties
Biodegradation; Biopolymer; Cellulose; Cellulose sheet; Chilli waste; Valorization
Rising global population accelerates food waste generation, thereby creating a crisis in food waste management. A solution involves deriving value-added products like cellulose biopolymer from food waste. Chilli stalk wastes are one such food waste which are generated in large quantities and are unsuitable for field use or incineration due to health and environmental challenges. A greener alternative is extracting cellulose biopolymer from chilli stalk waste. The extraction of cellulose biopolymer from chilli stalk results in a renewable, biodegradable and economically efficient biomaterial with a broad range of applications. The extraction process involving alkali treatment (NaOH) and bleaching (alkaline H2O2), resulted in a yield of 29.85% cellulose biopolymer. The extracted cellulose was subjected to quantification and functional property analysis followed by characterization (FTIR, XRD, TGA, DSC and SEM) to analyse functional groups, crystallinity, thermal properties and surface morphology. Functional property analysis resulted in higher values when compared with commercial cellulose. The characterization techniques confirmed the effective removal of impurities such as lignin, hemicellulose and pectin by the chemical treatments. Cellulose sheets, fabricated using solvent casting, exhibited exceptional biodegradability (85.36%) within 20days, surpassing conventional food packaging materials, commercial food packaging paper (15.95 0.12% [%w/w]) and plastic sheets (7.89 0.33% [%w/w]) over the same time period. The novelty of this research lies in the innovative valorization of chilli stalk waste, which often remains unused in large quantities globally. This study introduces a cost-effective method to convert it into a value-added, highly biodegradable biopolymer. The resulting cellulose sheets provide an eco-friendly substitute for traditional food packaging materials. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024.
Santhosh, Adhithya Sankar; Umesh, Mridul
Biomass Conversion and Biorefinery;Volume;15;Issue;21;pp.28195-28208
https://doi.org/10.1007/s13399-024-05370-2 https://www.scopus.com/pages/publications/85183698575?origin=resultslist
Santhosh A.S., Department of Life Sciences, CHRIST (Deemed to be University), Hosur Road, Karnataka, Bengaluru, 560029, India; Umesh M., Department of Life Sciences, CHRIST (Deemed to be University), Hosur Road, Karnataka, Bengaluru, 560029, India
Eco-conscious photocatalytic degradation of organic textile dyes using green synthesized silver nanoparticles: a safe and green approach toward sustainability
Green synthesis; Organic dyes; Photocatalyst; Phytotoxicity; Strobilanthes barbatus leaf extracts
Green synthesized nanoparticles from Strobilanthes barbatus leaf extracts are environmentally safe and feasible for enduring wastewater treatment, especially for organic textile dye degradation. The synthesized Strobilanthes barbatusmediated silver/silver-oxide nanoparticles (SB-Ag/AgO NPs) showed maximum absorbance at 428nm. The SB-Ag/AgO NPs were generally spherical with an average diameter of 37.59nm (FESEM and TEM analysis). The importance of functional groups in the production of SB-Ag/AgO NPs was recorded by FTIR investigations. In the degradation and rate of degradation for textile dyes, after 320min, SB-Ag/AgO NPs displayed 96.60% (5.31 10?1 L mg?1min?1) and 87.50% (1.179 10?1 L mg?1min?1) degradation of Reactive Blue 220 (RB-220) and Reactive Blue 222A (RB-222A), respectively. When compared to dye effluents, SB-Ag/AgO NPs-treated dye solutions revealed a considerable decrease in inhibitory efficiency during phytotoxicity evaluation on test organisms, Vigna radiata and Artemia salina. The biosynthesized SB-Ag/AgO NPs could serve as a feasible photocatalyst for the treatment of organic textile dyes in organic substancepolluted water ecosystems. SB-Ag/AgO NPs can serve as efficient, cost-effective and environmentally friendly sources for dye degradation. The current research offers a safe and environmentally friendly strategy for sustaining the environment. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2024.
Gangwar, Jaya; Joseph, Kadanthottu Sebastian
Biomass Conversion and Biorefinery;Volume;15;Issue;17;pp.23989-24000
https://doi.org/10.1007/s13399-024-05348-0 https://www.scopus.com/pages/publications/85183702608?origin=resultslist
Gangwar J., Department of Life Sciences, Christ University, Karnataka, Bangalore, 560029, India; Joseph K.S., Department of Life Sciences, Christ University, Karnataka, Bangalore, 560029, India
Valorization of bovine tannery fleshing waste as a fish meal replacer: a conceivable green approach
Bovine tannery fleshing waste; Fermentation; Fish meal replacer; Resource recovery; Solid waste management
The study intended to utilize bovine tannery fleshing (BTF) a significant contributor of solid wastes in leather industries, in its unhydrolyzed form (raw fleshing flour [RFF]) as well as in fermented form (fermented fleshing flour [FFF]) as fish meal (FM) replacer in formulated aqua feeds. In line with this, experimental diets were formulated, characterized, and evaluated for their virtue. Improvement in the physical quality of diets was achieved with FFF incorporation, enabling better pelletability, palatability, and feed texture. As the formulated diets were of sinking type, feeding trials on column as well as bottom feeders were concertedly attempted, as a value-added application of proteinaceous feed ingredient (BTF) in aquaculture sector. FFF inclusion diets proved superior to RFF inclusion diets. Maximum protein and lipid retentions of 89% and 92% were evidenced. Fish feeding experiments disclosed the productive impact of substituting FM with FFF by 50 and 75% on the performance (fish growth) of Cirrhinus mrigala (19.03 cm final length, 112.89 g Kg-1 final body weight, and 99% survival rate) and Labeo rohita (22.19 cm final length, 214.99 g Kg-1 final body weight, and 97% survival rate) respectively, with enhanced muscle biochemical compositions. FFF inclusion diets best suited column feeders than bottom feeders, with acceptable feed conversion ratio (< 2) and about 65% and 69% of protein in Rohu and Mrigal, respectively after 90 days of feeding. Absence of feed related mortality and multimycotoxins in FFF inclusion diets authenticated its invulnerable nature, signifying the need of bacterial fermentation processes to pull out the maximum worth of the solid waste. Thus, bovine tannery fleshing (BTF) would probably be used as a relatively inexpensive, effectual, safe, and an absolute source of protein for marine animals, reinforcing aquaculture business to strengthen up its profitability and eventually; this is an assuring conscientious solid waste management strategy that could be scaled-up to develop high-quality aquaculture feeds. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023.
Biomass Conversion and Biorefinery;Volume;15;Issue;15;pp.22437-22457
https://doi.org/10.1007/s13399-023-05033-8 https://www.scopus.com/pages/publications/85175620400?origin=resultslist
Thazeem B., Waste Management Division, Integrated Rural Technology Centre (IRTC), Kerala, Palakkad, 678592, India; Umesh M., Department of Life Sciences, CHRIST (Deemed to be University), Hosur Road, Karnataka, Bengaluru, 560029, India
Floral waste as a potential feedstock for polyhydroxyalkanoate production using halotolerant Bacillus cereus TS1: optimization and characterization studies
Bacillus sp; Biopolymers; Characterization; Floral waste; Optimization; Polyhydroxyalkanoates
The versatile properties and high degree of biodegradability of polyhydroxyalkanoates (PHA) have made them the ideal candidate for biomedical and other applications. Although extensive research on PHA-producing bacterial isolates from terrestrial environments is documented in the available literature, the potential of marine bacterial isolates in PHA production remains less explored and offers a great scope for future research. This research work primarily focuses on isolation and characterization of PHA-producing bacterial isolates from samples collected from coastal areas of Kerala, India. Furthermore, the possibility of PHA production from the most potential isolate Bacillus cereus TS1 using jasmine waste hydrolysate-based media was explored in this study. The utilization of floral waste hydrolysate (FWH) for PHA fermentation is not widely discussed in the available literature and is the major novelty factor of this research work. Under optimized conditions of glucose (1.2% w/v), yeast extract (0.15% w/v), NaCl (5.02% w/v), and incubation period (60h), a maximum PHA yield of 1.13g/L was achieved. The characterization of PHA polymer was done using Fourier transform infrared (FTIR) spectroscopy, nuclear magnetic resonance (NMR), X-ray diffraction (XRD) and thermogravimetric analysis (TGA). Thus, this research work integrates floral waste valorisation with microbial biopolymer production and highlights an innovative approach for sustainable development. The scale of this method on an industrial scale in future may prove helpful in the cost-effective production of PHA using cheap raw materials. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023.
Biomass Conversion and Biorefinery;Volume;15;Issue;15;pp.22517-22528
https://doi.org/10.1007/s13399-023-05003-0 https://www.scopus.com/pages/publications/85174599251?origin=resultslist
Grigary S., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bangalore, India; Umesh M., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bangalore, India
Novel biocompatible zinc oxide nanoparticle synthesis using Quassia indica leaf extract and evaluation of its photocatalytic, antimicrobial, and cytotoxic potentials
Antimicrobial; Cytotoxic; Degradation; Green synthesis; Quassia indica; Textile dyes; Zinc oxide nanoparticles
Prognostic research points to the necessity and relevance of revamping polluted environments. The toxic effect of textile dyes released into waterbodies can be reduced by the degradation process and alternate methods in nanotechnology are used to lessen the gravity of the situation. Compared with chemical and physical NP synthesis, plant extract-based nanoparticle synthesis is an environmentally friendly alternative method, and the use of waste leaves in this process is an added advantage. Quassia indica zinc oxide nanoparticles (QI-ZnO NPs) were synthesised in the current work employing a simple and cost-effective process using Q. indica leaf extract. The surface plasmon peak was visible in the UV-Vis absorption spectrum of the decreased reaction mixture at 346 nm. The average crystallite size of the QI-ZnO NPs was found to be 16.66 nm. The QI-ZnO NPs were found to have a stable zeta potential of ?28.4 mV. The surface morphology of the optimised QI-ZnO NPs was observed to be hexagonal using field emission scanning electron microscopy and high-resolution transmission electron microscopy. Under UV light irradiation, the photocatalytic degradation of industrial textile dyes Reactive Blue-220, Reactive Yellow-145, Reactive Red-120, and Reactive Blue-222 showed degradation efficiency of 8090%. Antibacterial and antifungal activity was assessed using well diffusion on gram-positive and gram-negative microorganisms. When administered to the A549 and MDA-MB-231 cancer cell lines, QI-ZnO NPs displayed significant anticancer activities. Limited studies in the area of plant extract-based nanoparticle synthesis mark the novelty of this attempt and this trailblazing and pioneering approach using non-toxic QI-ZnO NPs synthesised through green synthesis is futuristic and sustainable helping in effective wastewater treatment. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023.
Scaria, Shilpa Susan; Sebastian, Joseph Kadanthottu
Biomass Conversion and Biorefinery;Volume;15;Issue;19;pp.26545-26564
https://doi.org/10.1007/s13399-023-04989-x https://www.scopus.com/pages/publications/85174828786?origin=resultslist
Scaria S.S., Department of Life Sciences, Christ University, Karnataka, Bangalore, 560029, India; Sebastian J.K., Department of Life Sciences, Christ University, Karnataka, Bangalore, 560029, India
Bioconversion of chicken feather waste into feather hydrolysate by multifaceted keratinolytic Bacillus tropicus LS27 and new insights into its antioxidant and plant growth-promoting properties
Antioxidants; Chicken feather; Feather hydrolysate; Heavy metal; Keratin; Metal tolerance; Plant growth
Abstract: Keratin, the main structural constituent of feathers, contains a lot of valuable amino acids which are potential bioactive compounds as well. Since conventional methods are not efficient enough to achieve complete removal of chicken feather waste, biological mode of feather degradation is one of the most appropriate ways to utilize feathers, thereby reducing wastes as well as generating value-added products from feathers. This study was focussed on valorizing chicken feather into feather hydrolysate (FH) containing bioactive compounds for plant growth promotion. Keratinolytic bacteria capable of degrading chicken feathers were isolated from the poultry waste dumping site of Russell Market, Shivajinagar, Bangalore, Karnataka, India. The isolated bacteria was identified as Bacillus tropicus LS 27. A minimal media with chicken feather as the sole source of carbon and nitrogen was prepared and inoculated with Bacillus tropicus LS 27 [5% (v/v)]. Degradation of keratin protein by bacteria caused the solubilization of amino acids which was confirmed by high-performance liquid chromatography (HPLC) analysis where an appreciable amount of amino acids like cysteine, valine, isoleucine, proline, lysine, methionine, and phenylalanine was detected. The Fourier transform infrared spectroscopy (FTIR) analysis of hydrolysed chicken feathers showed C=0 stretching, S-H bond stretching, and formation of carboxylic acid groups indicating effective degradation of chicken feathers. Scanning electron microscope (SEM) images revealed the degradation pattern of feathers showing complete degradation of barbs and barbules with a portion of rachis remaining. Feather hydrolysate was further explored for its antioxidant activity using DPPH scavenging assay, and the value was found to be 1.5 mg/mL. The bacterial cells when screened for heavy metal tolerance showed significant metal tolerance to lead (Pb) and chromium (Cr). Since Bacillus tropicus LS27 showed indole-3-acetic acid (IAA), siderophore, and ammonia production, the prepared feather hydrolysate along with the bacterial cells were used as soil amendment for plant growth studies over Spinacia oleracea L. The study revealed that plants supplemented with 20% (v/v) FH showed elevated plant growth, therefore proving to be optimum for the support of plant growth. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023.
Biomass Conversion and Biorefinery;Volume;15;Issue;21;pp.28063-28073
https://doi.org/10.1007/s13399-023-04664-1 https://www.scopus.com/pages/publications/85165582629?origin=resultslist
Liya S.M., Department of Life Sciences, CHRIST (Deemed to be University), Hosur Road, Karnataka, Bengaluru, 560029, India; Umesh M., Department of Life Sciences, CHRIST (Deemed to be University), Hosur Road, Karnataka, Bengaluru, 560029, India
Fatigue surface analysis of AL A356 alloy reinforced hematite metal matrix composites
Al A356; Hematite; Fatigue strength; Fractography; Microstructure
This study intends to investigate how copper chill affects the fatigue behaviour of composites made of aluminium alloy A356 and hematite. It was cast by altering the weight fraction particles of hematite (0 to 12%wt in increments of 3%wt) by sand casting method with and without copper chills at its end to get isotropic and homogenous significant characteristics under liquid metallurgical way. The test specimens were prepared in accordance with ASTM specifications. Ducom-type fatigue testing equipment (rotating bending-low cycle fatigue) is used in experiments to examine fatigue behaviour. The micrographic images were taken with a scanning electron microscope (SEM) and interpreted uniform reinforcement of hematite particles, and X-ray diffraction (XRD) patterns were used to reveal microscopic details. The existence of the hematite particles and their phases was revealed by the X-ray diffraction analysis. The results show that the composites cast with copper chills have significantly greater fatigue strength than the casting obtained without copper chills. It was also observed that at 9%wt, copper chilled composite shows improve in fatigue strength about 10.2% as compared without chilled composites. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023.
Kumar, M. Sunil; Sathisha, N.; Manjnatha, S.; Niranjana, S.J.; Tamam, Nissren; Khan, M. Ijaz
Biomass Conversion and Biorefinery;Volume;15;Issue;15;pp.21911-21923
https://doi.org/10.1007/s13399-023-04634-7 https://www.scopus.com/pages/publications/85166355390?origin=resultslist
Kumar M.S., VTU-RRC, Karnataka, Belagavi, India; Sathisha N., Department of Mechanical Engineering, Yenepoya Institute of Technology, Karnataka, Mangalore, India; Manjnatha S., Department of Mathematics, School of Engineering and Technology, CHRIST (Deemed to Be University), Karnataka, Bengaluru, 560076, India; Niranjana S.J., Department of Mechanical Engineering, School of Engineering and Technology, CHRIST (Deemed to Be University), Karnataka, Bengaluru, 560076, India; Tamam N., Department of Physics, College of Science, Princess Nourah Bint Abdulrahman University, P.O. Box 84428, Riyadh, 11671, Saudi Arabia; Khan M.I., Department of Mathematics and Statistics, Riphah International University I-14, Islamabad, 44000, Pakistan, Department of Mechanical Engineering, Lebanese American University, Kraytem, Beirut, Lebanon
A sustainable approach for fish waste valorization through polyhydroxyalkanoate production by Bacillus megaterium NCDC0679 and its optimization studies
Bacillus megaterium; BoxBehnken design; Fish scale hydrolysate; PHA; PlackettBurman design; Response surface methodology
Polyhydroxyalkanoates (PHAs) are considered as the only class of truly biodegradable and biocompatible polymers. Although extensive research has been carried out in producing them from a wide variety of organisms, their commercialization still faces hurdles majorly associated with the cost of production media. This research work exploits the use of discarded fish scale waste as a major media component for biopolymer production. The major novelty of the research work is the utilization of a Bacillus megaterium NCDC0679 for PHA production using fish scale waste that is not reported previously. Furthermore, a sequential and systematic statistical optimization strategy employing response surface methodology was used to trace out the level of the most significant variables and their interaction effects on PHA production add to the significant novelty of this work. The significance of the model developed was determined from the p values of ANOVA. Under optimized levels of glucose (50g/L), NaCl (0.125g/L), and fish scale hydrolysate concentration (62.5% v/v), maximum PHA yield of 6.33g/L was achieved in the shake flask culture system. This was found to be 5.50-fold higher than the unoptimized medium. The ANOVA results established the significance of the model (p < 0.05). The extracted polymer was characterized through Fourier-transform infrared (FTIR), nuclear magnetic resonance (NMR), X-ray diffraction (XRD), differential scanning calorimetry (DSC), and thermogravimetric analysis (TGA). Thus, the present investigation suggests an innovative method for valorization of fish scale waste for commercial production of PHA. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022.
Umesh, Mridul; Suresh, Sreehari; Sarojini, Suma; Santosh, Adhithya Sankar
Biomass Conversion and Biorefinery;Volume;15;Issue;17;pp.23703-23715
https://doi.org/10.1007/s13399-022-03360-w https://www.scopus.com/pages/publications/85140085031?origin=resultslist
Umesh M., Department of Life Sciences, CHRIST (Deemed to be University), Hosur Road, Karnataka, Bengaluru, 560029, India; Suresh S., Department of Life Sciences, CHRIST (Deemed to be University), Hosur Road, Karnataka, Bengaluru, 560029, India; Sarojini S., Department of Life Sciences, CHRIST (Deemed to be University), Hosur Road, Karnataka, Bengaluru, 560029, India; Santosh A.S., Department of Life Sciences, CHRIST (Deemed to be University), Hosur Road, Karnataka, Bengaluru, 560029, India
Distillery effluent valorization through cost effective production of polyhydroxyalkanoate: optimization and characterization
Bacillus subtilis NCDC 0671; Distillery effluent; FTIR; Polyhydroxyalkanoate; Response surface methodology
The devastating effect of fossil plastics in the biosphere has tuned the concern for bioplastic production in the last few decades. Polyhydroxyalkanoate, a biopolyester, has a wide range of applications as they impose positive societal impact by being biodegradable and void of any ill-effects when used in vivo. Despite their eco-friendly nature, the outreach of PHA is bounded in industrial scale as the overall expense is highly comparable to conventional plastics. Therefore, in an attempt to attain a feasible production, the present study aims at utilizing raw distillery effluent for PHA production using Bacillus subtilis NCDC 0671. Different dilutions of spent wash (5%, 10%, 15%, and 20%) were assessed for PHA production in the modified medium among which 10% showed maximum PHA accumulation. Furthermore, statistical optimization by response surface methodology enhanced PHA synthesis to 6.3g/L which is 3.3-fold increases. FTIR and NMR characterization of the biopolymer from the optimized medium was similar to the previous literature which provides a promising approach for cost effective production. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2022.
Priyanka, Kumaresan; Umesh, Mridul; Preethi, Kathirvel
Biomass Conversion and Biorefinery;Volume;15;Issue;17;pp.23689-23701
https://doi.org/10.1007/s13399-022-03333-z https://www.scopus.com/pages/publications/85139403701?origin=resultslist
Priyanka K., Department of Microbial Biotechnology, Bharathiar University, Tamil Nadu, Coimbatore, 641046, India; Umesh M., Department of Life Sciences, CHRIST (Deemed to Be University), Hosur Road, Karnataka, Bengaluru, 560029, India; Preethi K., Department of Microbial Biotechnology, Bharathiar University, Tamil Nadu, Coimbatore, 641046, India