Assessing anticancer properties of PEGylated platinum nanoparticles on human breast cancer cell lines using in-vitro assays
- Title
- Assessing anticancer properties of PEGylated platinum nanoparticles on human breast cancer cell lines using in-vitro assays
- Creator
- Aswathy F.A.W.; Jose J.; Anila A.E.I.
- Description
- This study describes the in-vitro cytotoxic effects of PEG-400 (Polyethylene glycol-400)-capped platinum nanoparticles (PEGylated Pt NPs) on both normal and cancer cell lines. Structural characterization was carried out using x-ray diffraction and Raman spectroscopy with an average crystallite size 5.7 nm, and morphological assessment using Scanning electron microscopy (SEM) revealed the presence of spherical platinum nanoparticles. The results of energy-dispersive x-ray spectroscopy (EDX) showed a higher percentage fraction of platinum content by weight, along with carbon and oxygen, which are expected from the capping agent, confirming the purity of the platinum sample. The dynamic light scattering experiment revealed an average hydrodynamic diameter of 353.6 nm for the PEGylated Pt NPs. The cytotoxicity profile of PEGylated Pt NPs was assessed on a normal cell line (L929) and a breast cancer cell line (MCF-7) using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. The results revealed an IC50 of 79.18 ?g ml?1 on the cancer cell line and non-toxic behaviour on the normal cell line. In the dual staining apoptosis assay, it was observed that the mortality of cells cultured in conjunction with platinum nanoparticles intensified and the proliferative activity of MCF-7 cells gradually diminished over time in correlation with the increasing concentration of the PEGylated Pt NPs sample. The in vitro DCFH-DA assay for oxidative stress assessment in nanoparticle-treated cells unveiled the mechanistic background of the anticancer activity of PEGylated platinum nanoparticles as ROS-assisted mitochondrial dysfunction. 2024 IOP Publishing Ltd. All rights, including for text and data mining, AI training, and similar technologies, are reserved.
- Source
- Biomedical Physics and Engineering Express, Vol-10, No. 6
- Date
- 2024-01-01
- Publisher
- Institute of Physics
- Subject
- anticancer property; AO/PI dual staining; DCFH DA ROS assay; MTT assay; platinum nanoparticles; polyethylene glycol
- Coverage
- Aswathy F.A.W., Department of Physics and Electronics, Christ University, Karnataka, Bengaluru, 560029, India; Jose J., Division of Microbiology, Department of Biosciences, Rajagiri College of Social Sciences (Autonomous), Kerala, Cochin, 683104, India; Anila A.E.I., Department of Physics and Electronics, Christ University, Karnataka, Bengaluru, 560029, India
- Rights
- Restricted Access
- Relation
- ISSN: 20571976; PubMed ID: 39260382
- Format
- Online
- Language
- English
- Type
- Article
Collection
Citation
Aswathy F.A.W.; Jose J.; Anila A.E.I., “Assessing anticancer properties of PEGylated platinum nanoparticles on human breast cancer cell lines using in-vitro assays,” CHRIST (Deemed To Be University) Institutional Repository, accessed February 24, 2025, https://archives.christuniversity.in/items/show/12719.