Biological extraction of chitin from fish scale waste using proteolytic bacteria Stenotrophomonas koreensis and its possible application as an active packaging material
- Title
- Biological extraction of chitin from fish scale waste using proteolytic bacteria Stenotrophomonas koreensis and its possible application as an active packaging material
- Creator
- Suresh S.; Umesh M.; Santosh A.S.
- Description
- Chitin being the second most abundant polymer found in nature has extensive application and versatile material properties including biocompatibility. Extraction of chitin from diverse sources are majorly done using chemical extraction methods using high concentration of alkali that makes the method non eco-friendly and economically non-viable. This calls for eco-friendly methods of chitin extraction from cost-effective substrates through green methods. This research work presents a simplified one-step biological extraction of chitin from fish scales by successive fermentation using Stenotrophomonas koreensis isolated from soil. The fermentative approach for chitin extraction from fish scales using S. koreensis enzyme activity is not reported elsewhere in the available literature to the best of our knowledge. Chitin yield of 28% (w/w) was obtained after the successive fermentation. The extracted polymer was characterized using differential scanning calorimetry (DSC), Fourier transform infrared (FTIR), X-ray diffraction (XRD), and thermo gravimetric analysis (TGA). Furthermore, the possibility of converting extracted chitin into an active packaging material was explored by chemically, converting it to chitosan followed by analysis of its DPPH scavenging activity. The DPPH radical scavenging activity varied from 67.025 to 80.2%, which corresponds to 0.25 to 2mg/mL of chitosan. The chitosan films fabricated were subjected to biodegradation studies using soil burial method. Biodegradation rate of chitosan films was observed to be 21.49 0.62% (w/w) after 50days of incubation. Thus, the present research work highlights an integrated waste valorization strategy through microbial fermentation for commercially important biopolymer production. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature 2023.
- Source
- Biomass Conversion and Biorefinery, Vol-14, No. 22, pp. 29023-29033.
- Date
- 2024-01-01
- Publisher
- Springer Science and Business Media Deutschland GmbH
- Subject
- Antioxidant activity; Biodegradation; Chitin; Chitosan; Stenotrophomonas koreensis
- Coverage
- Suresh S., Department of Life Sciences, CHRIST (Deemed to Be University), Hosur Road, Karnataka, Bengaluru, 560029, India; Umesh M., Department of Life Sciences, CHRIST (Deemed to Be University), Hosur Road, Karnataka, Bengaluru, 560029, India; Santosh A.S., Department of Life Sciences, CHRIST (Deemed to Be University), Hosur Road, Karnataka, Bengaluru, 560029, India
- Rights
- Restricted Access
- Relation
- ISSN: 21906815
- Format
- Online
- Language
- English
- Type
- Article
Collection
Citation
Suresh S.; Umesh M.; Santosh A.S., “Biological extraction of chitin from fish scale waste using proteolytic bacteria Stenotrophomonas koreensis and its possible application as an active packaging material,” CHRIST (Deemed To Be University) Institutional Repository, accessed February 25, 2025, https://archives.christuniversity.in/items/show/12841.