A versatile sensor capable of ratiometric fluorescence detection of trace water and turn-on detection of Cu2+ modulating the binding interaction of a Cu(ii) complex with BSA and DNA complemented by docking studies
- Title
- A versatile sensor capable of ratiometric fluorescence detection of trace water and turn-on detection of Cu2+ modulating the binding interaction of a Cu(ii) complex with BSA and DNA complemented by docking studies
- Creator
- Vishnu S.; Nag A.; Das A.K.
- Description
- A fluorescent molecule, pyridine-coupled bis-anthracene (PBA), has been developed for the selective fluorescence turn-on detection of Cu2+. Interestingly, the ligand PBA also exhibited a red-shifted ratiometric fluorescence response in the presence of water. Thus, a ratiometric water sensor has been utilized as a selective fluorescence turn-on sensor for Cu2+, achieving a 10-fold enhancement in the fluorescence and quantum yield at 446 nm, with a lower detection limit of 0.358 ?M and a binding constant of 1.3 106 M?1. For practical applications, sensor PBA can be used to detect Cu2+ in various types of soils like clay soil, field soil and sand. The interaction of the PBA-Cu(ii) complex with transport proteins like bovine serum albumin (BSA) and ct-DNA has been investigated through fluorescence titration experiments. Additionally, the structural optimization of PBA and the PBA-Cu(ii) complex has been demonstrated by DFT, and the interaction of the PBA-Cu(ii) complex with BSA and ct-DNA has been analyzed using theoretical docking studies. 2024 The Royal Society of Chemistry.
- Source
- Analytical Methods, Vol-16, No. 30, pp. 5263-5271.
- Date
- 2024-01-01
- Publisher
- Royal Society of Chemistry
- Coverage
- Vishnu S., Department of Chemistry, Christ University, Hosur Road, Karnataka, Bangalore, 560029, India; Nag A., Department of Life Science, Christ University, Hosur Road, Karnataka, Bangalore, 560029, India; Das A.K., Department of Chemistry, Christ University, Hosur Road, Karnataka, Bangalore, 560029, India
- Rights
- Restricted Access
- Relation
- ISSN: 17599660; PubMed ID: 39015041
- Format
- Online
- Language
- English
- Type
- Article
Collection
Citation
Vishnu S.; Nag A.; Das A.K., “A versatile sensor capable of ratiometric fluorescence detection of trace water and turn-on detection of Cu2+ modulating the binding interaction of a Cu(ii) complex with BSA and DNA complemented by docking studies,” CHRIST (Deemed To Be University) Institutional Repository, accessed February 24, 2025, https://archives.christuniversity.in/items/show/12978.