Pemetrexed loaded gold nanoparticles as cytotoxic and apoptosis inducers in lung cancer cells through ROS generation and mitochondrial dysfunction pathway
- Title
- Pemetrexed loaded gold nanoparticles as cytotoxic and apoptosis inducers in lung cancer cells through ROS generation and mitochondrial dysfunction pathway
- Creator
- Baby Shakila P.; Periasamy T.; Alarfaj A.A.; Arulselvan P.; Ravindran R.; Suriyaprakash J.; Thangavelu I.
- Description
- Supramolecular nanoparticles containing peptides and drugs have recently gained recognition as an effective tumor treatment drug delivery system. A multitarget drug termed pemetrexed is effective against various cancers, including nonsmall cell lung cancer. The work aims to establish the capability of pemetrexed gold nanoparticles (PEM-AuNPs) to induce apoptosis and explore molecular changes. X-ray diffraction, Fourier-transform infrared spectroscopy, ultravioletvisible spectroscopy, scanning electron microscope, and transmission electron microscope were used to investigate the synthesized nanoparticles. The MTT assay was utilized to investigate the anticancer properties of PEM-AuNPs at varying concentrations (50, 100, and 200M). PEM-AuNPs demonstrated a decrease in cell viability with 55.87%, 43.04%, and 25.59% for A549 cells and 54.31%, 37.40%, and 25.84% for H1299 cells at the respective concentrations. To assess apoptosis and perform morphological analysis, diverse biochemical staining techniques, including acridine orange-ethidium bromide and 4?,6-diamidino-2-phenylindole nuclear staining assays, were employed. Additionally, 2?,7?-dichlorofluorescein diacetate staining confirmed the induction of reactive oxygen species generation, while JC-1 staining validated the impact on the mitochondrial membrane at the IC50 concentration of PEM-AuNPs. Thus, the study demonstrated that the synthesized PEM-AuNPs exhibited enhanced anticancer activity against both A549 and H1299 cells. 2024 International Union of Biochemistry and Molecular Biology, Inc.
- Source
- Biotechnology and Applied Biochemistry, Vol-71, No. 4, pp. 779-790.
- Date
- 2024-01-01
- Publisher
- John Wiley and Sons Inc
- Subject
- apoptosis; gold nanoparticles; lung cancer; mitochondrial damage; Pemetrexed; ROS generation
- Coverage
- Baby Shakila P., Department of Biochemistry, Vivekanandha College of Arts and Sciences for Women (Autonomous), Tiruchengode, Tamilnadu, Namakkal, India; Periasamy T., Department of Biochemistry, Muthayammal College of Arts and Science (Autonomous), Namakkal, India; Alarfaj A.A., Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia; Arulselvan P., Department of Chemistry, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, India; Ravindran R., Preclinical Department, Faculty of Medicine, Universiti Kuala Lumpur, Royal College of Medicine Perak, Ipoh, Malaysia; Suriyaprakash J., Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, School of Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou, China; Thangavelu I., Department of Chemistry, CHRIST (Deemed to be University), Bangalore, India
- Rights
- Restricted Access
- Relation
- ISSN: 8854513; PubMed ID: 38475937; CODEN: BABIE
- Format
- Online
- Language
- English
- Type
- Article
Collection
Citation
Baby Shakila P.; Periasamy T.; Alarfaj A.A.; Arulselvan P.; Ravindran R.; Suriyaprakash J.; Thangavelu I., “Pemetrexed loaded gold nanoparticles as cytotoxic and apoptosis inducers in lung cancer cells through ROS generation and mitochondrial dysfunction pathway,” CHRIST (Deemed To Be University) Institutional Repository, accessed February 25, 2025, https://archives.christuniversity.in/items/show/12995.