Functional characterization of Malabar grouper (Epinephelus malabaricus) interferon regulatory factor 9 involved in antiviral response
- Title
- Functional characterization of Malabar grouper (Epinephelus malabaricus) interferon regulatory factor 9 involved in antiviral response
- Creator
- Periyasamy T.; Ming-Wei L.; Velusamy S.; Ahamed A.; Khan J.M.; Pappuswamy M.; Viswakethu V.
- Description
- IRF9 is a crucial component in the JAK-STAT pathway. IRF9 interacts with STAT1 and STAT2 to form IFN-I-stimulated gene factor 3 (ISGF3) in response to type I IFN stimulation, which promotes ISG transcription. However, the mechanism by which IFN signaling regulates Malabar grouper (Epinephelus malabaricus) IRF9 is still elusive. Here, we explored the nd tissue-specific mRNA distribution of the MgIRF9 gene, as well as its antiviral function in E. malabaricus. MgIRF9 encodes a protein of 438 amino acids with an open reading frame of 1317 base pairs. MgIRF9 mRNA was detected in all tissues of a healthy M. grouper, with the highest concentrations in the muscle, gills, and brain. It was significantly up-regulated by nervous necrosis virus infection and poly (I:C) stimulation. The gel mobility shift test demonstrated a high-affinity association between MgIRF9 and the promoter of zfIFN in vitro. In GK cells, grouper recombinant IFN-treated samples showed a significant response in ISGs and exhibited antiviral function. Subsequently, overexpression of MgIRF9 resulted in a considerable increase in IFN and ISGs mRNA expression (ADAR1, ADAR1-Like, and ADAR2). Co-immunoprecipitation studies demonstrated that MgIRF9 and STAT2 can interact in vivo. According to the findings, M. grouper IRF9 may play a role in how IFN signaling induces ISG gene expression in grouper species. 2024 Elsevier B.V.
- Source
- International Journal of Biological Macromolecules, Vol-266
- Date
- 2024-01-01
- Publisher
- Elsevier B.V.
- Subject
- ADAR families; Epinephelus malabaricus; Interferon; IRF9; Malabar grouper; Nervous necrosis virus
- Coverage
- Periyasamy T., Laboratory of Molecular Virology and Immunology, Department of Aquaculture, The College of Life Science, National Taiwan Ocean University, Keelung, 202, Taiwan, Department of Biotechnology, Nehru Arts and Science College, Tamil Nadu, Coimbatore, 641105, India; Ming-Wei L., Laboratory of Molecular Virology and Immunology, Department of Aquaculture, The College of Life Science, National Taiwan Ocean University, Keelung, 202, Taiwan, Center of Excellence for the Oceans, National Taiwan Ocean University, Keelung, 202, Taiwan; Velusamy S., Department of Biotechnology, Nehru Arts and Science College, Tamil Nadu, Coimbatore, 641105, India; Ahamed A., Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh, 11451, Saudi Arabia; Khan J.M., Department of Food Science and Nutrition, College of Food and Agricultural Sciences, King Saud University, Riyadh, 11451, Saudi Arabia; Pappuswamy M., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bangalore, 560029, India; Viswakethu V., Department of Biotechnology, Nehru Arts and Science College, Tamil Nadu, Coimbatore, 641105, India
- Rights
- Restricted Access
- Relation
- ISSN: 1418130; PubMed ID: 38565369; CODEN: IJBMD
- Format
- Online
- Language
- English
- Type
- Article
Collection
Citation
Periyasamy T.; Ming-Wei L.; Velusamy S.; Ahamed A.; Khan J.M.; Pappuswamy M.; Viswakethu V., “Functional characterization of Malabar grouper (Epinephelus malabaricus) interferon regulatory factor 9 involved in antiviral response,” CHRIST (Deemed To Be University) Institutional Repository, accessed February 25, 2025, https://archives.christuniversity.in/items/show/13139.