Isolation and characterization of polyhydroxyalkanoate producing halotolerant Bacillus subtilis SG1 using marine water samples collected from Calicut coast, Kerala
- Title
- Isolation and characterization of polyhydroxyalkanoate producing halotolerant Bacillus subtilis SG1 using marine water samples collected from Calicut coast, Kerala
- Creator
- Grigary S.; Umesh M.; Mani V.M.
- Description
- Halotolerant bacterial strain isolated from the Calicut coast, Kerala, India, was screened for its potential ability to synthesize polyhydroxyalkanoates (PHA) using Sudan black B and Nile blue staining. The quantitative analysis for PHA production was done in modified M9 media and a PHA yield of about 1.52 g/L was observed with the most potential isolate SG1. Further, the biochemical and molecular characterization of the PHA-producing halotolerant bacteria was done using standard biochemical tests and 16 s ribosomal RNA sequencing respectively and the isolate was identified to be Bacillus subtilis SG1. Further, the PHA recovery was done using solvent extraction method employing acetone and diethyl ether followed by precipitation using chloroform with a maximum PHA yield of 1.52 g/L. Further, the material properties of the extracted polymer were studied using Fourier transform infrared, X-ray diffraction, nuclear magnetic resonance, thermo gravimetric analysis, and differential scanning calorimetry analysis. Further investigations are necessary to optimize PHA production and to carry out its application study in various fields. 2024 Sneha Grigary, et al.
- Source
- Journal of Applied Biology and Biotechnology, Vol-12, No. 2, pp. 282-288.
- Date
- 2024-01-01
- Publisher
- Open Science Publishers LLP Inc.
- Subject
- Bacillus subtilis; Bacterial biopolymer; Halotolerance; Polyhydroxyalkanoates
- Coverage
- Grigary S., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bengaluru, India; Umesh M., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bengaluru, India; Mani V.M., Department of Biotechnology, RVS College of Arts and Science, Tamil Nadu, Coimbatore, India
- Rights
- All Open Access; Gold Open Access
- Relation
- ISSN: 2347212X
- Format
- Online
- Language
- English
- Type
- Article
Collection
Citation
Grigary S.; Umesh M.; Mani V.M., “Isolation and characterization of polyhydroxyalkanoate producing halotolerant Bacillus subtilis SG1 using marine water samples collected from Calicut coast, Kerala,” CHRIST (Deemed To Be University) Institutional Repository, accessed February 25, 2025, https://archives.christuniversity.in/items/show/13238.