Synergistic effects of CuO/TiO2-chitosan-farnesol nanocomposites: Synthesis, characterization, antimicrobial, and anticancer activities on melanoma cells SK-MEL-3
- Title
- Synergistic effects of CuO/TiO2-chitosan-farnesol nanocomposites: Synthesis, characterization, antimicrobial, and anticancer activities on melanoma cells SK-MEL-3
- Creator
- Indumathi T.; Suriyaprakash J.; Alarfaj A.A.; Hirad A.H.; Jaganathan R.; Mathanmohun M.
- Description
- The current investigation focuses on synthesizing copper oxide (CuO)-titanium oxide (TiO2)-chitosan-farnesol nanocomposites with potential antibacterial, antifungal, and anticancer properties against Melanoma cells (melanoma cells [SK-MEL-3]). The nanocomposites were synthesized using the standard acetic acid method and subsequently characterized using an X-ray diffractometer, scanning electron microscope, transmission electron microscopy, and Fourier transform infrared spectroscopy. The results from the antibacterial tests against Streptococcus pneumoniae and Stapylococcus aureus demonstrated significant antibacterial efficacy. Additionally, the antifungal studies using Candida albicans through the agar diffusion method displayed a considerable antifungal effect. For evaluating the anticancer activity, various assays such as MTT assay, acridine orange/ethidium bromide dual staining assay, reactive oxygen species (ROS) generation assay, and mitochondrial membrane potential (MMP) analysis were conducted on SK-MEL-3 cells. The nanocomposites exhibited the ability to induce ROS generation, decrease MMP levels, and trigger apoptosis in SK-MEL-3 cells. Collectively, the findings demonstrated a distinct pattern for the synthesized bimetallic nanocomposites. Furthermore, these nanocomposites also displayed significant (p < 0.05) antibacterial, antifungal, and anticancer effects when tested on the SK-MEL-3 cell line. 2023 Wiley-VCH GmbH.
- Source
- Journal of Basic Microbiology, Vol-64, No. 2
- Date
- 2024-01-01
- Publisher
- John Wiley and Sons Inc
- Subject
- anticancer; farnesol; mitochondrial membrane potential; nanocomposites; ROS; skin cancer
- Coverage
- Indumathi T., Department of Chemistry, CHRIST (Deemed to be University), Karnataka, Bangalore, India; Suriyaprakash J., Guangdong Provincial Key Laboratory of Nanophotonic Functional Materials and Devices, School of Information and Optoelectronic Science and Engineering, South China Normal University, Guangzhou, China; Alarfaj A.A., Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia; Hirad A.H., Department of Botany and Microbiology, College of Science, King Saud University, Riyadh, Saudi Arabia; Jaganathan R., Microbiology Unit, Preclinical Department, Faculty of Medicine, Royal College of Medicine Perak (UniKL-RCMP), Universiti Kuala Lumpur, Ipoh, Malaysia; Mathanmohun M., Department of Microbiology, Muthayammal College of Arts and Science, Rasipuram, Tamil Nadu, Namakkal, India
- Rights
- Restricted Access
- Relation
- ISSN: 0233111X; PubMed ID: 37988658; CODEN: JBMIE
- Format
- Online
- Language
- English
- Type
- Article
Collection
Citation
Indumathi T.; Suriyaprakash J.; Alarfaj A.A.; Hirad A.H.; Jaganathan R.; Mathanmohun M., “Synergistic effects of CuO/TiO2-chitosan-farnesol nanocomposites: Synthesis, characterization, antimicrobial, and anticancer activities on melanoma cells SK-MEL-3,” CHRIST (Deemed To Be University) Institutional Repository, accessed February 24, 2025, https://archives.christuniversity.in/items/show/13326.