Purification and Biochemical Characterization of Beta-Hexosaminidase B from Freshwater CnidarianHydra vulgaris Ind-Pune
- Title
- Purification and Biochemical Characterization of Beta-Hexosaminidase B from Freshwater CnidarianHydra vulgaris Ind-Pune
- Creator
- Kukkemane K.; Krishnapati L.S.; Vuruputuri R.M.; Ranganatha K.S.; Kumar N.S.
- Description
- Beta-N-acetylhexosaminidase (Hex) is a vital lysosomal hydrolase found in all living organisms, playing a crucial role in cellular homeostasis. Dysfunctions in this enzyme are implicated in severe pathological conditions such as Tay-Sachs and Sandhoff diseases in humans. In this paper, we report the purification and biochemical characterization of hexosaminidase from the soluble extracts obtained from the polyps of Hydra vulgaris Ind Pune. The Hydra Hex was purified by two-step sequential chromatography (hydrophobic interaction and gel filtration). Our results suggested that the enzyme isoform purified from Hydra is HexB, most likely to be a homodimer with a subunit mass of 65 kD. The pH optimum was in the range of 5.0 to 6.0 and the temperature optimum in the range of 50 C to 60 C. pH stability and temperature stability were found to be 5.0 and 40C respectively. The homology modelling studies corroborated the homodimeric nature of Hydra HexB, and indicated its structural resemblance to human HexB. This study offers new insights into the biochemical characteristics of Hydra HexB, providing a foundational framework for extensive investigations on this and other lysosomal hydrolases in Hydra. In a broader context, our results significantly contribute to establishing Hydra as a potential model organism to study the lysosomal biogenesis pathway. (2024), (Association of Carbohydrate Chemists and Technologists). All Rights Reserved.
- Source
- Trends in Carbohydrate Research, Vol-16, No. 3, pp. 60-75.
- Date
- 2024-01-01
- Publisher
- Association of Carbohydrate Chemists and Technologists
- Subject
- Cnidarian; Hydra vulgaris; Lysosomal hydrolases; ?-N-Acetylhexosaminidase
- Coverage
- Kukkemane K., Protein Biochemistry and Glycobiology Laboratory, Department of Biochemistry, School of Life Sciences, University of Hyderabad, Telangana, Hyderabad, 500046, India, Department of Life Sciences, School of Sciences, CHRIST University, Karnataka, Bengaluru, 560029, India; Krishnapati L.S., Protein Biochemistry and Glycobiology Laboratory, Department of Biochemistry, School of Life Sciences, University of Hyderabad, Telangana, Hyderabad, 500046, India; Vuruputuri R.M., Protein Biochemistry and Glycobiology Laboratory, Department of Biochemistry, School of Life Sciences, University of Hyderabad, Telangana, Hyderabad, 500046, India; Ranganatha K.S., Protein Biochemistry and Glycobiology Laboratory, Department of Biochemistry, School of Life Sciences, University of Hyderabad, Telangana, Hyderabad, 500046, India; Kumar N.S., Protein Biochemistry and Glycobiology Laboratory, Department of Biochemistry, School of Life Sciences, University of Hyderabad, Telangana, Hyderabad, 500046, India, University of Bremen, Bremen, Germany
- Rights
- Restricted Access
- Relation
- ISSN: 9750304
- Format
- Online
- Language
- English
- Type
- Article
Collection
Citation
Kukkemane K.; Krishnapati L.S.; Vuruputuri R.M.; Ranganatha K.S.; Kumar N.S., “Purification and Biochemical Characterization of Beta-Hexosaminidase B from Freshwater CnidarianHydra vulgaris Ind-Pune,” CHRIST (Deemed To Be University) Institutional Repository, accessed February 23, 2025, https://archives.christuniversity.in/items/show/13447.