ItemArticle

Evidence of microRNAs origination from chloroplast genome and their role in regulating Photosystem II protein N (psbN) mRNA

Title
Evidence of microRNAs origination from chloroplast genome and their role in regulating Photosystem II protein N (psbN) mRNA
Creator
Anand A.; Chauhan S.; Chodon A.; Vimala Kumar K.V.; Saravanakumar S.; Pandi G.
Description
The microRNAs are endogenous, regulating gene expression either at the DNA or RNA level. Despite the availability of extensive studies on microRNA generation in plants, reports on their abundance, biogenesis, and consequent gene regulation in plant organelles remain naVve. Building on previous studies involving pre-miRNA sequencing in Abelmoschus esculentus, we demonstrated that three putative microRNAs were raised from the chloroplast genome. In the current study, we have characterized the genesis of these three microRNAs through a combination of bioinformatics and experimental approaches. The gene sequence for a miRNA, designated as AecpmiRNA1 (A. esculentus chloroplast miRNA), is potentially located in both the genomic DNA, i.e., nuclear and chloroplast genome. In contrast, the gene sequences for the other two miRNAs (AecpmiRNA2 and AecpmiRNA3) are exclusively present in the chloroplast genome. Target prediction revealed many potential mRNAs as targets for AecpmiRNAs. Further analysis using 5N RACE-PCR determined the AecpmiRNA3 binding and cleavage site at the photosystem II protein N (psbN). These results indicate that AecpmiRNAs are generated from the chloroplast genome, possessing the potential to regulate mRNAs arising from chloroplast gene(s). On the other side, the possibility of nuclear genome-derived mRNA regulation by AecpmiRNAs cannot be ruled out. 2024, Termedia Publishing House Ltd.. All rights reserved.
Source
Biotechnologia, Vol-105, No. 1, pp. 19-32.
Date
2024-01-01
Publisher
Termedia Publishing House Ltd.
Subject
chloroplast; miRNA; nuclei; photosystem II protein N; RACE-PCR
Coverage
Anand A., Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, India, Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bengaluru, India; Chauhan S., Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, India; Chodon A., Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, India; Vimala Kumar K.V., Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, India; Saravanakumar S., Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, India; Pandi G., Department of Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, India
Rights
All Open Access; Gold Open Access; Green Open Access
Relation
ISSN: 8607796
Format
Online
Language
English
Type
Article

Collection

Articles

Citation

Anand A.; Chauhan S.; Chodon A.; Vimala Kumar K.V.; Saravanakumar S.; Pandi G., “Evidence of microRNAs origination from chloroplast genome and their role in regulating Photosystem II protein N (psbN) mRNA,” CHRIST (Deemed To Be University) Institutional Repository, accessed April 11, 2025, https://archives.christuniversity.in/items/show/13710.

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