Tissue-Specific Profile and Activity Patterns of Glycosyl Hydrolases from Trichosanthes Anguina (Snake Gourd)
- Title
- Tissue-Specific Profile and Activity Patterns of Glycosyl Hydrolases from Trichosanthes Anguina (Snake Gourd)
- Creator
- Ranganatha K.S.; Kukkemane K.; Kumar N.S.
- Description
- Plant glycosyl hydrolases (GH) and their function have been extensively studied using biochemical and molecular genetic approaches. GHs are involved in metabolism of various glycoconjugates specifically by the hydrolysis of glycosidic bonds and also in N-glycan processing. Several GHs have been extensively characterized from various plant sources and their diverse functional roles in cell wall polysaccharide metabolism, glycan biosynthesis and remodulation, signaling, symbiosis, secondary metabolism, etc. have been studied. However, information on tissue specific distribution of these enzymes, which is crucial for further understanding their physiological roles in plants is highly limited. In these lines, the present study was aimed at qualitative analysis of selected GHs from different tissues of a model plant, Snake Gourd (Trichosanthes anguina). The qualitative analysis of GHs such as ?-mannosidase, ?-hexosaminidase, ?-galactosidase, ?-glucosidase, ?-glucuronidase, ?-glucosidase, ?-galactosidase, ?-mannosidase and ?-fucosidase from seeds, sprouts, roots, stem, leaves, flowers and fruits of the Snake Gourd plant was carried out. Activities of different GHs varied in a tissue specific manner. The ?-mannosidase activity was maximum in ripened fruits whereas ?-hexosaminidase showed highest activity in roots. Interestingly, flowers had maximum activities of ?-glucuronidase and ?-fucosidase. The correlation analysis suggested significant correlations between various GHs which altered in tissue specific manner. (2023) Association of Carbohydrate Chemists and Technologists.
- Source
- Trends in Carbohydrate Research, Vol-15, No. 4, pp. 1-22.
- Date
- 2023-01-01
- Publisher
- Association of Carbohydrate Chemists and Technologists
- Subject
- Chromogenic Assay; Glycosidases; Glycosyl Hydrolases; Snake Gourd; Vegetative Tissue
- Coverage
- Ranganatha K.S., Glycobiology and Protein Biochemistry laboratory, Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, 500046, India; Kukkemane K., Glycobiology and Protein Biochemistry laboratory, Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, 500046, India, Department of Life Sciences, School of Sciences, CHRIST (Deemed to be University), Bengaluru, 560029, India; Kumar N.S., Glycobiology and Protein Biochemistry laboratory, Department of Biochemistry, School of Life Sciences, University of Hyderabad, Hyderabad, 500046, India
- Rights
- Restricted Access
- Relation
- ISSN: 9750304
- Format
- Online
- Language
- English
- Type
- Article
Collection
Citation
Ranganatha K.S.; Kukkemane K.; Kumar N.S., “Tissue-Specific Profile and Activity Patterns of Glycosyl Hydrolases from Trichosanthes Anguina (Snake Gourd),” CHRIST (Deemed To Be University) Institutional Repository, accessed April 25, 2025, https://archives.christuniversity.in/items/show/14448.