Dual-mode chemosensor for the fluorescence detection of zinc and hypochlorite on a fluorescein backbone and its cell-imaging applications
- Title
- Dual-mode chemosensor for the fluorescence detection of zinc and hypochlorite on a fluorescein backbone and its cell-imaging applications
- Creator
- Maity S.; Maity A.C.; Das A.k.; Bhattacharyya N.
- Description
- Fluorescein coupled with 3-(aminomethyl)-4,6-dimethylpyridin-2(1H)-one (FAD) was synthesized for the selective recognition of Zn2+ over other interfering metal ions in acetonitrile/aqueous buffer (1 : 1). Interestingly, there was a significant fluorescence enhancement of FAD in association with Zn2+ at 426 nm by strong chelation-induced fluorescence enhancement (CHEF) without interrupting the cyclic spirolactam ring. A binding stoichiometric ratio of 1 : 2 for the ligand FAD with metal Zn2+ was proven by a Jobs plot. However, the cyclic spirolactam ring was opened by hypochlorite (OCl?) as well as oxidative cleavage of the imine bond, which resulted in the emission enhancement of the wavelength at 520 nm. The binding constant and detection limit of FAD towards Zn2+ were determined to be 1 104 M?1 and 1.79 ?M, respectively, and the detection limit for OCl? was determined as 2.24 ?M. We introduced here a dual-mode chemosensor FAD having both the reactive functionalities for the simultaneous detection of Zn2+ and OCl? by employing a metal coordination (Zn2+) and analytes (OCl?) induced chemodosimetric approach, respectively. Furthermore, for the practical application, we studied the fluorescence imaging inside HeLa cells by using FAD, which demonstrated it can be very useful as a selective and sensitive fluorescent probe for zinc. 2022 The Royal Society of Chemistry.
- Source
- Analytical Methods, Vol-14, No. 28, pp. 2739-2744.
- Date
- 2022-01-01
- Publisher
- Royal Society of Chemistry
- Coverage
- Maity S., Department of Applied Sciences, Haldia Institute of Technology, Hatiberia, West Bengal, Haldia, 721657, India; Maity A.C., Department of Chemistry, Indian Institute of Engineering Science and Technology, Shibpur, West Bengal, Howrah, 711103, India; Das A.k., Department of Chemistry, CHRIST (Deemed to be University), Hosur Road, Karnataka, Bengaluru, 560029, India; Bhattacharyya N., Panskura Banamali College, Purba Medinipur, West Bengal, India
- Rights
- Restricted Access
- Relation
- ISSN: 17599660; PubMed ID: 35775440
- Format
- Online
- Language
- English
- Type
- Article
Collection
Citation
Maity S.; Maity A.C.; Das A.k.; Bhattacharyya N., “Dual-mode chemosensor for the fluorescence detection of zinc and hypochlorite on a fluorescein backbone and its cell-imaging applications,” CHRIST (Deemed To Be University) Institutional Repository, accessed February 23, 2025, https://archives.christuniversity.in/items/show/15049.