PA1 cells containing a truncated DNA polymerase ? protein are more sensitive to gamma radiation
- Title
- PA1 cells containing a truncated DNA polymerase ? protein are more sensitive to gamma radiation
- Creator
- Patra A.; Nag A.; Chakraborty A.; Bhattacharyya N.
- Description
- Purpose: DNA polymerase ? (Pol?) acts in the base excision repair (BER) pathway. Mutations in DNA polymerase ? (Pol?) are associated with different cancers. A variant of Pol? with a 97 amino acid de-letion (Pol??), in heterozygous conditions with wild-type Pol?, was identified in sporadic ovarian tumor samples. This study aims to evaluate the gamma radiation sensitivity of Pol?? for possible target therapy in ovarian cancer treatment. Materials and Methods: Pol?? cDNA was cloned in a GFP vector and transfected in PA1 cells. Stable cells (PA1Pol??) were treated with60Co sourced gamma-ray (015 Gy) to investigate their radiation sensitivity. The affinity of Pol?? with DNA evaluated by DNA protein in silico docking experiments. Results: The result showed a statistically significant (p < 0.05) higher sensitivity towards radiation at different doses (015 Gy) and time-point (4872 hours) for PA1Pol?? cells in comparison with nor-mal PA1 cells. Ten Gy of gamma radiation was found to be the optimal dose. Significantly more PA-1Pol?? cells were killed at this dose than PA1 cells after 48 hours of treatment via an apoptotic pathway. The in silico docking experiments revealed that Pol?? has more substantial binding potential towards the dsDNA than wild-type Pol?, suggesting a possible failure of BER pathway that results in cell death. Conclusion: Our study showed that the PA1Pol?? cells were more susceptible than PA1 cells to gamma radiation. In the future, the potentiality of ionizing radiation to treat this type of cancer will be checked in animal models. 2022 The Korean Society for Radiation Oncology.
- Source
- Radiation Oncology Journal, Vol-40, No. 1, pp. 66-78.
- Date
- 2022-01-01
- Publisher
- Department of Radiation Oncology
- Subject
- DNA polymerase ?; DNA repair; Molecular docking simulation; Ovarian epithelial carcinoma; Radiotherapy
- Coverage
- Patra A., Department of Biotechnology, Panskura Banamali College, West Bengal, India; Nag A., Department of Life Sciences, CHRIST (Deemed to be University), Bangalore, India; Chakraborty A., UGC DAE, Consortium for Scientific Research, Kolkata Centre, Kolkata, India; Bhattacharyya N., Department of Biotechnology, Panskura Banamali College, West Bengal, India
- Rights
- All Open Access; Gold Open Access; Green Open Access
- Relation
- ISSN: 22341900
- Format
- Online
- Language
- English
- Type
- Article
Collection
Citation
Patra A.; Nag A.; Chakraborty A.; Bhattacharyya N., “PA1 cells containing a truncated DNA polymerase ? protein are more sensitive to gamma radiation,” CHRIST (Deemed To Be University) Institutional Repository, accessed February 25, 2025, https://archives.christuniversity.in/items/show/15132.