A protoberberine alkaloid based ratiometric pH-responsive probe for the detection of diabetic ketoacidosis
- Title
- A protoberberine alkaloid based ratiometric pH-responsive probe for the detection of diabetic ketoacidosis
- Creator
- Meenu M.T.; Cherian A.R.; Sherin D.R.; Nair A.R.; Manojkumar T.K.; Radhakrishnan K.V.; Varghese A.
- Description
- Herein we report a ratiometric naturally occurring fluorescent pH probe, berberrubine (BBn) for the direct detection of diabetic ketoacidosis (DKA) conditions of patients having type I diabetes mellitus. The photophysical properties of the probe during pH titrations showed remarkable changes in absorption spectra where two absorption bands at 377 and 326 nm have disappeared followed by the emergence of an absorption maxima at 346 nm in highly acidic conditions. In addition, a fluorescence enhancement effect was observed in the alkaline pH, with a bathochromic shift of 33 nm. Moreover, the solution switches the color from light yellow to light pink with the change of pH from acidic to basic. A pKa value of 7.57 and a good linearity between pH 5.09.0 indicate that the probe can be used efficiently for the DKA condition, where pH variations are in the range of 67. The excellent water solubility, photostability, reversibility, and selectivity of BBn make it a potential pH sensing agent for acidic microenvironments. The reversible sensing of pH variations during DKA could be effective in primary detection and diagnosis which can assist in avoiding further complications of acidosis. 2021 Elsevier Ltd
- Source
- Dyes and Pigments, Vol-194
- Date
- 2021-01-01
- Publisher
- Elsevier Ltd
- Subject
- Berberrubine; Diabetic ketoacidosis; Fluorescent probe; pH sensor; Ratiometric
- Coverage
- Meenu M.T., Chemical Sciences and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST), Thiruvananthapuram, 695019, India, Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India; Cherian A.R., Department of Chemistry, CHRIST (Deemed to be University), Hosur Road, Bengaluru, 560029, India; Sherin D.R., School of Digital Sciences, Kerala University of Digital Sciences, Innovation and Technology, Thiruvananthapuram, 695317, India; Nair A.R., Chemical Sciences and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST), Thiruvananthapuram, 695019, India; Manojkumar T.K., School of Digital Sciences, Kerala University of Digital Sciences, Innovation and Technology, Thiruvananthapuram, 695317, India; Radhakrishnan K.V., Chemical Sciences and Technology Division, CSIR-National Institute for Interdisciplinary Science and Technology (CSIR-NIIST), Thiruvananthapuram, 695019, India, Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, 201002, India; Varghese A., Department of Chemistry, CHRIST (Deemed to be University), Hosur Road, Bengaluru, 560029, India
- Rights
- Restricted Access
- Relation
- ISSN: 1437208; CODEN: DYPID
- Format
- Online
- Language
- English
- Type
- Article
Collection
Citation
Meenu M.T.; Cherian A.R.; Sherin D.R.; Nair A.R.; Manojkumar T.K.; Radhakrishnan K.V.; Varghese A., “A protoberberine alkaloid based ratiometric pH-responsive probe for the detection of diabetic ketoacidosis,” CHRIST (Deemed To Be University) Institutional Repository, accessed February 25, 2025, https://archives.christuniversity.in/items/show/15590.