Electrochemical sensing of vitamin B12 deficiency marker methylmalonic acid using PdAu-PPy tailored carbon fiber paper electrode
- Title
- Electrochemical sensing of vitamin B12 deficiency marker methylmalonic acid using PdAu-PPy tailored carbon fiber paper electrode
- Creator
- Akshaya K.B.; Anitha V.; Nidhin M.; Sudhakar Y.N.; Louis G.
- Description
- Vitamin B12 is very important for human metabolism and its deficiency can cause anemia and the production of large red blood cells. An increased concentration of methylmalonic acid (MMA) is detected much before the transformation of blood cells, which thereby is an early indicator for mild or serious Vitamin B12 deficiency. A simple electrochemical sensor based on PalladiumGold (PdAu) was developed by electrodeposition of PdAu nanoparticles on Polypyrrole (PPy) modified carbon fiber paper (CFP) electrode. The modified electrodes were characterized by High resolution transmission electron microscopy (HRTEM), Field emission scanning electron microscopy (FESEM) with energy-dispersive X-ray spectroscopy (EDS), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and electroanalytical techniques. Differential Pulse Voltammetric (DPV) studies have established that under optimum conditions, the developed sensor exhibits a broad linear dynamic range (4.01 pM - 52.5 nM) with a very low detection limit (1.32 pM). The proposed method was effectively applied in the non-enzymatic determination of MMA at an ultralow level in human blood serum and urine samples. The method displayed high selectivity toward MMA in the presence of other interfering substances. 2020 Elsevier B.V.
- Source
- Talanta, Vol-217
- Date
- 2020-01-01
- Publisher
- Elsevier B.V.
- Subject
- Electrochemical; Human blood serum; Methylmalonic acid (MMA); PdAu nanoparticles; Polypyrrole
- Coverage
- Akshaya K.B., Department of Chemistry, CHRIST (Deemed to be University), Hosur Road, Bengaluru, 560029, India; Anitha V., Department of Chemistry, CHRIST (Deemed to be University), Hosur Road, Bengaluru, 560029, India; Nidhin M., Department of Chemistry, CHRIST (Deemed to be University), Hosur Road, Bengaluru, 560029, India; Sudhakar Y.N., Department of Chemistry, CHRIST (Deemed to be University), Hosur Road, Bengaluru, 560029, India; Louis G., Department of Chemistry, CHRIST (Deemed to be University), Hosur Road, Bengaluru, 560029, India
- Rights
- Restricted Access
- Relation
- ISSN: 399140; PubMed ID: 32498897; CODEN: TLNTA
- Format
- Online
- Language
- English
- Type
- Article
Collection
Citation
Akshaya K.B.; Anitha V.; Nidhin M.; Sudhakar Y.N.; Louis G., “Electrochemical sensing of vitamin B12 deficiency marker methylmalonic acid using PdAu-PPy tailored carbon fiber paper electrode,” CHRIST (Deemed To Be University) Institutional Repository, accessed February 24, 2025, https://archives.christuniversity.in/items/show/16224.