Assessment of microsatellite instability for screening bladder cancer in high-risk population
- Title
- Assessment of microsatellite instability for screening bladder cancer in high-risk population
- Creator
- Wadhwa N.; Mathew B.; Tandon S.; Biju V.; Tiwari A.
- Description
- Aims: This study aims to determine the diagnostic efficacy of microsatellite markers for screening bladder cancer in population at high risk. Materials and Methods: A population of 200 people was screened for bladder cancer using a set of microsatellite markers. Urine samples were obtained from four different types of population groups - Group 1 (healthy population group), Group 2 (current smokers with a smoking history of more than 10 years), Group 3 (bladder cancer group), and Group 4 (bladder cancer group who were former smokers with a history of more than 10 years). Polymerase chain reaction (PCR) was performed to amplify microsatellite sequences at D9S63, D9S156, and D9S283. PCR products were separated on 1.8% agarose gel and were scanned using ultraviolet transilluminator. Results: In Group 2 (high-risk population group, mainly current smokers with a history of more than 10 years), microsatellite alterations were found in 36 out of 50 people. We observed microsatellite alterations in 38 out of 50 people in Group 3 (bladder cancer group) and in 39 out of 50 people in Group 4 (bladder cancer group, mainly former smokers with a history of more than 10 years). The sensitivity of this test in Group 2, Group 3, and Group 4 was found to be 72%, 76% and 78%, respectively. The specificity of this test in each group was found to be 90%. Conclusion: Using these set of microsatellite markers, medium sensitivity and high specificity were reported for this test. The current findings suggest that a set of microsatellite markers (D9S63, D9S156, and D9S283) can be used to detect bladder cancer in high-risk population. 2018 Medknow Publications. All Rights Reserved.
- Source
- Journal of Cancer Research and Therapeutics, Vol-14, No. 5, pp. 916-920.
- Date
- 2018-01-01
- Publisher
- Wolters Kluwer Medknow Publications
- Subject
- Bladder cancer; microsatellite instability; microsatellite markers
- Coverage
- Wadhwa N., School of Biotechnology, Rajiv Gandhi Proudyogiki Vishwavidyalaya, State Technological University of Madhya Pradesh, Airport Bypass Road, Bhopal, Madhya Pradesh, 462 033, India; Mathew B., School of Biotechnology, Rajiv Gandhi Proudyogiki Vishwavidyalaya, State Technological University of Madhya Pradesh, Airport Bypass Road, Bhopal, Madhya Pradesh, 462 033, India; Tandon S., Jawaharlal Nehru Cancer Hospital and Research Centre, Idgah Hills, Bhopal, Madhya Pradesh, India; Biju V., Department of Computer Science and Engineering, Christ University, Kanmaike, Kumbalagodu, Bengaluru, Karnataka, India; Tiwari A., School of Biotechnology, Rajiv Gandhi Proudyogiki Vishwavidyalaya, State Technological University of Madhya Pradesh, Airport Bypass Road, Bhopal, Madhya Pradesh, 462 033, India
- Rights
- All Open Access; Gold Open Access
- Relation
- ISSN: 9731482; PubMed ID: 30197325
- Format
- Online
- Language
- English
- Type
- Article
Collection
Citation
Wadhwa N.; Mathew B.; Tandon S.; Biju V.; Tiwari A., “Assessment of microsatellite instability for screening bladder cancer in high-risk population,” CHRIST (Deemed To Be University) Institutional Repository, accessed February 24, 2025, https://archives.christuniversity.in/items/show/16859.