Detection of carbapenem resistance genes and cephalosporin, and quinolone resistance genes along with oqxAB gene in Escherichia coli in hospital wastewater: A matter of concern
- Title
- Detection of carbapenem resistance genes and cephalosporin, and quinolone resistance genes along with oqxAB gene in Escherichia coli in hospital wastewater: A matter of concern
- Creator
- Chandran S.P.; Diwan V.; Tamhankar A.J.; Joseph B.V.; Rosales-Klintz S.; Mundayoor S.; Lundborg C.S.; Macaden R.
- Description
- Aims: This study was performed to detect the presence of Escherichia coli resistant to cephalosporins, carbapenems and quinolones in hospital wastewater. Methods and Results: Wastewaters from a rural (H1) and an urban (H2) hospital were tested for E.coli resistant to cephalosporins, carbapenem and quinolones. Genes coding for chromosomal and plasmid-mediated resistance and phylogenetic grouping was detected by multiplex polymerase chain reaction (PCR) and for genetic relatedness by rep-PCR. Of 190 (H1=94; H2=96) E.coli examined, 44% were resistant to both cephalosporins and quinolones and 3% to imipenem. ESBLs were detected phenotypically in 96% of the isolates, the gene blaCTX-M coding for 87% and blaTEM for 63%. Quinolone resistance was due to mutations in gyrA and parC genes in 97% and plasmid-coded aac-(6?)-Ib-cr in 89% of isolates. Only in one carbapenem-resistant E.coli, NDM-1 was detected. Nearly 67% of the isolates belonged to phylogenetic group B2. There was no genetic relatedness among the isolates. Conclusions: Hospital wastewater contains genetically diverse multidrug-resistant E.coli. Significance and Impact of the Study: This study stresses the need for efficient water treatment plants in healthcare settings as a public health measure to minimize spread of multidrug-resistant bacteria into the environment. 2014 The Society for Applied Microbiology.
- Source
- Journal of Applied Microbiology, Vol-117, No. 4, pp. 984-995.
- Date
- 2014-01-01
- Subject
- Carbapenem resistance; Cephalosporin resistance; Hospital wastewater; Multidrug-resistant E.coli; Quinolones resistance
- Coverage
- Chandran S.P., Division of Infectious Diseases, St. John's Research Institute, Bangalore, India, Department of Microbiology, St. John's Medical College, Bangalore, India; Diwan V., Department of Public Health Sciences, Global Health/IHCAR, Karolinska Institutet, Stockholm, Sweden, Department of Public Health and Environment, R.D. Gardi Medical College, Ujjain, India, International Center for Health Research, R.D. Gardi Medical College, Ujjain, India; Tamhankar A.J., Department of Public Health Sciences, Global Health/IHCAR, Karolinska Institutet, Stockholm, Sweden, Department of Environmental Medicine, Indian Initiative for Management of Antibiotic Resistance (IIMAR), R D Gardi Medical College, Ujjain, India; Joseph B.V., Departments of Biotechnology, Christ University, Bangalore, India; Rosales-Klintz S., Department of Public Health Sciences, Global Health/IHCAR, Karolinska Institutet, Stockholm, Sweden; Mundayoor S., Mycobacterial Research Group, Department of Molecular Microbiology, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, India; Lundborg C.S., Department of Public Health Sciences, Global Health/IHCAR, Karolinska Institutet, Stockholm, Sweden; Macaden R., Division of Infectious Diseases, St. John's Research Institute, Bangalore, India
- Rights
- Restricted Access
- Relation
- ISSN: 13645072; PubMed ID: 24975198; CODEN: JAMIF
- Format
- Online
- Language
- English
- Type
- Article
Collection
Citation
Chandran S.P.; Diwan V.; Tamhankar A.J.; Joseph B.V.; Rosales-Klintz S.; Mundayoor S.; Lundborg C.S.; Macaden R., “Detection of carbapenem resistance genes and cephalosporin, and quinolone resistance genes along with oqxAB gene in Escherichia coli in hospital wastewater: A matter of concern,” CHRIST (Deemed To Be University) Institutional Repository, accessed February 24, 2025, https://archives.christuniversity.in/items/show/17283.