Computationally efficient wavelet domain solver for florescence diffuse optical tomography

Title

Computationally efficient wavelet domain solver for florescence diffuse optical tomography

Creator

Francis K.J.; Jose I.

Description

Estrogen induced proliferation of mutant cells is a growth signal hallmark of breast cancer. Fluorescent molecule that can tag Estrogen Receptor (ER) can be effectively used for detecting cancerous tissue at an early stage. A novel targetspecific NIRf dye conjugate aimed at measuring ER status was synthesized by ester formation between 17-? estradiol and a hydrophilic derivative of ICG, cyanine dye, bis-1,1-(4-sulfobutyl) indotricarbocyanine-5-carboxylic acid, sodium salt. In-vitro studies provided specific binding on ER+ve [MCF-7] cells clearly indicating nuclear localization of the dye for ER+ve as compared to plasma level staining for MDAMB-231. Furthermore, cancer prone cells showed 4.5-fold increase in fluorescence signal intensity compared to control.; A model of breast phantom was simulated to study the in-vivo efficiency of dye with the parameters of dye obtained from photo-physical and in-vitro studies. The excitation (754 nm) and emission (787 nm) equation are solved independently using parallel processing strategies. The results were obtained by carrying out wavelet transformation on forward and the inverse data sets. An improvisation of the Information content of system matrix was suggested in wavelet domain. The inverse problem was addressed using LevenbergMarquardt (LM) procedure with the minimization of objective function using Tikhonov approach. The multi resolution property of wavelet transform was explored in reducing error and increasing computational efficiency. Our results were compared with the single resolution approach on various parameters like computational time, error function, and Normalized Root Mean Square (NRMS) error. A model with background absorption coefficient of 0.01 mm-1 with anomalies of 0.02 mm-1 with constant reduced scattering of 2.0 mm for different concentration of dye was compared in the result. The reconstructed optical properties were in concurrence with the tissue property at 787 nm. We intend our future plans on in-vivo study on developing a complete instrumentation for imaging a target specific lipophilic dye. Springer International Publishing Switzerland 2014.

Source

Springer Proceedings in Mathematics and Statistics, Vol-92, pp. 457-469.

Date

2014-01-01

Publisher

Springer New York LLC

Subject

Estrogen receptor; Inverse solving; Multi-resolution; NIRf dye; Parallel processing; Wavelet transform

Coverage

Francis K.J., Christ University, Bangalore, 560060, Karnataka, India; Jose I., Christ University, Bangalore, 560060, Karnataka, India

Rights

Restricted Access

Relation

ISSN: 21941009; ISBN: 978-331908104-5

Format

Online

Language

English

Type

Conference paper

Identifier

https://doi.org/10.1007/978-3-319-08105-2__31

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