ItemArticle

Biogenic Copper Oxide@rGO Nanocomposite for Decontamination of a Food Threat B. Cereus in a Rice Model

Title
Biogenic Copper Oxide@rGO Nanocomposite for Decontamination of a Food Threat B. Cereus in a Rice Model
Creator
Pamshong, Mercyland; Kharthangmaw, Ibansaralang; Saji, Janita; Dubey, Vikas; Alanazi, Amer M.; Ali Khan, Azmat; Haldorai, Yuvaraj; Shukla, Shruti
Description
Pathogenic microorganisms have become a serious threat to human beings all over the world as they cause severe disease illnesses. This study used supercritical carbon dioxide as a green solvent to prepare a nanocomposite composed of copper oxide (CuO) nanoparticles dispersed on the surface of reduced graphene oxide (rGO). The resulting nanocomposite was examined using a various of alanytical techniques. The developed CuO/rGO nanocomposite exhibited potenail antimicrobial ability against a food pathogenic bacterium, Bacillus cereus. Additionality, it showed high toxicity towards B. cereus, confirmed by the means of fluorescent live-dead counting of cells. Its mechanistic role against a food meneac was confirmed by the means of time-kill ability (complete inhibition at 200min), cell membrane integrity (OD of control: 1.62; CuO/rGO: 3.78 at 60min), membrane uptake (Control: 27.21%; Cuo/rGO: 64.33%), and membrane potential ability (Control: 58.33; CuO/rGO: 24.12) towards B. cereus. Study of scanning electron microscopy analyse resulted in the membrane disruption of B. cereus by the nanocomposite. Moreover, the CuO/rGO nanocomposite inhibited in vitro biofilm formation ability (Crystal violet uptake - Control: 31.33%; CuO/rGO: 59.11%) of B. cereus. Furthermore, the nanocomposite coating was used as a rinse solution for rice bowl packages. Interestingly, a rinsing solution (20% and 4060min) significantly inhibited the CFU count of B. cereus in rice by 2.1 log cfu/g as compaered to control 6.2 log cfu/g. The outcomes highlight the effectiveness of nanocomposite coating against food menace B. cereus, suggesting that the developed nanocomposite could be applied as an effective antimicrobial marinade and/or a rinse for raw rice preservation aginst hazardous foodborne pathogens. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature 2026.
Source
BioNanoScience;Volume;16;Issue;5;Article No.;318;
Date
01-01-2026
Publisher
Springer
Subject
Antimicrobial; Bacillus cereus; Biofilm inhibition; Copper oxide, nanocomposite; Food test model
Coverage
Pamshong M., Department of Nanotechnology, North-Eastern Hill University (NEHU), East Khasi Hills, Meghalaya, Shillong, 793022, India; Kharthangmaw I., Department of Nanotechnology, North-Eastern Hill University (NEHU), East Khasi Hills, Meghalaya, Shillong, 793022, India; Saji J., Department of Sciences and Humanities, CHRIST University, Bengaluru, 560074, India; Dubey V., Department of Physics, North-Eastern Hill University (NEHU), East Khasi Hills, Meghalaya, Shillong, 793022, India; Alanazi A.M., Pharmaceutical Biotechnology Laboratory, Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh, 11451, Saudi Arabia; Ali Khan A., Pharmaceutical Biotechnology Laboratory, Department of Pharmaceutical Chemistry, College of Pharmacy, King Saud University, Riyadh, 11451, Saudi Arabia; Haldorai Y., Department of Chemistry (SF), PSG College of Arts and Science, Tamil Nadu, Coimbatore, 641014, India; Shukla S., Department of Nanotechnology, North-Eastern Hill University (NEHU), East Khasi Hills, Meghalaya, Shillong, 793022, India
Rights
Restricted Access; Hardcopy may be available in the library
Relation
ISSN: 21911630;
Format
online
Language
English
Type
Article

Citation

Pamshong, Mercyland; Kharthangmaw, Ibansaralang; Saji, Janita; Dubey, Vikas; Alanazi, Amer M.; Ali Khan, Azmat; Haldorai, Yuvaraj; Shukla, Shruti, “Biogenic Copper Oxide@rGO Nanocomposite for Decontamination of a Food Threat B. Cereus in a Rice Model,” CHRIST (Deemed To Be University) Institutional Repository, accessed June 20, 2026, https://archives.christuniversity.in/items/show/22020.

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