ItemArticle

Effects of light-emitting diode (LED) light sources on in vitro protocorm-like body (PLB) proliferation, plantlet regeneration, and ex vitro acclimatization in Cymbidium Snow Pearl

Title
Effects of light-emitting diode (LED) light sources on in vitro protocorm-like body (PLB) proliferation, plantlet regeneration, and ex vitro acclimatization in Cymbidium Snow Pearl
Creator
Kang, Beomsoo; Kim, Ye-Won; Lee, Han-Sol; Han, Jong-Eun; Murthy, Hosakatte Niranjana; Kim, Yae Jin; Park, Pil Man; Park, So-Young
Description
One of the key environmental elements that influences plant growth in vitro is light quality. Currently, a variety of horticultural plants are regenerated in vitro using light-emitting diode (LED) light sources to produce healthy, high-quality plants that can adapt well to ex vitro transplantation conditions. Investigating the impact of various spectrum light sources at various phases of in vitro regeneration is essential, though. The objective of this research was to examine how red (R), blue (B), white (W), red plus blue (RB, 1:1), red, green (G), and blue (RGB, 1:1:1) LEDs affect the growth of protocorm-like bodies (PLBs), shoot regeneration, and the rooting stages of shoots. The findings showed that B-LEDs were accountable for PLB proliferation, whereas R-LEDs were responsible for increased shoot regeneration and improved growth matrices with shoots and plantlets as compared to other LED treatments. Plant height, leaf count, and dry matter percentage were all higher in the plantlets that were regenerated under R-LED. On the other hand, more root regeneration and longer roots were caused by the B-LED treatment. Plants cultivated under RB LEDs had greater levels of carotenoid pigments, total chlorophyll, chlorophyll a, and chlorophyll b. When compared to other treatments, photosynthetic fluorescence characteristics like maximum quantum yield of PSII (Fv/Fm), photochemical quenching coefficient (qP), relative electron transport in PSII (ETRII), and non-photochemical quenching (NPQ) were lower in plants cultivated under R-LED treatment. The best LED for in vitro Cymbidium Snow Pearl plant regeneration was as follows: The B-LED was good for PLB proliferation, the R-LED was appropriate during shoot regeneration, and the growth of plantlets, the physiological characteristics such as chlorophyll a, chlorophyll b, total chlorophyll, and carotenoid content and number of epidermal cells per unit area were optimum with the plants grown under RB LED light. The Author(s), under exclusive licence to Korean Society for Horticultural Science 2026.
Source
Horticulture Environment and Biotechnology;
Date
01-01-2026
Publisher
Springer
Coverage
Kang B., Department of Horticultural Science, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, South Korea; Kim Y.-W., Department of Horticultural Science, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, South Korea; Lee H.-S., Department of Horticultural Science, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, South Korea; Han J.-E., Department of Horticultural Science, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, South Korea, Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), 181 Ipsin-gil, Jeonbuk, 56212, South Korea; Murthy H.N., Department of Horticultural Science, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, South Korea, Department of Life Sciences, Christ University, Bengaluru, 560029, India; Kim Y.J., National Institute of Horticultural and Herbal Science, Rural Development Administration, 385 Seodun-ro, Gyeonggi-do, Suwon-si, South Korea; Park P.M., National Institute of Horticultural and Herbal Science, Rural Development Administration, 385 Seodun-ro, Gyeonggi-do, Suwon-si, South Korea; Park S.-Y., Department of Horticultural Science, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, South Korea
Rights
Restricted Access; Hardcopy may be available in the library
Relation
ISSN: 22113452;
Format
online
Language
English
Type
Article

Citation

Kang, Beomsoo; Kim, Ye-Won; Lee, Han-Sol; Han, Jong-Eun; Murthy, Hosakatte Niranjana; Kim, Yae Jin; Park, Pil Man; Park, So-Young, “Effects of light-emitting diode (LED) light sources on in vitro protocorm-like body (PLB) proliferation, plantlet regeneration, and ex vitro acclimatization in Cymbidium Snow Pearl,” CHRIST (Deemed To Be University) Institutional Repository, accessed June 17, 2026, https://archives.christuniversity.in/items/show/22051.

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