ItemArticle

Effects of citric acid, ascorbic acid, and polyvinylpyrrolidone in overcoming medium browning during micropropagation of Phalaenopsis univivace

Title
Effects of citric acid, ascorbic acid, and polyvinylpyrrolidone in overcoming medium browning during micropropagation of Phalaenopsis univivace
Creator
Kang, Beomsoo; Kim, Ye-Won; Murthy, Hosakatte Niranjana; Ho, Thanh-Tam; Yoon, Yeo-Joong; An, Hae-Ryun; Lee, Su-Young; Park, So-Young
Description
The most significant issues in the early phases of plant tissue culture are the browning of the explant and media. The objective of this research was to determine how well citric acid (CTR, 20 and 40 mg L? 1), ascorbic acid (ASA, 200 and 400 mg L? 1), and polyvinylpyrrolidone (PVP, 20 and 40 mg L? 1) could help Phalaenopsis Univivace overcome medium browning during the stages of shoot multiplication (MS medium supplemented with 150 mL L? 1 coconut water, 10 mg L? 1 adenine sulfate, 1 mg L? 1 thidiazuron, and 15g L? 1 sucrose) and rooting of shoots (MS medium with 25 mL L? 1 coconut water, 1 mg L? 1 indole butyric acid, 15g L? 1 sucrose). Numerous metrics were estimated, including the number of shoots that were regenerated during the shoot regeneration stage, the number of roots that were regenerated during the shoot rooting stage, and a number of growth parameters. The quantity of carotenoid and chlorophyll pigments, PSII quantum yield (Fv/Fm), the effective PSII quantum yield (YII), and the non-photochemical quenching (NPQ) were also measured. Additionally, oxidative stress enzyme malonaldehyde (MDA) and preventive antioxidant enzymes including catalase (CAT), peroxidase (POD), and superoxide dismutase (SOD) were measured in regenerated shoots and plantlets. The results showed that the medium browning issues during the shoot and root regeneration stages were resolved by supplementing with CTR, ASA, and PVP. In terms of shoot regeneration, rooting of shoots, and enhancing shoot and plantlet growth metrics, 40 mg L? 1 CTR supplementation was determined to be superior overall. The Author(s), under exclusive licence to Korean Society for Horticultural Science 2025.
Source
Horticulture Environment and Biotechnology;Volume;66;Issue;5;pp.1175-1187
Date
01-01-2025
Publisher
Springer
Subject
Adsorbent; Antioxidants; In vitro regeneration; Orchid; Tissue culture
Coverage
Kang B., Division of Animal, Horticultural and Food Sciences, Department of Horticultural Science, Chungbuk National University, Cheongju, 28644, South Korea; Kim Y.-W., Division of Animal, Horticultural and Food Sciences, Department of Horticultural Science, Chungbuk National University, Cheongju, 28644, South Korea; Murthy H.N., Division of Animal, Horticultural and Food Sciences, Department of Horticultural Science, Chungbuk National University, Cheongju, 28644, South Korea, Department of Life Sciences, CHRIST (Deemed to be University), Bengaluru, 560029, India; Ho T.-T., Institute for Global Health Innovations, Duy Tan University, Hoa Khanh Nam Campus 120 Hoang Minh Thao, Da Nang, 550000, Viet Nam; Yoon Y.-J., YooniPlant Tech Co., Umseong, Chungbuk, 27660, South Korea; An H.-R., Floriculture Research Division, National Institute of Horticultural & Herbal Science, Wanju, 55365, South Korea; Lee S.-Y., Floriculture Research Division, National Institute of Horticultural & Herbal Science, Wanju, 55365, South Korea; Park S.-Y., Division of Animal, Horticultural and Food Sciences, Department of Horticultural Science, Chungbuk National University, Cheongju, 28644, South Korea
Rights
Restricted Access; Hardcopy may be available in the library
Relation
ISSN: 22113452;
Format
online
Language
English
Type
Article

Citation

Kang, Beomsoo; Kim, Ye-Won; Murthy, Hosakatte Niranjana; Ho, Thanh-Tam; Yoon, Yeo-Joong; An, Hae-Ryun; Lee, Su-Young; Park, So-Young, “Effects of citric acid, ascorbic acid, and polyvinylpyrrolidone in overcoming medium browning during micropropagation of Phalaenopsis univivace,” CHRIST (Deemed To Be University) Institutional Repository, accessed June 17, 2026, https://archives.christuniversity.in/items/show/22050.

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