In vitro storage under slow growth, plant regeneration, and ex vitro acclimatization of Ligusticum officinale (Makino) Kitag
- Title
- In vitro storage under slow growth, plant regeneration, and ex vitro acclimatization of Ligusticum officinale (Makino) Kitag
- Creator
- Kim, Ye-Won; Lee, Han-Sol; Murthy, Hosakatte Niranjana; Lee, Hyoshin; Park, So-Young
- Description
- Ligusticum officinale is an important medicinal plant belonging to Apiaceae. It does not set seeds and is propagated by rhizome division. However, its sensitivity to high summer temperatures makes field cultivation and genetic resource conservation challenging. To conserve L. officinale germplasm, we employed an in vitro slow-growth storage (SGS) method. Shoot cultures of L. officinale were established on Murashige and Skoog medium supplemented with 1.0 mg/L benzyl adenine, 30 g/L sucrose, and 2.4 g/L gelrite. Cultures were kept for one, three, five, and seven months. The effects of storage temperatures of 25 C (control) versus 15 C, medium supplementation with or without mannitol (3%), and abscisic acid (ABA), 0.5 mg/L, were examined. At the conclusion of the conservation period, survival was measured right away. Four weeks later, the shoot proliferation medium was used to measure the regrowth rate and recovery features. Subsequently, the regenerated shoots were transferred to MS medium supplemented with 1.0 mg/L indole-3-butyric acid for rooting of shoots for 4 weeks. The findings showed that even after seven months, shoot cultures kept at 15 C with medium supplemented with 3% mannitol and 0.5 mg/L ABA maintained a good survival rate of 83.3%. When compared to the control, most growth indices, including shoot length, fresh weight, number of shoots, and number of leaves, were significantly suppressed by mannitol and ABA combined treatment. A regrowth rate of 71% was achieved after transfer to proliferation medium. All the shoots that were cultured on rooting medium involved in rooting and plantlets were successfully acclimatized in controlled conditions. 2026 SAAB. Published by Elsevier B.V. All rights are reserved, including those for text and data mining, AI training, and similar technologies.
- Source
- South African Journal of Botany;Volume;195;pp.152-159
- Date
- 01-01-2026
- Publisher
- Elsevier B.V.
- Subject
- In vitroconservation; Ligusticum officinale; Medicinal plant; Shoot proliferation; Slow growth storage
- Coverage
- Kim Y.-W., Department of Horticultural Science, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, South Korea; Lee H.-S., Department of Horticultural Science, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, South Korea; Murthy H.N., Department of Horticultural Science, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, South Korea, Department of Life Sciences, Christ University, Bengaluru, 560029, India; Lee H., Department of Horticultural Science, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, South Korea; Park S.-Y., Department of Horticultural Science, Division of Animal, Horticultural and Food Sciences, Chungbuk National University, Cheongju, 28644, South Korea
- Rights
- Restricted Access; Hardcopy may be available in the library
- Relation
- ISSN: 2546299; CODEN: SAJBD
- Format
- online
- Language
- English
- Type
- Article
Collection
Citation
Kim, Ye-Won; Lee, Han-Sol; Murthy, Hosakatte Niranjana; Lee, Hyoshin; Park, So-Young, “In vitro storage under slow growth, plant regeneration, and ex vitro acclimatization of Ligusticum officinale (Makino) Kitag,” CHRIST (Deemed To Be University) Institutional Repository, accessed June 18, 2026, https://archives.christuniversity.in/items/show/22452.