ItemArticle

Engineered MOF-199 Modified Electrodes for Enhanced Electrochemical Immunosensing of Lactoferrin via Signal Amplification

Title
Engineered MOF-199 Modified Electrodes for Enhanced Electrochemical Immunosensing of Lactoferrin via Signal Amplification
Creator
Harilal, Calvin Carl; Uwaya, Gloria Ebube; Kanchi, Suvardhan; Bisetty, Krishna
Description
The detection of lactoferrin (LF), an essential immunological and nutritional biomarker, demands highly sensitive analytical platforms for low-level detection. In this study, a label-free electrochemical immunosensing platform was constructed by immobilizing anti-lactoferrin antibodies (Anti-LF-Ab) on a multiwalled carbon nanotube and a metalorganic framework (MWCNT/MOF-199) nanocomposite-modified glassy carbon electrode (GCE). The synergy between MWCNTs and MOF-199 provided abundant active sites for antibody immobilization and enhanced electron transfer, yielding an eightfold current increase compared to bare GCE. Electrochemical analyses confirmed efficient charge transfer and stable antibody binding. Under optimized conditions, the immunosensor exhibited exceptional analytical performance with a low detection limit of 5.24 ng ml?1 and a quantification limit of 17.46 ng ml?1 across a wide detection range of 050 ng ml?1. The platform demonstrated strong analytical reliability, including excellent repeatability (RSD < 5%), reproducibility and operational stability over multiple measurement cycles for LF detection in food diagnostics. In addition, Monte Carlo simulations confirmed the stability of the layer-by-layer assembly, supporting the robustness of the engineered sensing interface. 2025 The Author(s). Published on behalf of The Electrochemical Society by IOP Publishing Limited.
Source
Journal of the Electrochemical Society;Volume;172;Issue;12;Article No.;126503;
Date
01-01-2025
Publisher
Institute of Physics
Subject
electrochemical immunosensor; lactoferrin; metal-organic frameworks
Coverage
Harilal C.C., Department of Chemistry, Faculty of Applied Sciences, Durban University of Technology, Durban, 4000, South Africa; Uwaya G.E., Department of Chemistry, Faculty of Applied Sciences, Durban University of Technology, Durban, 4000, South Africa; Kanchi S., Department of Chemistry, Bengaluru, 560029, India, Centre for Renewable Energy and Environmental Sustainability, CHRIST University, Bengaluru, 560 029, India; Bisetty K., Department of Chemistry, Faculty of Applied Sciences, Durban University of Technology, Durban, 4000, South Africa
Rights
All Open Access; Hybrid Gold Open Access
Relation
ISSN: 134651; CODEN: JESOA
Format
online
Language
English
Type
Article

Citation

Harilal, Calvin Carl; Uwaya, Gloria Ebube; Kanchi, Suvardhan; Bisetty, Krishna, “Engineered MOF-199 Modified Electrodes for Enhanced Electrochemical Immunosensing of Lactoferrin via Signal Amplification,” CHRIST (Deemed To Be University) Institutional Repository, accessed June 18, 2026, https://archives.christuniversity.in/items/show/23059.

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