ItemArticle

Development of a Thin Layer Chromatography method to detect the presence of spiked Steroid drugs in Herbal Crude extracts

Title
Development of a Thin Layer Chromatography method to detect the presence of spiked Steroid drugs in Herbal Crude extracts
Creator
Rithu, K.; Gayathri, Jayasankar; Nag, Anish
Description
This study aimed to develop a better, faster and more efficient TLC method for detecting steroids in the herbal samples. The steroid drugs dexamethasone and prednisolone were considered possible adulterants and were spiked into herbal extracts of neem and amla. Optimization of the TLC mobile phases for better resolution for prominent visualisation of spots was performed with several combinations of solventsnamely, methanol, chloroform, ethyl acetate, toluene, ethanol, isopropyl alcohol, acetic acid and petroleum ether and, spray reagents such as iodine, potassium permanganate, sulphuric acid and, methanol. The optimal result was obtained using chloroform and methanol (9:1, v/v) as the mobile phase and methanol-sulphuric acid (9:1, v/v) as the spraying agent. The method was also able to separate a mixture of dexamethasone and prednisolone in the ratio of 1:1. The retention factors (Rf) for the steroids dexamethasone and prednisolone were within the range of 0.50-0.55 and 0.73-0.83 respectively. The lowest detection limit for the steroid drugs when mixed with herbal samples (neem and amla) was 0.1 g/ml. The developed TLC method is robust and can be conveniently utilised for detecting steroid adulterants in herbal samples. 2026, World Researchers Associations. All rights reserved.
Source
Research Journal of Chemistry and Environment;Volume;30;Issue;2;pp.90-97
Date
01-01-2026
Publisher
World Researchers Associations
Subject
Adulteration; dexamethasone; herbal medicines; prednisolone; steroids; thin layer chromatography
Coverage
Rithu K., Department of Life Sciences, Christ University, Karnataka, Bangalore, 560029, India; Gayathri J., Department of Life Sciences, Christ University, Karnataka, Bangalore, 560029, India; Nag A., Department of Life Sciences, Christ University, Karnataka, Bangalore, 560029, India
Rights
Restricted Access; Hardcopy may be available in the library
Relation
ISSN: 9720626;
Format
online
Language
English
Type
Article

Citation

Rithu, K.; Gayathri, Jayasankar; Nag, Anish, “Development of a Thin Layer Chromatography method to detect the presence of spiked Steroid drugs in Herbal Crude extracts,” CHRIST (Deemed To Be University) Institutional Repository, accessed June 19, 2026, https://archives.christuniversity.in/items/show/23436.

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