ItemBook Chapter

Structure and mechanism of zinc-finger nucleases-mediated genome editing in plants

Title
Structure and mechanism of zinc-finger nucleases-mediated genome editing in plants
Creator
Chivukula, Jahnavi; Ponnachan, Blessy; Kumar, Dipanshu; Nagella, Praveen
Description
The chapter provides a comprehensive exploration of zinc-finger nucleases (ZFNs), intricate molecular tools designed to integrate genes of interest into target sites, thereby instigating both phenotypic and genotypic transformations. The focal point of this genetic manipulation lies in the activity of the FokI endonuclease enzyme, which, through the formation of homodimers, orchestrates precise cleavage at integration sites. Leveraging the wealth of genomic information across diverse organisms, the chapter elucidates the mechanistic underpinnings of ZFNs. A particular emphasis is placed on the modular assembly of ZFNs, unravelling the formation of ??? motifs and explicating the nuanced mechanisms governing their actions. The application of this methodology in plant engineering holds paramount significance, particularly in the realm of augmenting stress tolerance and nutritional value. The chapter systematically examines the gene of interest for various plants, including tobacco, soybean, Arabidopsis thaliana, rice, and wheat, elucidating the corresponding ZFN mechanisms. The amalgamation of sophisticated genetic tools with a detailed understanding of plant genomes presents a promising avenue for tailoring crops to meet diverse agricultural challenges. This work not only contributes to the fundamental understanding of ZFNs but also underscores their practical implications in advancing crop improvement strategies for sustainable agriculture. CAB International 2025. All rights reserved.
Source
Genome Editing for Crop Improvement: Theory and Methodology;pp.35-46
Date
01-01-2025
Publisher
CABI International
Subject
Crop plants; Endonuclease enzyme; Genome editing; Zinc-finger nucleases
Coverage
Chivukula J., Department of Life Sciences, School of Sciences, CHRIST (Deemed to be University), Bangalore, India; Ponnachan B., Department of Life Sciences, School of Sciences, CHRIST (Deemed to be University), Bangalore, India; Kumar D., Department of Life Sciences, School of Sciences, CHRIST (Deemed to be University), Bangalore, India; Nagella P., Department of Life Sciences, School of Sciences, CHRIST (Deemed to be University), Bangalore, India
Rights
Restricted Access; Hardcopy may be available in the library
Relation
ISBN: 978-180062250-0; 978-180062249-4;
Format
online
Language
English
Type
Book chapter

Citation

Chivukula, Jahnavi; Ponnachan, Blessy; Kumar, Dipanshu; Nagella, Praveen, “Structure and mechanism of zinc-finger nucleases-mediated genome editing in plants,” CHRIST (Deemed To Be University) Institutional Repository, accessed June 18, 2026, https://archives.christuniversity.in/items/show/24254.

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