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Isolation and characterization of plant growth promoting bacteria (PGPB) from the rhizosphere of Spinacea oleracea L.
As the years pass by, there is an increase in abiotic stress conditions around the environment that directly or indirectly affect agriculture around the world. Therefore, there is a dire need to increase the sustainability of plants. Plant Growth Promoting Bacteria (PGPB) play an important role in maintaining the physiology and growth of plants under various stress conditions. This study looks into the isolation and characterization of different PGPB from Spinacia oleracea L. and their tolerance against salinity and commonly used commercial pesticides against the Spinacia family. The techniques used are isolation by serial dilution, 16sRna sequencing, characterization of different PGPB assays for confirmation such as ammonia production, catalase test, phosphate solubilisation, potassium solubilization, siderophore production, indole-3-acetic acid production, biofilm formation assay, halotolerance and tolerance study using Minimal Inhibitory Concentration (MIC). PGPB were isolated and characterized from Spinacia oleracea L., which was under an abiotic stress environment. Isolates were Bacillus clarus, Bacillus licheniformis, Paenibacillus alvei SJ6 and Paenibacillus alvei SJ8, having quantities as high as 78.10.004 mgL-1 phosphate solubilization, 43.8 mgL?1 of indole-3-acetic acid production, 14.5660.011 psu of siderophore production and 0.62 0.027 mol mL?1 of ammonia production. All isolates also had considerable amounts of halotolerance up to 10%, whereas Bacillus licheniformis had 12.5% halotolerance. The bacterial isolates had considerable tolerance against commonly used commercial pesticides against green leafy vegetables such as chlorpyriphos + cypermethrin combination and fungicides such as mancozeb. Therefore, this study looks into the isolation of potential plant growth promoting bacteria that have considerable amount of halotolerance and pesticide tolerance. 2025 World Researchers Associations. All rights reserved. -
Isolation and characterization of plant growth promoting bacteria (PGPB) from the rhizosphere of Spinacea oleracea L.
As the years pass by, there is an increase in abiotic stress conditions around the environment that directly or indirectly affect agriculture around the world. Therefore, there is a dire need to increase the sustainability of plants. Plant Growth Promoting Bacteria (PGPB) play an important role in maintaining the physiology and growth of plants under various stress conditions. This study looks into the isolation and characterization of different PGPB from Spinacia oleracea L. and their tolerance against salinity and commonly used commercial pesticides against the Spinacia family. The techniques used are isolation by serial dilution, 16sRna sequencing, characterization of different PGPB assays for confirmation such as ammonia production, catalase test, phosphate solubilisation, potassium solubilization, siderophore production, indole-3-acetic acid production, biofilm formation assay, halotolerance and tolerance study using Minimal Inhibitory Concentration (MIC). PGPB were isolated and characterized from Spinacia oleracea L., which was under an abiotic stress environment. Isolates were Bacillus clarus, Bacillus licheniformis, Paenibacillus alvei SJ6 and Paenibacillus alvei SJ8, having quantities as high as 78.10.004 mgL-1 phosphate solubilization, 43.8 mgL?1 of indole-3-acetic acid production, 14.5660.011 psu of siderophore production and 0.62 0.027 mol mL?1 of ammonia production. All isolates also had considerable amounts of halotolerance up to 10%, whereas Bacillus licheniformis had 12.5% halotolerance. The bacterial isolates had considerable tolerance against commonly used commercial pesticides against green leafy vegetables such as chlorpyriphos + cypermethrin combination and fungicides such as mancozeb. Therefore, this study looks into the isolation of potential plant growth promoting bacteria that have considerable amount of halotolerance and pesticide tolerance. 2025 World Researchers Associations. All rights reserved. -
Isolation and characterization of polyhydroxyalkanoate producing halotolerant Bacillus subtilis SG1 using marine water samples collected from Calicut coast, Kerala
Halotolerant bacterial strain isolated from the Calicut coast, Kerala, India, was screened for its potential ability to synthesize polyhydroxyalkanoates (PHA) using Sudan black B and Nile blue staining. The quantitative analysis for PHA production was done in modified M9 media and a PHA yield of about 1.52 g/L was observed with the most potential isolate SG1. Further, the biochemical and molecular characterization of the PHA-producing halotolerant bacteria was done using standard biochemical tests and 16 s ribosomal RNA sequencing respectively and the isolate was identified to be Bacillus subtilis SG1. Further, the PHA recovery was done using solvent extraction method employing acetone and diethyl ether followed by precipitation using chloroform with a maximum PHA yield of 1.52 g/L. Further, the material properties of the extracted polymer were studied using Fourier transform infrared, X-ray diffraction, nuclear magnetic resonance, thermo gravimetric analysis, and differential scanning calorimetry analysis. Further investigations are necessary to optimize PHA production and to carry out its application study in various fields. 2024 Sneha Grigary, et al. -
Isolation and Screening of Chromium-Tolerant Bacteria from Wetland Rhizosphere for use in Sustainable Bioremediation
Heavy metal contamination by hexavalent chromium [Cr(VI)] remains a major ecological threat due to its toxicity and persistence. This study investigated chromium-tolerant rhizospheric bacteria associated with Typha domingensis collected from Doddagubbi Lake, Bengaluru. Twelve isolates were obtained and exhibited diverse colony morphologies, where Gram staining classified the isolates into six Gram-positive and six Gram-negative strains. Physiological screening revealed broad adaptability with several isolates (DL4, DL6, DL11) tolerating up to 7.5% NaCl and DL11 showing thermotolerance up to 50C. pH profiling indicated that multiple strains (DL2, DL4, DL6, DL7, DL8) sustained growth up to pH 10. Biochemical tests showed widespread catalase activity and notable proteolysis, citrate utilisation and amylase production. Molecular identification grouped the isolates into eight taxa, including Brevibacillus brevis, Bacillus subtilis, Paenibacillus cookii, Kocuria rhizophila and several Pseudomonas species. Chromium tolerance assays demonstrated clear differentiation between highly tolerant isolates (DL1, DL2, DL8, DL9), which showed uninterrupted growth from 10100 mg/L Cr(VI) and moderately tolerant isolates that declined beyond 60 mg/L. Quantitative OD??? measurements further confirmed that the highly tolerant group retained OD values >0.55 at 80100 mg/L, whereas sensitive strains dropped below 0.05. These results highlight the strong bioremediation potential of T. domingensisassociated rhizobacteria for Cr(VI)-contaminated environments. 2026, World Researchers Associations. All rights reserved. -
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Isolation of Fungal Endophytes From Hulimavu Lake Flora and Characterization and Optimization of Fungal Enzymes
The present work was aimed at isolating fungal endophytes from Hulimavu Lake, newlineextracting their secondary metabolites and fungal enzymes to subsequently probe the newlinebiological properties of these fungal endophytic bioactive compounds and enzymes. newlineHulimavu Lake, known for its notoriously high levels of pollution contributed by newlineanthropogenic factors, was chosen for this study with the literature-evidence backed newlinehypothesis that plant habitat stress also positively favors the type and quanta of bioactive compounds with novel features produced by its endophytes. Literature survey was performed to identify the probable types of plants found in this lake that could be used for the study and these were subsequently collected, identified and processed under laboratory conditions. These plants were authenticated by a certified botanist and were identified as Alternanthera philoxeroides, Ricinus communis and Persicaria glabra. Fungal endophytes were then isolated from different plant parts collected and were screened using preliminary LCB staining followed by DNA sequencing analysis. Based on ITS region sequencing, nucleotide homology and phylogenetic tree mapping, these fungal endophytic cultures were identified as Aspergillus niger, Talaromyces amestolkiae, Cladosporium phaseolorum and Diaporthe phaseolorum. Crude extracts obtained from these fungal endophytes displayed bacterial growth inhibition and significant free radical scavenging/ reduction potential that was comparable to standard ascorbic acid, hence depicting antioxidant activity of these extracts. Owing to their biological properties these crude extracts were further tested for cytotoxic properties on newlinedifferent models like that of Saccharomyces cerevisiae (Baker s yeast), Artemia salina newline(Brine shrimp) and MCF-7 cell line. The presence of fungal endophytic enzymes like newlineprotease, amylase, laccase and lipase was detected qualitatively and estimated newlinequantitatively. -
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Isolation of Plant Growth-Promoting Bacillus cereus from Soil and Its Use as a Microbial Inoculant
Modernization has introduced intensive agricultural practices wherein the pesticides play an important role both in stabilization and in increase of agricultural products. As a consequence, humans and members of other ecosystems are exposed to increased levels of compounds that have detrimental effects on their health, thereby signifying the importance of microbial inoculants. In order to achieve this goal 7 different bacterial species were initially screened for isolation of plant growth-promoting Bacillus sp. The isolate CUAMS116 was confirmed to be Bacillus cereus through biochemical and molecular characterization. The in vitro plant growth-promoting ability of the isolate was screened through standard tests. Different concentrations of bacterial inoculant (25%, 50%, 75%, 100%) were evaluated for its plant growth promotion ability using Phaseolus vulgaris L., under pot culture conditions. At the harvest stage, the mature control plants measured 16.53cm and mean treated plant height was measured to be 27.75cm, showing a maximum percentage increase in length of 67.87%. The results suggested that the B. cereus CUAMS116 isolated in this study can be extended as a PGPM through further field trials in other plants for improving crop yield and tolerance to biotic and abiotic stresses. 2020, King Fahd University of Petroleum & Minerals. -
Isolation, Characterization and Evaluation of Potential Plant Growth-Promoting Bacteria From The Rhizosphere of Coffea arabica L. From Kodagu District, Karnataka
Agriculture is fundamental to food security and economic stability. The utilisation of newlineplant growth-promoting rhizobacteria (PGPR) an eco-friendly alternative to chemical newlinefertilisers is particularly promising as these microorganisms enhance nutrient newlineavailability, stimulate plant growth, and improve resistance to biotic and abiotic newlinestresses. This study aimed to isolate and characterise PGPR from the rhizosphere of newlineCoffea arabica L. in the Kodagu District of Karnataka, focusing on their potential as newlinebiofertilizers. Soil samples collected from ten different locations within Kamath Coffee newlineEstate led to the isolation of fourteen distinct bacterial colonies, labelled NJ01 to NJ14. These isolates were evaluated for various plant growth-promoting (PGP) traits like indole-3-acetic acid (IAA) siderophore and ammonia production, nitrogen fixation, newlinephosphate, and zinc solubilization and hydrogen cyanide (HCN) production. Further newlinestudies were carried out using 3 isolates with significant PGP traits. Enzyme activity newlineassays indicated that NJ01, NJ04, and NJ14 had positive results for protease, cellulase, newlineamylase, and catalase activity. These strains also displayed varying degrees of newlinehalotolerance, with NJ01, NJ04 and NJ14 tolerating up to 8%, 10% and 11% NaCl, newlinerespectively. Heavy metal tolerance tests revealed that NJ01 tolerated Pb up to 250 newlineg/mL, NJ04 tolerated Pb up to 1500 g/mL and Cd up to 50 g/mL, and NJ14 tolerated Pb up to 1000 g/mL and Hg up to 100 g/mL. Polyhydroxyalkanoate (PHA) production was confirmed in all isolates, with NJ01 yielding the highest amount (6.4 g/L). The isolates exhibited significant antifungal and the 16S rRNA sequencing identified NJ01 as Bacillus albus (OP784795), NJ04 as Bacillus licheniformis (OM780221), and NJ14 as Bacillus subtilis (OM780222). A consortium was prepared after confirming the plant growth promoting abilities of these PGPR strains by carrying out an initial plant growth studies. The optimisation of media parameters was by using a two-factor interaction model. -
Isolation, Identification and Characterization of A Potential Probiotic Bacteria and its Application in Aquaculture
In the current study, Bacillus subtilis PKB1 was isolated from dried anchovies and identified using morphological and biochemical analysis followed by 16S rRNA sequencing and phylogenetic analysis. The antibacterial and anti- haemolytic properties of the bacteria were also examined. Probiotic qualities were assessed by measuring the isolated strain's tolerance to artificial gastric juice. To assess the isolated strain's capacity for stress tolerance, additional exposure to varied pH, temperature, and organic solvent concentrations was given. Bacillus subtilis in MRS medium produced exopolysaccharide (EPS) which was purified and a maximum yield of 1928mg/L was achieved. The novel exopolysaccharide was purified and characterized to be a heteropolysaccharide. The polysaccharide present had a molecular weight of about 20 kDa overall. FTIR spectroscopy revealed the presence of carboxyl, hydroxyl, and methylene groups in the EPS. The extremely cross-linked structure of the EPS with lumps that resembled spikes was revealed by atomic force microscopy. Scanning Electron Microscopy (SEM) revealed that it possessed rough, asymmetrical lumps. Emulsification activity was also observed in the EPS. The current study's findings suggested that this EPS might make a good candidate for further applications. Utilizing probiotics derived from unconventional sources may improve the variety of probiotics accessible for use in aquaculture procedures. Probiotics are used in the field of aquaculture in order to improve immunity and to protect against pathogenic microbes. Species such as Aeromonas hydrophila and Vibrio parahaemolyticus are responsible for increasing mortality, decreasing production efficiency and forcing antibiotic overuse. In this study, the role of the isolated probiotic in promoting growth performance in Nile tilapia was examined using growth and challenge studies. The experiment was conducted for a duration of 30 days. Weight gain, histology and mortality rates were studied in the presence and absence of pathogenic microbes. Bacillus subtillis PKB1 supplementation for Nile tilapia helped to maintain the integrity of gill filaments and improved survivability and weight gain when used at a concentration of 1x104 CFU/ml. The study showed that the isolate improved the survival rate of Nile Tilapia against Vibrio parahaemolyticus and Aeromonas hydrophila. -
Isolation, Screening and Characterisation of Endophytes from Madiwala Lake for Biosurfactant, Bioremediation and Plant-Growth Promotion Properties
The significant surge in population, combined with the degradation of the environment, has imposed substantial stress on worldwide food newlinesecurity. The concerning pace of population growth, along with escalating environmental harm due to heightened industrialization, newlinehas indeed exerted considerable pressure on the global food provision. Considering the existing situation, the sustainable approach to enhance agricultural yield and facilitate environmental bioremediation entails utilizing endophytes that reside within plants. Endophytic microorganisms possessing the capacity to promote plant growth and exert biocontrol can significantly boost plant development amidst fluctuating environmental factors, both biotic and abiotic in nature. The current research aimed to extract bacterial and fungal endophytes from Alternanthera philoxeroides and newlineAlternanthera paranichoides and evaluate their potential for enhancing plant growth and controlling pests. Among the isolated newlineendophytic bacteria, Klebsiella pneumoniae exhibited various characteristics conducive to plant growth, leading to enhanced newlinegermination and vegetative growth in Vigna unguiculata plants. The isolate exhibited good Indoleacetic acid (IAA) production newline(48.752.95 g/mL) and potassium solubilization (2.130.07 ppm). The IAA production by K. pneumoniae was further enhanced by 4- fold using the RSM optimization to 195.662.51 g/mL. The newlineendophytic bacteria Bacillus amyloliquefaciens and Bacillus subtilis newlineshowed good extracellular enzyme production and antimicrobial activity along with plant growth promotion. The endophytic bacterium B. amyloliquefaciens showed good newlinebiosurfactant production and bioremediation efficiency. The strain displayed notable resistance to Cr and Pb concentrations upto 2000 mg/L. It was found to possess maximum metal removal efficiency for Pb, 92.3% at pH 9 and 86.2 at 25 oC. -
ISOMORPHIC DECOMPOSITION OF GRAPHS OF SEMI-PRIME SIZE
In this dissertation, we study the condition required for a graph of semi-prime size to have a Sn decomposition. We have considered the simplest cases with the semi-prime value as 15, which is the product of 3 and 5; graph being a tree; and S4 decomposition is considered. Any graph can be decomposed into subgraphs, but the subgraphs obtained after decomposition need not be isomorphic to each other. We discuss, under what conditions can a tree of size 15 be decomposed such that all the subgraphs obtained is isomorphic to S4. -
Isothermal autocatalysis of homogeneousheterogeneous chemical reaction in the nanofluid flowing in a diverging channel in the presence of bioconvection
The nonlinear differential equations play a prominent role in the mathematical description of many phenomena that occur in our world. A similar set of equations appear in this paper that govern the homogeneous and heterogeneous chemical reactions in the nanofluid flowing between two non-parallel walls. Since the concentration of the homogeneous species is substantially high, quartic autocatalysis is considered for the analysis. It is found to be more effective than the cubic autocatalysis. Further, to avoid the deposition of nanoparticles on the surface, self-propelled microorganisms called gyrotactic microorganisms are allowed to swim in the nanofluid. This movement of microorganisms constitutes a major phenomenon called bioconvection. The set of governing equations thus formed are made dimensionless and the resulting system of equations are solved by Differential Transformation Method (DTM) with the help of Padapproximant that reduces the power series into rational function. This transformation helps in achieving a better convergence rate. The fluid flow analysis is interpreted through graphs and tables where it is observed that the heat source enhances the temperature of the nanofluid. Further, the homogeneous and heterogeneous chemical reaction parameters have significant impacts on the concentration of the reactants. Also, the outcomes indicated that the reaction profiles and motile density profiles increase with the increase in Schmidt number. 2021 Informa UK Limited, trading as Taylor & Francis Group. -
It is their duty: a commentary on filial discrepancy and perceived neglect among older adults
Filial piety, a traditional value that dominates the family structure in many societies, plays a significant role in the well-being of the geriatric population. The gravity of a healthy filial relation is weighed out in the existing literature, by highlighting correlations with the onset of depression, loneliness, low life-satisfaction and suicidal tendencies. Grounded in the expectations of respect, care and obedience, these values have been challenged by the changing family dynamics of the modern world. This commentary explores how such discrepancies could instill a perception of neglect and abuse among the older population, thereby affecting their quality of life. 2025 Taylor & Francis Group, LLC. -
IT Strategies for Effective Marketing in Globally Diverse Corporate Environments
Today, organizations discern multicultural teams, dynamic consumer tastes and shrinking landscapes of competition bordering on the internet-centric global economy. This paper investigates the role of IT strategies in improving the strategic marketing within different corporate environments. An examination of IT's role in addressing marketing and management complexity in different cultural context is made. Theoretical models are reviewed and responsible global marketing practices are promoted through digital transformation to reshapes the business operations. We also illustrate IT based solutions for dealing with cross cultural communication barriers, resistance to change as well as team dynamics. In this case study and trend analysis with trends, we show how own best practices of market segments, digitalization as well as cross cultural management work together with IT to encourage agility, customer focus, and continuous learning in the organization. 2025 IEEE. -
It-Driven Governance Models for Social Impact and Community Development
Local development planning in India is impaired by fragmented administrative datasets, limited citizen participation, and opaque decision processes despite the availability of India Stack primitives and Open Government Data (OGD) such as NFHS and MGNREGA. This study aimed to design, implement, and evaluate Federated Neuro-Symbolic Governance (FNSG), a privacy-preserving cross-silo framework that couples neural concept learners with symbolic rule synthesis, to deliver interpretable district-level decision support. FNSG operates in a hierarchical federated topology (district clients, state aggregator) using NFHS-5, MGNREGA, census/SECC proxies, and geospatial layers. Local clients train neural concept encoders, extract symbolic rule candidates, and transmit clipped, DP-noised updates plus anonymized rule evidence for secure aggregation and global rule distillation. In pilot experiments (K=20 districts, geographic holdout) FNSG achieved mean test AUC 0.84 versus centralized 0.88 and FedAvg 0.80, Precision@100 0.69, and produced concise global rules with fidelity ? 0.72. Privacy - utility sweeps indicate acceptable utility at ? ? 2 (AUC ? 0.82), with degradation at ? ? 1; convergence required ? 110 rounds and per-epoch client time ? 18 s on CPU (? 8 s on GPU). FNSG balances utility, interpretability, and data sovereignty. Policy implications include staged pilots, capacity building for district data centers, mandatory audit logs, and India Stack-mediated consent and integration. 2026 IEEE. -
It's Liquidation Time at Bhoruka Gases: A Case Study
GFJMR Journal of Management and Research, Vol-6, pp. 78-92. ISSN-2229-4651 -
ITBC Controlled IPWM for Solar Based Wide Range Voltage Conversion System
This paper presents an isolated tiny or micro-bidirectional DCAC converter (ITBC) that operates in a single-phase, single-stage, bidirectional, and isolated configuration. The converter utilises a voltage-in-phase PWM (IPWM) control scheme to regulate the potential conversion ratio of the converter, enabling it to handle a wide voltage range. The converter also enables smooth power flow direction changes and can achieve soft switching, resulting in high efficiency. A unidirectional current controller can be used for both DCAC and ACDC modes. The paper provides detailed information about the micro-inverter's working principles, design procedure, characteristics, and control strategy. The proposed solution is verified by a 500-watt test bench model with a voltage input range of 30 volts to 50 volts. 2024 IETE. -
ITERATED LINE GRAPHS WITH ONLY NEGATIVE EIGENVALUES ?2, THEIR COMPLEMENTS AND ENERGY
In this article, several iterated line graphs Lk (G) with all equal negative eigenvalues ?2 are characterized for k ? 1 and their energy consequences are presented. Also the spectra and the energy of complement of these graphs are obtained, interestingly they have exactly two positive eigenvalues with different multiplicities. Moreover, we characterize a large class of equienergetic graphs which extend some of the existing results. Palestine Polytechnic University-PPU 2025. -
J. R. D. Tata
J. R. D. Tata, commemorative postage stamp was originally issued by the Department of Posts on 29 November, 1994 on his first death anniversary. He is the first licensed pilot in India and awardee of numerous national and international honors.