In Vitro Production of Tocopherols

Title

In Vitro Production of Tocopherols

Creator

Lakshmaiah V.V.; Joseph B.V.; Bhaskar R.; Ulhas R.S.; Al-Khayri J.M.; Nagella P.

Description

Tocopherols are an essential dietary nutrient for mammals and photosynthetic products produced by green plants. Tocopherols commonly referred to as vitamin E exist in four forms (?-, ?-, ?-, and ?-tocopherol). Synthetic ?-tocopherol is a mixture of eight racemic forms and is less effective than natural tocopherol, thus the demand for plant-derived tocopherols is high. Tocopherols are lipophilic antioxidant and extensively used as therapeutic agents such as anti-inflammatory, anti-infection, anticancer, immune-stimulant, and nephro-protectant. They are also used as food additives and nutraceuticals. Plant cell and tissue culture is one of the promising techniques for mass production of tocopherols to meet the commercial demand. Optimizing physical and chemical factors for in vitro culture system has resulted in better accumulation of the product. Moreover, using bioreactors, precursor feeding, elicitation, biotransformation, and metabolic engineering approaches have resulted in enhanced yield of tocopherols from in vitro cultures. The present chapter deals with various important aspects of tocopherol in vitro production such as biosynthesis of tocopherol with special emphasis on key enzymes involved in the pathway whose modulation in expression can increase the yield of the product. Topics discussed include production of tocopherol from callus, cell and organ culture, metabolic engineering for mass production, different methods employed for extraction and quantification of tocopherols, and their biological activities and commercial applications. The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Singapore Pte Ltd. 2022.

Source

Nutraceuticals Production from Plant Cell Factory, pp. 287-319.

Date

2022-01-01

Publisher

Springer Nature

Subject

Biological activity; Biosynthesis; Elicitation strategies; Metabolic engineering; Plant cell and organ culture; Tocopherol

Coverage

Lakshmaiah V.V., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bengaluru, India; Joseph B.V., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bengaluru, India; Bhaskar R., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bengaluru, India; Ulhas R.S., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bengaluru, India; Al-Khayri J.M., Department of Agricultural Biotechnology, College of Agriculture and Food Sciences, King Faisal University, Al-Ahsa, Saudi Arabia; Nagella P., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bengaluru, India

Rights

Restricted Access

Relation

ISBN: 978-981168858-4; 978-981168857-7

Format

Online

Language

English

Type

Book chapter

Identifier

https://doi.org/10.1007/978-981-16-8858-4_12

https://www.scopus.com/inward/record.uri?eid=2-s2.0-85160113306&doi=10.1007%2f978-981-16-8858-4_12&partnerID=40&md5=8045cc47499d53a31bce269ed402091e