Role of plant tissue culture medium components

Title

Role of plant tissue culture medium components

Creator

Sudheer W.N.; Praveen N.; Al-Khayri J.M.; Jain S.M.

Description

The plant tissue culture technique is one of the most acquainted techniques and has been practiced since 1902 for the development of disease-free plants with desired quality, at a larger production rate within a short span of time, irrespective of the season. Each plant has its own morphology and nutritional requirements. Moreover, the nutritional requirement for the tissues in each part of the plant varies. Thus, for the optimal growth of the desired plant, the composition and choice of the suitable media are crucial factors. The formulation of the nutrient medium for the specific tissue and the specific plant plays a vital role in the development of suitable plant tissue culture techniques. This chapter discusses the main components of the plant tissue culture media: basic components which include macroelements, microelements, nitrogen sources (including amino acids), carbon sources, organic supplements (mainly vitamins and other supplements), growth regulators like auxins, cytokinins, gibberellins, abscisic acid, ethylene, and solidifying agents. The basic components have to be incorporated at a fixed concentration. The plant growth regulators are supplemented for organogenesis and the balance between the growth regulators depends on the type of tissue culture technique adopted. The additional supplements are incorporated into the media to achieve specific responses which include activated charcoal, antioxidants, ethylene inhibitors, and complex organic additives, and antibiotics/antifungal agents are supplemented to prevent plant loss due to contamination. The chapter also emphasizes the sterilization techniques and their importance in the plant tissue culture medium. The significance of the adoption of automated media preparation and incorporation of nanoparticles in the tissue culture media is also discussed. 2022 Elsevier Inc. All rights reserved.

Source

Advances in Plant Tissue Culture: Current Developments and Future Trends, pp. 51-83.

Date

2022-01-01

Publisher

Elsevier

Subject

Carbon sources; Nanoparticles; Organic additives; Plant growth regulators; Plant tissue culture medium; Sterilization

Coverage

Sudheer W.N., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bangalore, India; Praveen N., Department of Life Sciences, CHRIST (Deemed to be University), Karnataka, Bangalore, India; Al-Khayri J.M., Department of Agricultural Biotechnology, College of Agriculture and Food Sciences, King Faisal University, Al-Ahsa, Saudi Arabia; Jain S.M., Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland

Rights

Restricted Access

Relation

ISBN: 978-032390795-8; 978-032399819-2

Format

Online

Language

English

Type

Book chapter

Identifier

https://doi.org/10.1016/B978-0-323-90795-8.00012-6

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